Microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof

A technology of microbial preparations and soil-borne fungi, which is applied in the direction of plant growth regulators, plant growth regulators, biocides, etc., can solve the problems of affecting the ecological environment, slowness, and easy resistance to chemical pesticides, so as to promote the growth of seedlings Effect

Inactive Publication Date: 2009-09-02
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While soil fumigation kills pathogenic bacteria, it also kills beneficial microorganisms in the soil and destroys the micro-ecological balance; while the development of resistant varieties is slow due to problems such as sing...

Method used

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  • Microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Example 1 liquid fermentation preparation production

[0018] 1. Activation of strains

[0019] The bacterial strain Da07210 was inoculated onto the solid medium, and the medium formula was 20.0 g of soluble starch, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, 20.0g agar, (make up 1000ml), pH 7.2, culture at 25℃~28℃ for 4-5 days.

[0020] 2. Primary seed cultivation

[0021] Inoculate the activated strain into the liquid medium, the formula is glucose 10.0g, soybean powder 10.0g, yeast extract 10.0g, soluble starch 5.0g, KH 2 PO 4 0.5 g, distilled water (to make up 1000 ml), pH 7.2, shake the flask at 25°C to 28°C for 48 hours to obtain the primary seed liquid.

[0022] 3. Secondary seed cultivation

[0023] The primary seed liquid is inoculated into the culture medium, and the medium formula is the same as that of the aforementioned primary seed culture (2). Cultivate at 26°C-28°C for 48 hours to obtain secondary seed liquid for...

example 2

[0026] The production of example 2 solid biological preparations

[0027] First, strain Da07210 was produced by liquid fermentation (same as Example 1). Then add filler, plate and frame filtration, beating, drying, crushing to make soluble powder, the specific process is as follows:

[0028] Specifically add the filler: put the fermentation hydraulic pressure into the storage tank, add diatomaceous earth (light calcium carbonate) as the filler according to the following calculation formula, and stir for 30 minutes after adding.

[0029] The amount of filler diatomite (light calcium carbonate) added (kg) = [number of bacteria in the fermentation broth (100 million / ml) × volume of the tank (liter) × yield] / number of bacteria in the finished product - the residue in the fermentation broth (Kilogram).

[0030] Plate and frame filtration: With a pressure of 2 kg / cm2, the material is pressed from the storage tank into the plate and frame, and filtered through No. 7 filter cloth....

example 3

[0035] Example 3 solid preparation is to field control effect of banana fusarium wilt

[0036] 1. Medicament for test: biocontrol bacteria Da07210, wettable powder (solid preparation), and 30% hymexazol water as contrast fungicide.

[0037] 2. Preparation of the pathogenic bacteria of Fusarium wilt of banana: inoculate FOC4 in PDA solid medium, after culturing for 7 days, prepare spore suspension with sterilized water at a concentration of 10 6pieces / ml.

[0038] 3. Test location: Jinjiang Banana Base in Hainan Province.

[0039] 4. Application method: Dilute the solid preparation 300 times with clean water, carry out root irrigation treatment, and then use banana wilt pathogen spore suspension to irrigate the roots after 3 days, and set clean water treatment and pathogenic bacteria treatment. The investigation was carried out 3 months after the root irrigation of pathogenic bacteria, the disease index and incidence rate of each treatment were recorded, and the control effec...

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Abstract

The invention relates to a microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof, which belong to the field of microbial pesticide. The microbial preparation is prepared by a conventional method. A production strain of the microbial preparation is actinomyces Da07210 which is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the actinomyces Da07210 is CGMCC No.2561. The strain is prepared into a liquid preparation through liquid fermentation, or is prepared into wettable powder by mixing the strain with an adsorption carrier according to a certain proportion, filtering, concentrating, grinding and airing the mixture. The microbial preparation has the advantages that the microbial preparation can prevent and control banana wilt, plague, seedling blight, damping-off and the like caused by fusarium, phytophthora, rhizoctonia and pythium pathogenic fungi, and can also stimulate root systems of plants to grow and promote the growth of plant seedlings.

Description

technical field [0001] The present invention relates to the application of an actinomycete in the prevention and control of plant soil-borne fungal diseases, in particular to the application of an actinomycete which has a strong antagonistic effect on pathogenic fungi such as Fusarium and Phytophthora in the prevention and control of plant soil-borne fungal diseases At the same time, it can stimulate the growth of plant roots and promote the growth of plant seedlings. The invention belongs to the field of biological control of crop diseases by using microorganisms. Background technique [0002] Plant soil-borne fungal diseases are a kind of important plant diseases that are difficult to control. There are many kinds of pathogenic fungi, which belong to Phytophthora, Fusarium, Sclerotinia, Rhizoctonia, Pythium, etc. They live in In the soil, when the conditions are suitable, the plants are infected from the roots or stems of the plants, and the soil is used as a medium to sp...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01P21/00
Inventor 鲍时翔朱军黄惠琴吴晓鹏方哲郑业辉孙前光
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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