Sterilization composite
A technology of composition and fungicides, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of complex bactericidal mechanism of triazole fungicides, interference with developmental growth and spore formation, and unsatisfactory effect when used alone. , to achieve the effect of overcoming disease resistance, reducing production and use costs, and reducing the amount of field drugs
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example 1
[0018] Bioassay example 1 Toxicity determination of cyprodinil (A) and a triazole fungicide (B) compounded against cucumber powdery mildew
[0019] Test object: Cucumber powdery mildew
[0020] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006" This test uses the cucumber powdery mildew pot test method. On the basis of the preliminary test, select 2 melon seedlings at the true leaf stage with the same growth, write the label number with an oily marker, insert it into the pot of cucumber seedlings, discharge them in sequence, and spray the medicine on the melons by spraying the leaves. On the leaves of seedlings, after spraying treatment, dry the test materials in the fume hood or greenhouse for 24 hours before inoculation. Cultivate in the greenhouse for 7 days to investigate the control effect, the grading standard implements the nati...
example 2
[0033] Biological Assay Example 2: Cyprodinil (A) and a triazole fungicide (B) combined toxicity assay for rice blast
[0034] Test object: rice blast fungus
[0035] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006". There were five replicates for each concentration, and a total of 10 rice plants were divided into 5 pots for each treatment. Water treatment was used as blank control. Wash the surface spores of the cultivated pathogenic bacteria with sterile water, and make 1×10 6 spores / ml spore suspension, at the three-leaf stage of rice, use a crop sprayer to inoculate on the rice leaves and evenly spray the inoculation. After 24 hours of darkness, moisturize and remove after 10 days. The number of diseased spots on each rice leaf (30 leaves in total) was counted, and graded according to the number of leaf diseased spots. Calcul...
example 3
[0055] Biological assay example 3: Toxicity determination of cyprodinil (A) and a triazole fungicide (B) compounded against Botrytis cinerea
[0056] Test object: Botrytis cinerea of cucumber, Botrytis cinerea of tomato
[0057] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press) and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006" using the growth rate method (plate method). On the basis of the preliminary test, on the aseptic operation table, quantitatively add the pre-thawed sterilized medium into the sterile Erlenmeyer flask according to the test treatment. Have 45ml of heat medium (PDA medium, 45-50 ℃) in the Erlenmeyer flask, after shaking well, pour into the glass petri dish of diameter 90mm rapidly, each petri dish is poured into 10ml of culture medium with medicine. Set aside horizontally to cool and form into slabs. Each concentration was replicated five times. The treatmen...
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