Extraction method of prolease in swine stomach

An extraction method and protease technology, which are applied in the field of extraction of porcine pepsin, can solve the problems of low extraction rate and long time consumption, and achieve the effects of reducing extraction time and improving the activity of the enzyme.

Inactive Publication Date: 2010-01-13
张丽萍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this process takes a long time

Method used

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  • Extraction method of prolease in swine stomach
  • Extraction method of prolease in swine stomach
  • Extraction method of prolease in swine stomach

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Below in conjunction with experiment the present invention will be further described:

[0015] Experimental Materials:

[0016] Pig gastric mucosa.

[0017] Reagent:

[0018] The main reagents used in the experiment are listed in Table 1.

[0019] Main Reagent Table 1

[0020] serial number

Reagent name

Specification

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Standard Bovine Serum Albumin (BSA)

Sodium chloride

Sodium Hydrogen Phosphate

Potassium dihydrogen phosphate

Acetic acid

sodium hydroxide

ammonium sulfate

Anhydrous Sodium Carbonate

Sodium Tungstate

copper sulfate

Sodium molybdate

EDTA

barium chloride

Concentrated hydrochloric acid

phosphoric acid

Silver nitrate

Absolute ethanol

tr...

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Abstract

The invention relates to an extraction method of prolease in swine stomach, comprising the following steps: selecting the stomach of an adult swine; picking off the mucosa at the bottom part of the fundus of the swine stomach with thickness of about 2-3 mm and mincing; adding hydrochloricacidextract of 1:2-4 times with the pH value of 2.5 into the minced mucosa; adding tween-80 with the concentration of 5 mg/mL-15 mg/mL; placing the above mixed liquor into an ultrasonic wave medicine processor for treatment in the icebath by utilizing ultrasonic wave, wherein, the power of the ultrasonic wave is 220+/-20 W-320+/-20 W, the frequency of the ultrasonic wave is 23 kHz-43 kHz, and the treatment time of the ultrasonic wave is 90s-150s; then transferring into the water bath with the temperature of 45 DEG C for digestion for 58 min; centrifugating and extracting supernate to obtain the protease. The highest specific activity of the protease in the swine stomach extracted by the ultrasonic-assistant acid method reaches 5.82 U/mg, while the specific activity by traditional acid digestion is only 3.5 U/mg, and the digestion time is reduced by around 1.5h.

Description

Technical field: [0001] The invention relates to a method for extracting pig pepsin. Background technique: [0002] Pepsin (pepsin) belongs to aspartic acid proteolytic enzymes, is gastric digestion proteolytic enzymes, and its precursor is pepsinogen. Pepsinogen is synthesized in the gastric base mucosa of vertebrates and converted into pepsin under the acidic conditions of gastric juice. At present, the pepsin in the domestic and foreign markets is mainly extracted from the gastric mucosa of pigs, and the molecular weight of pig pepsin (EC 3.4.23.1) is 31-36ku. The extraction method of porcine pepsin in my country is mainly the traditional acid extraction combined with organic solvent separation, the crude enzyme liquid is degreased with ether, concentrated and dried, and the obtained product enzyme activity is only 1948.5U, and the production cycle of this method is long, and the production The technical parameters are unfavorable, and the obtained porcine pepsin activit...

Claims

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Application Information

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IPC IPC(8): C12N9/64
Inventor 张丽萍崔素萍王莹魏文毅贾建刘妍妍李艳青曹荣安杨楠李良玉
Owner 张丽萍
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