Integrated microfluidic sensing chip and method for detecting microfluid

Abstract

The invention discloses an integrated microfluidic sensing chip and a method for detecting microfluid. The integrated microfluidic sensing chip comprises a rigid polymer substrate, a polydimethylsiloxane circulating layer and a surface plasma resonance sensing sheet. Tested objects flow through a filter micro column of a filter passage of the polydimethylsiloxane circulating layer to be filtered and separated, and the tested objects which do not pass through the filter micro column enter a waste liquid passage and are discharged from a waste liquid outlet. The tested objects which pass through the filter micro column separation enter a sensing detection passage of the chip to react with specific biological molecules on the surface plasma resonance sensing sheet, and the surface plasma resonance sensing sheet carries out labeling-free and real-time quantitative detection for the biochemical reaction on the chip. The method integrates sample filtration and pretreatment, separation and labeling-free sensing detection, and has the characteristics of high integration degree, simple operation, high detection sensitivity, labeling prevention and little sample consumption. The integrated microfluidic sensing chip and the method are suitable for clinical diagnosis and analysis for body fluid containing complex components such as blood, urine, spit and the like.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic sensor chip and an analysis method integrating sample filtration, separation and label-free sensing and detection. Background technique [0002] Microfluidic chip is a new type of biochemical chip developed in the 1990s on the basis of multi-disciplinary interdisciplinary and multi-technical integration of microelectromechanical system (MEMS) technology, life science, analytical science and information science. analytical skills. Compared with traditional biochemical analysis techniques, microfluidic chips have many advantages such as small size, low reagent consumption, fast analysis speed, automated analysis process, easy integration and high throughput. It has broad application prospects in many fields such as disease diagnosis, public safety, aerospace, and high-throughput drug screening. At present, it has become the most active resear...

AI Technical Summary

Problems solved by technology

Laser-induced fluorescence (laser-induced fluorescence, LIF) is currently the most widely used and most sensitive detection method in microfluidic chip detection, but the detection device has a large volume, complex structure, and poor versatility (only suitable for a small amount of The detection of biological samples labeled with fluorescent dyes) and other problems cannot meet the home and portable requirements of microfluidic chip detection

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