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Quality control method of hyperthyroidism resistance powder

A quality control method, thin-layer chromatography technology, applied in the field of traditional Chinese medicine, can solve the problems that the quality control method cannot meet the requirements, and achieve the effect of ensuring accuracy and advancement

Active Publication Date: 2009-10-28
JILIN AODONG GRP LIYUAN PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides a quality control method of Yikang powder to solve the problem that the current quality control method cannot meet the requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Determining the content of one component of the drug by high performance liquid chromatography; identifying four components therein by thin layer chromatography;

[0066] Content determination:

[0067] Determination of hesperidin C in the drug by high performance liquid chromatography 28 h 34 o 15 content.

[0068] Chromatographic conditions:

[0069] Octadecylsilane bonded silica gel is used as filler; methanol-water-glacial acetic acid 15:45:0.05 is used as mobile phase; the detection wavelength is 284±2nm, and the number of theoretical plates shall not be less than 3000 based on hesperidin;

[0070] Preparation of reference solution:

[0071] Accurately weigh the appropriate amount of hesperidin reference substance, add methanol to make a solution containing 0.1 mg of hesperidin per 1 ml;

[0072] Preparation of the test solution:

[0073] Accurately weigh 1g of the drug that has been finely ground, put it in a conical flask, accurately add 50ml of methanol, ...

Embodiment approach

[0087] One embodiment of the present invention is: developing agent is n-hexane-chloroform-methanol-diethylamine (5-18: 2-10: 0.5-2: 0.05-1) or n-hexane-chloroform-methanol- Concentrated ammonia test solution (5-18: 2-10: 0.5-2: 0.05-1); and other common developing agents and their proportions.

[0088] b) identify the Ligustrum lucidum composition wherein with thin-layer chromatography:

[0089] Preparation of the test solution:

[0090] Take by weighing 1g of the drug, add 20ml of methanol, heat and reflux for 40 minutes, filter, evaporate the filtrate to dryness, add 0.5ml of the mixed solution of absolute ethanol:chloroform=2:1 to dissolve the residue, and use it as the test solution;

[0091] Preparation of reference solution:

[0092] Weigh the oleanolic acid reference substance, add methanol to make a solution containing 0.5mg of oleanolic acid per 1ml, as the reference substance solution;

[0093] TLC test:

[0094] Take 5 μl of the test solution and 1 μl of the re...

Embodiment 2

[0118] Determining the content of one component of the drug by high performance liquid chromatography; identifying four components therein by thin layer chromatography;

[0119] Content determination:

[0120] Determination of hesperidin C in the drug by high performance liquid chromatography 28 h 34 o 15 content.

[0121] Chromatographic conditions:

[0122] Octadecylsilane bonded silica gel is used as filler; methanol-water-glacial acetic acid 40:56:4 is used as mobile phase; the detection wavelength is 284±2nm, and the number of theoretical plates shall not be less than 3000 based on hesperidin;

[0123] Preparation of reference solution:

[0124] Accurately weigh the appropriate amount of hesperidin reference substance, add methanol to make a solution containing 0.2mg of hesperidin per 1ml;

[0125] Preparation of the test solution:

[0126] Accurately weigh 1 g of the drug that has been finely ground, put it in a conical flask, accurately add 50ml of methanol, weig...

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PUM

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Abstract

The invention relates to a content determination and identification method of hyperthyroidism resistance powder, which pertains to the technical field of traditional Chinese medicine and particularly relates to measurement and identification method of component content in Chinese medicine preparation. The quality control method comprises the methods of: measuring the content of hesperidin with the chemical formula of C28H34O15 in a medicament by a high performance liquid chromatography (HPLC) method and identifying the following components such as corydalis ambigua, fructus ligustri lucidi, alpha-cyperone and paeoniflorin in the medicament by a thin-layer chromatography (TLC) method. The content determination and identification method for hyperthyroidism resistance powder has the advantages of overcoming the shortage of traditional Chinese medicine, establishing an advanced quality control method for the medicament, utilizing the HPLC method to measure the content of the hesperidin in the medicament and conducting TLC identification on the main components such as the corydalis ambigua, the fructus ligustri lucidi, the alpha-cyperone and the paeoniflorin and the like in the medicament, thus ensuring the accuracy and advancement of the medicament content determination and identification method.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a method for measuring and identifying components in traditional Chinese medicine preparations. Background technique [0002] Yikang powder is composed of 40.9g of antelope horn, 62g of white peony root, 82g of Tianzhuhuang, 205g of mulberry, 83g of corydalis (vinegar-roasted), 124g of green skin (vinegar-roasted), 41g of Cyperus cyperi, 39g of scrophulariaceae, 43g of Cassia radix, and 59g of Polygonatum. 144g of chrysanthemum, 144g of asparagus, 121g of asparagus, 1083g of ligustrum lucidum, and 16g of rehmannia glutinosa, and the antelope horn file is ground into fine powder; ), Shi Cassia, and Scrophulariaceae are crushed into fine powder; Xanthogonia, Asparagus, Rehmannia, Ligustrum lucidum, and Polygonatum are decocted twice, adding 8 times the amount of water and decocting for 2 hours for the first time, and adding 6 times the amount for the second ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61K9/14A61P5/16G01N30/36G01N30/90A61K35/56A61K35/32A61K35/618
Inventor 李宏许家洁刘同彦夏国凤陈志国李萍刘召辉柳洪峰曹延梅袁家升
Owner JILIN AODONG GRP LIYUAN PHARM CO LTD
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