Unlock instant, AI-driven research and patent intelligence for your innovation.

Improved extraction method for tylosin

A technology of tylosin and extraction method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high loss of tylosin, low product yield, high impurity content, etc.

Active Publication Date: 2012-03-21
荷本(北京)大药厂有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The experiment of the present invention shows: 1, when simply extracting tylosin with chloroform, the extraction rate is the highest, but the impurity content is also the most in the chloroform extract, which brings difficulties to purification, and the purification process tylosin The loss is more, and the product yield is lower than that of co-solvent extraction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Example 1. Using butyl acetate (BA) to extract tylosin (TL) from fermentation filtrate

[0022] This extraction method is the tylosin extraction process used in the current factory. The extraction process is as follows:

[0023] The fermentation filtrate, pH 6.21, with a titer of 6500-6800 μg / ml, was adjusted to pH 8.41 with 40% NaOH, and BA was added to stir to extract TL. Dosage per extraction: fermentation filtrate / BA=100 / 30 (volume / volume, v / v)

[0024] Results: The residual titer of the aqueous phase after the first extraction: 1150μg / ml, the extraction rate of tylosin: 83.05%;

[0025] The residual titer of the aqueous phase after the second extraction: 521μg / ml, the total extraction rate of the two times: 92.32%.

example 2

[0026] Example 2: Extraction of Tylosin (TL) with 3.85% Trichloromethane (TT) / Butyl Acetate (BA)

[0027] (1) Fermentation filtrate for experiment, with a titer of 6500-6800μg / ml and a pH of 6.21. Take 200ml fermentation filtrate and use saturated Na 2 CO 3 Adjust the pH of the aqueous solution from 6.90 to 6.98 and place it in a 35°C water bath to increase the temperature of the fermentation filtrate to 30 to 33°C.

[0028] (2) Take it out at room temperature and under stirring conditions, adjust the pH to 8.2-8.5.

[0029] (3) Pour into a 250ml separatory funnel, add 8ml TT / BA (3:1), mix well and let stand for about 2 hours, completely layer, release the lower layer, and then use 8ml TT / BA (3:1) for the upper layer Perform a second extraction.

[0030] (4) Combine the extracts of the lower layer twice, centrifuge, wash the precipitate with a small amount of TT, centrifuge, pour the supernatant and combine the extract after centrifugation, and dehydrate with anhydrous sodium sulfate...

example 3

[0037] Example 3. Extract TL with 5.7% TT / BA (3:1)

[0038] (1) Fermentation filtrate, pH 6.37, adjust the pH to 6.98, and raise the temperature of the fermentation broth to 30°C in a 35°C water bath.

[0039] (2) Take 200ml, 30℃, pH 6.98 fermentation filtrate, and use saturated Na 2 CO 3 The pH of the aqueous solution was adjusted to 8.44.

[0040] (3) In a 250ml separatory funnel, add 12ml TT / BA (3:1), mix well for extraction, stand for 2.5 hours, completely separate, take 0.5ml upper layer, add 4.5ml of water, and analyze by HPLC.

[0041] (4) The pH of the upper aqueous phase is 7.97, and 12ml TT / BA (3:1) is added for the second extraction.

[0042] (5) Take 200ml of new fermentation filtrate, adjust the pH to 6.9, heat it to 30°C (pH 6.70) of the fermentation broth at 35°C, and use saturated Na 2 CO 3 (About 5ml) adjust the pH to 8.67, add the second extraction ester phase liquid in item (4) to carry out the first extraction of this batch (using the second extraction solvent).

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
extraction efficiencyaaaaaaaaaa
extraction efficiencyaaaaaaaaaa
extraction efficiencyaaaaaaaaaa
Login to View More

Abstract

The invention relates to an improved extraction method for tylosin. The invention describes the process of using a cosolvent to extract the tylosin from tylosin fermentation filter liquid. The cosolvent comprises chloroform or methylene dichloride and ethyl acetate or butyl acetate or amyl acetic ester or isoamyl acetate or 4-methyl-2-pentanone or toluene. The method using the cosolvent to extractthe the tylosin from the fermentation filter liquid has the advantages that the use quantity of the cosolvent is only 1-8% of the quantity of the fermentation filter liquid; the extraction rate is high and can reach 98.8%; and the cosolvent has a selective extraction function, the impurity content of extraction liquid is very little, product purification is convenient and the product purity is improved.

Description

Technical field [0001] The present invention relates to antibiotic extraction technology. Specifically, it relates to the extraction technology of tylosin, which is a method for extracting tylosin from tylosin fermentation filtrate with a co-solvent. Background technique [0002] Tylosin is a special antibiotic for livestock and poultry, which is a fermentation product of Streptomyces freundii. At present, liquid deep fermentation is adopted at home and abroad. After the fermentation broth is treated with a flocculant (such as aluminum sulfate or aluminum chloride), the fermentation filtrate is obtained by solid-liquid separation. The fermentation filtrate is subjected to liquid-liquid extraction with butyl acetate to obtain the ester phase liquid , The ester phase liquid can obtain tartaric acid or tylosin phosphate through the process of back extraction, concentration and spray drying. [0003] Using butyl acetate to extract tylosin from the fermentation filtrate has two shortc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06C07H17/08
Inventor 王玉万潘真德戴晓曦
Owner 荷本(北京)大药厂有限公司