Culture medium suitable for industrial production of bifidobacteria and preparation method thereof
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A bifidobacterium and culture medium technology, applied in the field of bifidobacterium culture medium and its preparation, can solve the problems of cumbersome operation, intolerance to freezing, and harmful effects of inorganic salt components on human and animal health, and achieve simple production process Effect
Active Publication Date: 2009-12-09
江西舒美特药业有限公司
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Problems solved by technology
TPY and PTYG media have weak buffering capacity and are not suitable for large-scale use; MRS and NPNL are rich in nutrients and have strong buffering capacity, but the current MRS and NPNL media have the following problems: high price (NPNL 13 yuan/L, MRS 3 Yuan/L; phosphate in the buffer system can easily cause Lactobacillus to change from a short rod resistant to freezing to a fibrous rod that is not resistant to freezing; more inorganic salts have potential harmful effects on human and animal health; L-cysteine salt Problems such as the unpleasant smel
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[0041] Example 1:
[0042] (1) Preparation of corn syrup: After weighing the dry corn syrup, add water to 55~63g / L, filter with gauze, and set aside;
[0045] (4) Sterilize at 110℃~121℃ for 10~15min for later use.
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[0046] Example 2:
[0047] (1) Preparation of corn syrup: select the refined corn syrup powder that has been spray-dried, and after weighing, add water to 63g / L, adjust the pH to 7.2, filter with 8 layers of gauze, and set aside;
[0048] (2) Add glucose, 10g / L;
[0049] (3) Adjust the pH value to 6.5;
[0050] (4) Divide, sterilize at 110℃~121℃ for 10~15min for later use.
Example Embodiment
[0051] Example 3:
[0052] (1) Preparation of corn syrup: select the refined corn syrup powder that has been spray-dried, and after weighing, make up water to 58g / L, adjust the pH to 7.2, filter with 8 layers of gauze, and set aside;
[0055] (4) Divide, sterilize at 110℃~121℃ for 10~15min for later use.
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Abstract
The invention discloses a culture medium suitable for industrial production of bifidobacteria and a preparation method thereof. The culture medium consists of the following components: 55 to 63 grams per liter of corn steep liquor dry powder (containing 40 percent of protein), 5 to 10 grams per liter of glucose, and 0 to 1 gram per liter of tween-80, wherein the initial pH is between 6.5 and 7.2.The bifidobacteria BBMN68 and BB12 grow well in the culture medium, and the maximum amount of growth reaches 10<9> cfu/ml which is better than that of in the bifidobacteria culture media MRS and NPNL. The culture medium has low cost (the price is reduced by 95 percent compared with that of the NPNL culture medium and is reduced by 80 percent compared with that of the MRS culture medium) and simple preparation process, and is suitable for industrial mass production of the bifidobacteria.
Description
technical field [0001] The invention relates to a culture medium of bifidobacteria and a preparation method thereof, in particular to a culture medium of bifidobacteria suitable for industrialization. . Background technique [0002] Bifidobacterium (Bifidobacterium) is one of the important physiological bacteria in the intestinal tract of warm-blooded animals such as humans and animals, and one of the important indicators of human health. Its proportion in the intestinal flora directly affects the health of the host. Other normal flora together form the colonization resistance to prevent the invasion of foreign bacteria, and have a certain biological antagonism. At the same time, bifidobacteria contain a large number of digestive enzymes, which can digest some nutrients that cannot be digested by their own enzyme system in the body, and promote The absorption of nutrients in the body, bifidobacteria and their surface structure components can be used as biological responders...
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