Preparation method of chitosan asymetric membrane for leading periodontium to be regenerated

A periodontal tissue regeneration and asymmetric membrane technology, which is applied in the field of preparation of chitosan asymmetric membranes, can solve the problems of expensive periodontal tissue regeneration materials, blank periodontal regeneration materials, and influence on the quality of osteogenesis, and achieves good Osteoinductive, low cost, good histocompatibility

Inactive Publication Date: 2009-12-23
ZHEJIANG UNIV
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because in the process of alveolar bone regeneration, fibroblasts only need one week to fill the defect area, while osteoblasts need 3 months. If the growth of fibroblasts is not interrupted, the bone defect area will be filled with fibroblasts. Instead of osteoblasts grow in, thus affecting the quality of bone formation, so an isolation membrane is needed to isolate the body tissue from the alveolar bone
At present, no...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity average molecular weight of 200,000 to a 2% acetic acid solution, and stir vigorously for 2 hours to obtain a 3% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, and after being treated with sodium hydroxide coagulation solution, it was dried at 50°C to obtain a chitosan matrix film with a thickness of 15 μm.

[0015] 2) Preparation of spinning solution A: dissolving chitosan powder with a viscosity average molecular weight of 200,000 in an acetic acid solution with a volume fraction of 70%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 60:40, a mixed solution with a total concentration of 7% is prepared, and the spinning solut...

Embodiment 2

[0019] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity-average molecular weight of 560,000 to a 2% acetic acid solution and stir vigorously for 1 hour to obtain a 5% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, which was treated with sodium hydroxide coagulation solution and dried at 70° C. to obtain a chitosan matrix film with a thickness of 30 μm.

[0020] 2) Preparation of spinning solution A: Dissolve chitosan powder with a viscosity average molecular weight of 560,000 in an acetic acid solution with a volume fraction of 80%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 80:20, a mixed solution with a total concentration of 10% is prepared, and the spinning solution A is ob...

Embodiment 3

[0024] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity average molecular weight of 750,000 to a 2% acetic acid solution and stir vigorously for 2 hours to obtain a 6% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, and after being treated with sodium hydroxide coagulation solution, it was dried at 80°C to obtain a chitosan matrix film with a thickness of 40 μm.

[0025] 2) Preparation of spinning solution A: Dissolve chitosan powder with a viscosity average molecular weight of 750,000 in an acetic acid solution with a volume fraction of 90%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 90:10, a mixed solution with a total concentration of 5% is prepared, and the spinning solution...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Thicknessaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method of chitosan asymetric membrane for leading periodontium to be regenerated, comprising the steps of: preparing basal plasmamembrane of the chitosan by means of forming a salivating membrane; preparing chitosan/polyvinyl alcohol spinning solution A and hydroxylapatite/chitosan/ alcohol spinning solution B; filling the spinning solution A or the spinning solution B into an injector; and eclectically spinning a layer of nonwoven membrane with porous structure on the basal plasmamembrane of the chitosan by means of spinning anelectric. The chitosan asymetric membrane can provide more structural space for cell growth and good microenvironment for cell survival, and can actively lead the periodontium to be regenerated (the regeneration comprises agglutination, differentiation, proliferation and secretion of extracellular matrix). Meanwhile, the basal plasmamembrane of the chitosan also can be used as a barrier membrane to stop the ingeneration of fibroblast, and is good for the osteoblast to grow into a defect area of the periodontium. The preparation method is simple, has low cost and good tissue compatibility, and can be adsorbed in human body.

Description

Technical field [0001] The invention relates to a preparation method of chitosan asymmetric membrane for guiding regeneration of periodontal tissue. Background technique [0002] In periodontal treatment and dental implant surgery, how to ensure that periodontal ligament cells preferentially occupy the root surface is the key to today's periodontal tissue regeneration (GTR) technology. Because in the regeneration process of alveolar bone, it only takes one week for fibroblasts to fill the defect area, while osteoblasts need 3 months. If the growth of fibroblasts is not blocked, the bone defect area will be filled with fibroblasts. Instead of the osteoblasts growing in, which affects the quality of bone formation, an isolation membrane is needed to isolate the body tissue from the alveolar bone. Currently, the most widely used clinically non-absorbable GTR material, although it has good histocompatibility, it cannot be degraded and requires a second operation to remove it. The pri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L31/12A61L31/16A61L31/04A61L31/02
Inventor 胡巧玲王征科张凯
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap