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Preparation method of biological feed additive

Inactive Publication Date: 2009-09-30
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nevertheless, with the development of modern livestock and poultry breeding industry and the improvement of people's living standards, the requirements for the speed and quality of livestock and poultry breeding are also getting higher and higher. The existing feed additives can no longer meet the requirements of livestock and poultry breeding.

Method used

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  • Preparation method of biological feed additive
  • Preparation method of biological feed additive
  • Preparation method of biological feed additive

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0025] 1. Select pine needles, astragalus, and hawthorn three kinds of traditional Chinese medicines and mix them with ginkgo leaves for fermentation, and select appropriate Chinese medicines and their ratios with ginkgo leaves and bran.

[0026] 2. The experiment adopts a two-factor completely randomized design, a control is set, a total of 10 treatments are repeated 3 times, and the experimental design is shown in Table 1.

[0027] Table 1 Two-factor complete random design table

[0028]

[0029] Table 2 Fermentation comparison of different traditional Chinese medicine-Ginkgo biloba combinations

[0030]

[0031] Note: Different lowercase letters indicate significant differences (P<0.05), and different uppercase letters indicate extremely significant differences (P<0.01).

[0032] 3. The test results show that the effect of fermenting with Astragalus-Ginkgo biloba-bran as the fermentation raw material is better (Table 2). The fermented product of pine needles-gingko ...

preparation Embodiment 2

[0037] 1. Transfer the strain of Candida rutans to the slant medium and culture it at 28°C for 20 hours. The above-mentioned slant medium is PDA medium. The preparation process is as follows: After 200g of potatoes are boiled in water, add 20g of sucrose and 10g of agar , mix well, add water to make up to 1000mL.

[0038] 2. Inoculate the Candida utilis obtained in step (1) into the liquid seed medium on a clean bench, and culture on a shaker (28° C., 200 rpm) for 24 hours. The preparation process of the liquid seed medium is as follows: 20 g of glucose, 10 g of peptone, and 10 g of yeast extract are dissolved in water, and then the volume is adjusted to 1000 mL with water. Liquid culture is based on sterilization at 121°C for 20 minutes. The content of saccharomycetes in the gained seed culture liquid is 9 * 10 9 pc / mL, stored in a refrigerator at 4°C for 1 month.

[0039] 3. Dry and grind astragalus, ginkgo leaves, and bran into fine powder, sift through a 40-mesh sieve, ...

preparation Embodiment 3

[0045] 1. Transfer the strain of Candida rutans to the slant medium and culture it at 25°C for 24 hours. The above slant medium is PDA medium. The preparation process is as follows: Cut 200g of potatoes into small pieces and boil them in water And maintain for 20-30 minutes, filter with double-layer gauze to get potato juice, add 15g of sucrose and stir to dissolve, then add 20g of agar, continue to heat until the agar is completely melted and becomes transparent, and finally add water to make up to 1000mL.

[0046] 2. Inoculate the Candida utilis obtained in step (1) into the liquid seed medium on a clean bench, and culture it on a shaking table (25° C., 200 rpm) for 30 h. The formula of the liquid seed medium is: glucose 20g, peptone 6g, yeast extract 10g, dissolve in distilled water and dilute to 1000mL. Liquid culture is based on sterilization at 121°C for 20 minutes. The content of saccharomycetes in the gained seed culture liquid is 6 * 10 9 pc / mL, stored in a refriger...

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Abstract

The invention relates to a preparation method of biological feed additive which is used for livestock and poultry breeding and can effectively enhance the breeding speed and quality. The preparation method of the biological feed additive is as follows: pulverizing milk veteh, ginkgo leaf and bran into powder, evenly mixing the powder with nutrient fluid to prepare solid culture medium, and fermenting the mixture by Candida utilis to obtain the biological feed additive, wherein the mass ratio of the milk veteh, the ginkgo leaf and the bran is 1:2-4:1; and the preparation method of the nutrient fluid is as follows: dissolving 2.47-3g of cane sugar, 3.29-4g of NH42SO4, 2g of KH2PO4 and 0.5g of MgSO4.7H2O into water and metering the volume to be 100mL with water. The biological feed additive prepared has high nutrition, is rich in amylase, vitamins, minerals, digestive enzyme, somatomedin and relatively complete amino acid, has good palatability and can effectively enhance the breeding speed and quality.

Description

technical field [0001] The invention relates to a preparation method of a biological feed additive, which belongs to the field of animal feed additives. Background technique [0002] Since ancient times in my country, there has been a record of mixing traditional Chinese medicine into feed to treat diseases, and there are tens of thousands of clinically proven effective prescriptions. Chinese herbal medicine is a natural medicine, which can promote the growth and development of livestock and poultry, improve the immune function of the body, and prevent and treat diseases. It also has the advantages of wide source of medicine, low price, good effect, no side effects, and no residue. Astragalus membranaceus is mild in nature, sweet in taste, and has the effects of invigorating qi and solidifying the surface, antiperspirant and detoxification, promoting muscle growth, diuresis, and reducing swelling. The chemical components of Astragalus mainly include flavonoids, saponins and...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K10/12A23K20/147A23K20/163A23K40/00
Inventor 曹福亮金丽杨小虎
Owner NANJING FORESTRY UNIV
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