Method for efficiently saccharifying straw pulp fiber

A fiber-like and straw-pulp technology, applied in the field of high-efficiency saccharification of straw-like fibers, can solve the problems of equipment corrosion engineering cost, increase production cost, and high energy consumption of mechanical treatment methods, achieve small equipment investment, improve raw material yield, reduce The effect of degradation loss

Active Publication Date: 2009-12-30
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem is that the energy consumption of the mechanical treatment method is very high, which undoubtedly increases the production cost
[0017] Acid hydrolysis has a history of nearly 100 years. Up to now, there are still many problems, such as acid recovery, equipment corrosion, project cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In a suitable reactor, add 400 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution at a solid-to-liquid ratio of 1:7 (weight ratio), the concentration of lye is 2%, and seal the mouth. Then, it was pretreated by heating at 85°C for 1.5 hours, the obtained slurry was centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor was treated with a membrane to recover the alkali in it; the obtained solid phase was put into a container, and water was added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50°C, add cellulase to the feed liquid, the amount of enzyme used is 25FPIU / g substrate (on dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, sampling once every 24 hours, using DNS method to measure the total sugar concentration in the enzymatic hydrolysis solution, and using high performance liquid chromatography to measure the glucose and xylose ...

Embodiment 2

[0038] In a suitable reactor, add 400 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution at a solid-to-liquid ratio of 1:7 (weight ratio), the concentration of lye is 2%, and seal the mouth. Then, it was pretreated by heating at 90°C for 1.5 hours, the obtained slurry was centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor was treated with a membrane to recover the alkali in it; the obtained solid phase was put into a container, and water was added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50°C, add cellulase to the feed liquid, the amount of enzyme used is 25FPIU / g substrate (on dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, sampling once every 24 hours, using DNS method to measure the total sugar concentration in the enzymatic hydrolysis solution, and using high performance liquid chromatography to measure the glucose and xylose co...

Embodiment 3

[0040] In a suitable reactor, add 1200 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution in a ratio of 1:7 (weight ratio) to solid-to-liquid ratio, the concentration of lye is 2%, and seal the mouth. Then, it was pretreated by heating at 95°C for 1.5 hours, the obtained slurry was centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor was treated with a membrane to recover the alkali therein; the obtained solid phase was added to a container, and water was added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50°C, add cellulase to the feed liquid, the amount of enzyme used is 25FPIU / g substrate (on dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, sampling once every 24 hours, using DNS method to measure the total sugar concentration in the enzymatic hydrolysis solution, and using high performance liquid chromatography to measure the glucose...

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Abstract

The invention relates to a method for efficiently saccharifying straw pulp fiber, comprising pre-treatment of straw pulp fiber and enzymolysis, wherein in the pre-treatment process, straw pulp fiber and aqueous alkali at a concentration of 0.1wt%-50wt% are mixed to be treated at the temperature of 70 DEG C-140 DEG C for 10-400 min; the weight ratio of the straw pulp fiber to the aqueous alkali is1:1-20.The method for efficiently saccharifying straw pulp fiber of the invention produces no harmful substance to enzymolysis and fermentation, reduces degradation loss of carbohydrate in biomass, improves raw material enzymolysis yield and effectively lowers production cost.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a method for efficiently saccharifying grass pulp fibers. Background technique [0002] Oil resources, which are the backbone of the world economy, are expected to be depleted in about a few decades. Therefore, the development and research of oil substitutes is imminent. At present, many countries are studying the preparation of fuel ethanol by biotransformation using wood biomass resources as raw materials to replace or partially replace oil with limited reserves. [0003] The main components of woody biomass are cellulose, hemicellulose and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% (the absolute dry weight of the cellulose raw material). It is a very mature process to produce ethanol from hexose by fermentation of Saccharomyces cerevisiae. When using cellulase to hydrolyze woody biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 李荣杰薛培俭尚海涛黄之文
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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