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Method for enriching ginkgetin compound

A technology of ginkgo flavonoids and compounds, applied in the field of extraction and purification of ginkgo flavonoids, to achieve the effects of environmental protection, high purity, and high purification efficiency

Inactive Publication Date: 2009-12-09
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for enriching ginkgo flavonoids against the shortcomings of the existing methods for extracting and purifying ginkgo flavonoids, which does not require the use of organic solvents such as toluene, methyl ethyl ketone, ethyl butyl ketone, etc., and the cost is relatively low. Low cost, simple operation, high product purity, high extraction and purification efficiency, no "three wastes" discharge, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for enriching ginkgo brass compounds, the specific method steps are as follows:

[0027] (1) prepare ginkgo biloba solution

[0028] Taking Ginkgo biloba powder (i.e. dried and pulverized Ginkgo biloba) as raw material, according to the mass (g) of Ginkgo biloba powder: the ethanol-water solution volume (mL) ratio of 50% ethanol volume fraction is the ratio of 1: 40, in The ginkgo leaf powder is added with an ethanol aqueous solution with a volume fraction of 50%, stirred and dissolved for 30 minutes, and the ginkgo leaf solution is prepared, and the solution is a crude extract solution for dissolving ginkgo flavonoids.

[0029] (2) Centrifugal separation

[0030] After the first step (1) was completed, the ginkgo biloba solution prepared by the (1) step was placed in a centrifuge, and the rotating speed was 3000 rpm, and it was centrifuged for 10 minutes to collect the centrifuged supernatant and lower sediment respectively. For the supernatant, the ginkgo f...

Embodiment 2

[0038] A method for enriching ginkgo flavonoids, the specific method steps are as follows:

[0039] (1) prepare ginkgo biloba solution

[0040] With embodiment 1, wherein: the mass (g) of Ginkgo biloba powder: the ratio of the ethanol-water solution volume (mL) of 50% ethanol volume fraction is 1: 45, stirring and dissolving 45 minutes.

[0041] (2) Centrifugal separation

[0042] With embodiment 1, wherein: the rotating speed of centrifuge is 4500 rev / mins, centrifugal 15 minutes.

[0043] (3) Ultrafiltration separation

[0044] Same as Example 1, wherein: the supernatant in the ultrafilter is separated by ultrafiltration at 0.2MPa, and the filtrate in the rotary evaporator is subjected to a temperature of 70°C and a vacuum of -0.7MPa. evaporation.

[0045] (4) Electrodialysis

[0046] Same as Example 1, wherein: for the ethanol-removing solution in the electrodialyzer, first adjust its pH value to 7.0 with dilute sodium hydroxide solution, and perform electrodialysis at...

Embodiment 3

[0050] A method for enriching ginkgo flavonoids, the specific method steps are as follows:

[0051] (1) prepare ginkgo biloba solution

[0052] With embodiment 1, wherein: the mass (g) of Ginkgo biloba powder: the ratio of ethanol-water solution volume (mL) of 50% ethanol volume fraction is 1: 60, stirring and dissolving 60 minutes.

[0053] (2) Centrifugal separation

[0054] With embodiment 1, wherein: the rotating speed of centrifuge is 6000 rev / mins, centrifugal 20 minutes.

[0055] (3) Ultrafiltration separation

[0056] Same as Example 1, wherein: the supernatant in the ultrafilter is subjected to ultrafiltration separation at 0.3 MPa. Evaporate the filtrate in the rotary evaporator at a temperature of 80° C. and a vacuum of -0.8 MPa.

[0057] (4) Electrodialysis

[0058] Same as Example 1, wherein: for the ethanol removal solution in the electrodialyzer, first adjust its pH value to 7.5 with dilute sodium hydroxide solution, and perform electrodialysis at a voltage...

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PUM

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Abstract

The invention discloses a method for enriching a ginkgetin compound, which relates to a method for extracting a purified flavone compound from folium ginkgo. The method takes folium ginkgo powder as a raw material to prepare freeze-dried powder of the ginkgetin compound by dissolution, centrifugal and ultrafiltration separation, electrodialysis, concentration and freeze drying. The method has simple equipment, simple and convenient operation and easy control, so the production is safe and the cost is low. A product prepared by the method has high purification rate and good quality; and byproducts produced during the production such as removed foreign protein, a centrifugal precipitate as well as used ethanol and pure water can be recovered and utilized and are not discharged, so no 'three wastes' pollute the environment, the resources are fully utilized and production cost is further reduced. The product prepared by the method can be widely applied to medicament, health care product and cosmetic industries.

Description

1. Technical field [0001] The invention belongs to the technical field of extracting and purifying ginkgo flavonoids, in particular to a method for extracting and purifying ginkgo flavonoids from ginkgo leaves. 2. Background technology [0002] Ginkgo biloba is known as the "living fossil" of the plant kingdom, and it is mainly distributed in China, Korea, Japan and other countries. Scientific research has found that Ginkgo biloba contains a large number of polar and non-polar compounds, mainly flavonoids, terpenoid lactones, organic acids, alkylphenols and alkylphenolic acids, steroids and trace elements. Modern pharmacological studies have shown that ginkgo flavonoids have the effects of dilating blood vessels, lowering serum cholesterol, antispasmodic, antibacterial and relaxing bronchi, and can capture free radicals and inhibit platelet activating factors. The preparation prepared from Ginkgo biloba extract has therapeutic value for diseases such as cardiovascular and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P39/06A61P21/02A61P1/06A61P1/10A61P31/04A61P31/12A61P5/30A61P29/00A61P1/16A61P9/00A61K8/97A61K127/00
Inventor 桑鲁燕周小华云芳张桂罗
Owner CHONGQING UNIV