Preparation method of biological feed additive

Inactive Publication Date: 2009-09-30
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nevertheless, with the development of modern livestock and poultry breeding industry and the improvement of people's living standards, the requirements for the speed and

Method used

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  • Preparation method of biological feed additive
  • Preparation method of biological feed additive
  • Preparation method of biological feed additive

Examples

Experimental program
Comparison scheme
Effect test

Example

[0024] Preparation Example 1

[0025] 1. Select three traditional Chinese medicines of pine needles, astragalus, and hawthorn to be mixed with Ginkgo biloba leaves for fermentation, and select the appropriate Chinese medicine and its ratio with Ginkgo biloba leaves and bran.

[0026] 2. The experiment adopts a two-factor completely randomized design, with a control, a total of 10 treatments, repeated 3 times, the experimental design is shown in Table 1.

[0027] Table 1 Two-factor completely random design table

[0028]

[0029] Table 2 Fermentation comparison of different Chinese medicine-ginkgo biloba combinations

[0030]

[0031] Note: Different lowercase letters indicate significant differences (P<0.05), and different capital letters indicate extremely significant differences (P<0.01).

[0032] 3. The test results show that the fermentation effect of using astragalus-ginkgo biloba-bran as the fermentation raw material is better (Table 2). The fermented product particles o...

Example

[0036] Preparation Example 2

[0037] 1. Transfer the Candida Ruanan strain to the slant medium and culture at 28°C for 20 hours. The above slant medium is a PDA medium. The preparation process is as follows: 200g potatoes are boiled in water and 20g sucrose and 10g agar are added. , Mix well, then add water to make the volume up to 1000mL.

[0038] 2. The Candida utilis obtained in step (1) was inoculated into the liquid seed culture medium on a clean bench, and cultured on a shaker (28° C., 200 rpm) for 24 hours. The preparation process of the liquid seed culture medium is as follows: Dissolve 20 g of glucose, 10 g of peptone, and 10 g of yeast extract in water, and then dilute to 1000 mL with water. The liquid medium was sterilized at 121°C for 20 minutes. The content of yeast in the obtained seed culture solution is 9×10 9 Pieces / mL, store in a refrigerator at 4°C, which can be stored for 1 month.

[0039] 3. Dry and grind astragalus, ginkgo biloba, and bran into fine powder, ...

Example

[0044] Preparation Example 3

[0045] 1. Transfer the Candida Ruanan strain to the slant medium and culture for 24 hours at 25°C. The above slant medium is a PDA medium. The preparation process is as follows: Cut 200g potatoes into small pieces and boil them in water Filter the potato juice with double gauze for 20-30 minutes, add 15g of sucrose and stir to dissolve, then add 20g of agar, continue heating, make the agar completely melt and become transparent, and finally add water to make the volume to 1000mL.

[0046] 2. The Candida utilis obtained in step (1) was inoculated into the liquid seed culture medium on the ultra-clean workbench, and cultured on a shaker (25° C., 200 rpm) for 30 hours. The liquid seed culture medium formula is: 20g glucose, 6g peptone, 10g yeast extract, dissolved in distilled water to a constant volume to 1000mL. The liquid medium was sterilized at 121°C for 20 minutes. The content of yeast in the obtained seed culture solution is 6×10 9 Pieces / mL, put...

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Abstract

The invention relates to a preparation method of biological feed additive which is used for livestock and poultry breeding and can effectively enhance the breeding speed and quality. The preparation method of the biological feed additive is as follows: pulverizing milk veteh, ginkgo leaf and bran into powder, evenly mixing the powder with nutrient fluid to prepare solid culture medium, and fermenting the mixture by Candida utilis to obtain the biological feed additive, wherein the mass ratio of the milk veteh, the ginkgo leaf and the bran is 1:2-4:1; and the preparation method of the nutrient fluid is as follows: dissolving 2.47-3g of cane sugar, 3.29-4g of NH42SO4, 2g of KH2PO4 and 0.5g of MgSO4.7H2O into water and metering the volume to be 100mL with water. The biological feed additive prepared has high nutrition, is rich in amylase, vitamins, minerals, digestive enzyme, somatomedin and relatively complete amino acid, has good palatability and can effectively enhance the breeding speed and quality.

Description

technical field [0001] The invention relates to a preparation method of a biological feed additive, which belongs to the field of animal feed additives. Background technique [0002] Since ancient times in my country, there has been a record of mixing traditional Chinese medicine into feed to treat diseases, and there are tens of thousands of clinically proven effective prescriptions. Chinese herbal medicine is a natural medicine, which can promote the growth and development of livestock and poultry, improve the immune function of the body, and prevent and treat diseases. It also has the advantages of wide source of medicine, low price, good effect, no side effects, and no residue. Astragalus membranaceus is mild in nature, sweet in taste, and has the effects of invigorating qi and solidifying the surface, antiperspirant and detoxification, promoting muscle growth, diuresis, and reducing swelling. The chemical components of Astragalus mainly include flavonoids, saponins and...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K10/12A23K20/147A23K20/163A23K40/00
Inventor 曹福亮金丽杨小虎
Owner NANJING FORESTRY UNIV
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