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Polysaccharide-liposome and preparation method and purpose thereof

A liposome and polysaccharide technology, which is applied in the preparation of polysaccharide liposomes and the field of lentinan liposomes, can solve difficult problems and achieve obvious targeting effects

Active Publication Date: 2011-04-13
WUHAN DOCAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is more difficult to obtain polysaccharide liposomes with higher drug encapsulation efficiency and smaller particle size

Method used

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  • Polysaccharide-liposome and preparation method and purpose thereof
  • Polysaccharide-liposome and preparation method and purpose thereof
  • Polysaccharide-liposome and preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Preparation of Liposome 1:

[0063] Weigh 8.0 g of soybean lecithin, 1.0 g of cholesterol, 0.2 g of polyethylene glycol 2000 and 0.2 g of vitamin E, add 900 ml of chloroform, stir and dissolve to obtain an oil phase mixture. Weigh 0.1g injection-grade lentinan and 0.9g sodium deoxycholate, add 300ml of water for injection, and add an appropriate amount of 0.1mol / L sodium hydroxide solution until the lentinan dissolves, and adjust the pH of the solution to 7.0 with 0.1mol / L hydrochloric acid ~8.0, an aqueous phase mixture was obtained. Under cooling in an ice-water bath, slowly add the water phase mixture into the oil phase mixture, and then ultrasonically emulsify intermittently for about 5 minutes to obtain a milky white uniform W / O emulsion. The solvent was removed by rotary evaporation under reduced pressure at 40°C under the protection of nitrogen to obtain a pale yellow thick jelly, which was added with 200ml of 0.9% sodium chloride solution and incubated a...

Embodiment 2

[0074] Preparation: Weigh 8.0 g of soybean lecithin, 1.0 g of cholesterol, 0.2 g of polyethylene glycol 2000 and 0.2 g of vitamin E, add 900 ml of chloroform, stir to dissolve, and obtain an oil phase mixture. Weigh 0.1g of injection-grade lentinan and 0.9g of sodium deoxycholate, add 300ml of water for injection, and add an appropriate amount of 0.1mol / L sodium hydroxide solution until the lentinan dissolves, and adjust the pH value to 7.0~ with 0.1mol / L hydrochloric acid 8.0, an aqueous phase mixture was obtained. Under cooling in an ice-water bath, slowly add the water phase mixture into the oil phase mixture, and then ultrasonically emulsify intermittently for about 5 minutes to obtain a milky white uniform W / O emulsion. Remove the solvent by rotary evaporation under reduced pressure at 40°C under the protection of nitrogen to obtain a light yellow thick jelly, add 200ml of 2% mannitol in phosphate buffer (pH 6.0-6.5), and incubate at 40°C for 1h to obtain liposome ...

Embodiment 3

[0080] Preparation: Weigh 6.1g of soybean lecithin, 0.8g of cholesterol, 0.2g of polyethylene glycol 1000 and 0.2g of vitamin E, add 900ml of chloroform, stir to dissolve, and obtain an oil phase mixture. Weigh 0.1g of injection-grade lentinan and 0.5g of sodium deoxycholate, add 300ml of water for injection, and add an appropriate amount of 0.1mol / L sodium hydroxide solution until the lentinan dissolves, and adjust the pH value to 7.0~ with 0.1mol / L hydrochloric acid 8.0, an aqueous phase mixture was obtained. Under cooling in an ice-water bath, slowly add the water phase mixture into the oil phase mixture, and then ultrasonically emulsify intermittently for about 6 minutes to obtain a milky white uniform W / O emulsion. The solvent was removed by rotary evaporation under reduced pressure at 35°C under the protection of nitrogen to obtain a light yellow thick jelly. Add 200ml of phosphate buffer solution with a pH value of 6.0 to 6.5, and incubate at 40°C for 1.5h to obtain a l...

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Abstract

The invention relates to a polysaccharide-liposome which is prepared from raw materials such as polysaccharide, phosphatides, cholesterol, surface active agent and polyethyleneglycol. The liposome has smaller grain diameter and higher envelop rate. The invention particularly relates to a lentinan liposome and provides a preparation method of the polysaccharide-liposome and a purpose of the polysaccharide-liposome used for preparing drugs with the functions of antitumor or immunity adjustment.

Description

technical field [0001] The invention relates to a polysaccharide liposome, in particular to a lentinan liposome; the invention also relates to a preparation method and application of the polysaccharide liposome. Background technique [0002] Polysaccharides are ubiquitous in nature, they are an important part of living organisms, and they are related to a variety of physiological functions required to maintain life. Polysaccharides have unique effects in anti-tumor, anti-infection, cardiovascular disease, glucose metabolism, anti-aging, etc., and can improve the body's immune function. [0003] However, in the actual clinical application process, in many cases, polysaccharide drugs cannot achieve the expected ideal pharmacological activity in vivo. This is mainly due to the fact that most of the polysaccharide drugs have been metabolized before reaching the lesion site in the body, and this type of drug is not targeted to the lesion site. Therefore, how to make the polysac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K31/715A61P35/00A61P31/00A61P9/00A61P3/10
Inventor 陈邦银张文军
Owner WUHAN DOCAN PHARMA
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