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Luteinizing hormone time-resolved fluoroimmunoassay and kit

A luteinizing hormone and immunofluorescence technology, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve the problems of polluted environment, low sensitivity, cumbersome operation, etc., and achieve small cross-reaction, good specificity, and short detection time short effect

Inactive Publication Date: 2009-12-30
PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a time-resolved immunofluorescence analysis method and kit for LH detection, which mainly solve the technical problems of low sensitivity, poor stability, cumbersome operation and environmental pollution in the prior art.

Method used

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  • Luteinizing hormone time-resolved fluoroimmunoassay and kit
  • Luteinizing hormone time-resolved fluoroimmunoassay and kit
  • Luteinizing hormone time-resolved fluoroimmunoassay and kit

Examples

Experimental program
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Effect test

Embodiment

[0032] Example: Luteinizing hormone (LH) assay

[0033] 1. Preparation of standard products

[0034] Use 6% BSA, 4% sugar, 0.1% NaN3, 50mmol / L Tris-HCl buffer solution to prepare LH protein into 0, 3, 10, 30, 100, 300mIU / mL standard solution, and calibrate with national LH standard. Aliquot and store at +2~+8℃.

[0035] 2. Operation steps

[0036] 1) Preparation of reagents

[0037] a) Washing liquid: Mix 40mL of concentrated washing liquid and 960mL of deionized water in a clean washing liquid bottle, and use it as a working washing liquid for later use.

[0038] b) Europium labeling: within one hour before use, dilute the europium label 1:20 with assay buffer.

[0039] c) Equilibrate the kit analysis buffer, the sample to be tested, the calibrator and the required number of microwell reaction strips to room temperature (20-25° C.).

[0040] 2) Pipette 25 μl of calibrator or sample, and add to the bottom of the corresponding microwell in sequence.

[0041] 3) Add 100 μl...

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Abstract

The invention discloses luteinizing hormone (LH) time-resolved fluoroimmunoassay and a kit. In the luteinizing hormone (LH) time-resolved fluoroimmunoassay, an anti-LH monoclonal antibody is used as a coating antibody and diluted by a sodium carbonate buffer solution to 1-10 ug / ml to serve as a coating solution, a lanthanide ion label paired with the anti-LH monoclonal antibody serves as a label antibody and is diluted by reaction buffer solution in a ratio of 1:20 for use during experiment, and 25mu liters of an LH standard product or a sample to be detected as well as the diluted label antibody is filled in holes on a reaction plate coated by the antibody sequentially for fluorescence detection after incubation. The invention also provides the corresponding kit. The kit has high sensitivity, specificity and stability; and the luteinizing hormone (LH) time-resolved fluoroimmunoassay adopts a high automatic analysis system and can improve clinic detection result generation speed and greatly reduce human errors and improve the reliability of the detection result.

Description

Technical field: [0001] The invention relates to a luteinizing hormone (LH) quantitative detection kit, which belongs to the detection field of time-resolved immunofluorescence analysis. Background technique: [0002] Luteinizing Hormone (LH) is a glycoprotein secreted by the anterior pituitary gland, composed of two polypeptide chains bound by non-covalent bonds. The secretion of LH is mainly regulated by GnRH in the hypothalamus, and it is also affected by the menstrual cycle. Its physiological function is mainly to promote female ovulation and corpus luteum production, so as to promote the secretion of estrogen and progesterone by the corpus luteum. In men, it mainly promotes the proliferation of testicular interstitial cells, and promotes the synthesis and secretion of testosterone. [0003] Currently, serum LH detection mainly includes enzyme-linked immunoassay (ELISA) and immunoradiological assay (IRMA). Due to the limitation of tracer properties, the sensitivity of...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N21/64
Inventor 沈君吴冯波
Owner PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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