Novel method for extracting anthocyanin in bilberry by double water phases

An anthocyanin and two-phase technology, applied in the field of bioengineering, can solve the problems of low extraction efficiency, large amount of solvent, and high cost, and achieve the effects of improving utilization rate, reducing production cost, and fast separation

Inactive Publication Date: 2009-09-16
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved in the present invention is to extract anthocyanins from the bilberry in the prior art. ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: We adopt the cranberry fruit that is more common and easy to take as raw material

[0016] Step 1: crush and homogenize cranberry fruit, and obtain fruit residue after suction filtration.

[0017] Step 2 Take 1.6g of anhydrous ammonium sulfate and 5.0g of water with a pH value of 3.03 to prepare an ammonium sulfate solution.

[0018] Step 3 Add the above ammonium sulfate solution and 3.4 g of absolute ethanol to 0.5 g of cranberry fruit residue, stir evenly to form a new two-phase aqueous system, and stand at room temperature for phase separation for 1 h. The distribution coefficient of anthocyanin is 6.63, and the distribution rate in the upper phase is 90%. The yield is 0.91 times that of the acidified ethanol method.

Embodiment 2

[0020] Step 1: Take bilberry fruit, crush and homogenate it, and filter it with suction to obtain the fruit residue.

[0021] Step 2 Take 1.9g of anhydrous ammonium sulfate and 5.1g of water with a pH value of 3.53 to prepare an ammonium sulfate solution.

[0022] Step 3: Add the above-mentioned ammonium sulfate solution and 3.0 g of absolute ethanol to 1.0 g of cranberry fruit residue, stir evenly to form a new two-phase aqueous system, and stand at room temperature for phase separation for 4 hours. The distribution coefficient of anthocyanin is 7.06, and the distribution rate in the upper phase is 93%, and the yield is 0.92 times that of the acidified ethanol method.

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PUM

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Abstract

The invention relates to a novel method for extracting anthocyanin in bilberry by double water phases, and belongs to the technical field of biological engineering. The method is characterized by comprising the following steps: adding inorganic salt solution and hydrophilic low-molecular organic substance into bilberry fruit residue, stirring the mixture evenly, keeping the mixture standing, layering the mixture, extracting the anthocyanin to an upper phase, and distributing the residue at the lower phase or between the two phases so as to achieve the aim of extracting the anthocyanin from the bilberry fruit residue. The inorganic salt is one or a combination of sodium chloride, ammonium sulphate, sodium sulfate, sodium carbonate, potassium carbonate, potassium phosphate, monopotassium phosphate and dipotassium hydrogen phosphate, preferably ammonium sulphate; and the hydrophilic low-molecular organic substance is one or a combination of methanol, ethanol, normal propyl alcohol, isopropyl alcohol, normal butanol, isobutyl alcohol, glycol and acetone, preferably ethanol. The method has the advantages that a novel method for extracting the anthocyanin is developed, the method is easy to industrialize, the utilization ratio of the bilberry is improved, and the production cost of the anthocyanin is lowered.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a method for extracting anthocyanins from bilberry, in particular to a novel two-phase aqueous phase extraction method for anthocyanins in bilberry fruit residues. Background technique [0002] Anthocyanins are a class of water-soluble pigments widely present in plants and belong to flavonoid compounds. It is mostly used in beverages, ice cream, jams, candies and pastries. It is a functional food additive and can also be used as medicines and cosmetics. As a natural pigment, anthocyanins have high safety performance, and have functions such as anti-mutation, anti-oxidation, lowering blood pressure, and protecting the liver. Pigment extracts with an anthocyanin content greater than 24% are used as medicines in Europe, and the extract of European bilberry anthocyanins (Mytrocyan) has been recorded in the pharmacopoeias of Italy, Germany and other countries. [0003] At presen...

Claims

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Application Information

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IPC IPC(8): C07D311/62A23L1/30A23L1/275A61P35/00A61P39/06A61P9/12A61P1/16
Inventor 修志龙张华许鸽李梦露都丽娅
Owner DALIAN UNIV OF TECH
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