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Integrated microfluidic sensing chip and method for detecting microfluid

A sensor chip and sensor chip technology, which is applied in biological testing, material inspection products, scattering characteristics measurement, etc., can solve the problem that the microfluidic chip detection cannot meet the requirements of home use and portability, and the detection device is large in size and complex in structure. and other problems, to achieve the effect of high integration, fast analysis speed and high sensitivity

Inactive Publication Date: 2010-02-17
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

Laser-induced fluorescence (laser-induced fluorescence, LIF) is currently the most widely used and most sensitive detection method in microfluidic chip detection, but the detection device has a large volume, complex structure, and poor versatility (only suitable for a small amount of The detection of biological samples labeled with fluorescent dyes) and other problems cannot meet the home and portable requirements of microfluidic chip detection

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  • Integrated microfluidic sensing chip and method for detecting microfluid

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[0027] Example: use the microfluidic sensor chip to detect microalbumin (HSA) in urine. Such as figure 1 As shown, the rigid polymer substrate 1 of the microfluidic sensor chip is made of plexiglass to form a 22mm (length)×22mm (width)×15mm (height) plexiglass support base. The surface plasmon resonance sensor 3 is made by depositing a layer of about 2nm chromium on a cover glass with a thickness of 20mm×20mm and a thickness of 170μm by using magnetron sputtering technology, and then depositing a layer of 50nm gold. The competitive immunoassay is used for detection, and the main experimental steps are as follows:

[0028] 1. Chip carboxymethyl dextran modification of the present invention: first use 5×10 -3The 11-mercapto-1-undecanol solution of mol / L soaks the surface plasmon resonance sensing chip 3 and crosses the liquid, improves the hydrophilicity of the gold film surface, then the sensing chip 3 is put into 0.6mol / L epichlorohydrin solution ( The solvent was 0.1 mol / L...

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Abstract

The invention discloses an integrated microfluidic sensing chip and a method for detecting microfluid. The integrated microfluidic sensing chip comprises a rigid polymer substrate, a polydimethylsiloxane circulating layer and a surface plasma resonance sensing sheet. Tested objects flow through a filter micro column of a filter passage of the polydimethylsiloxane circulating layer to be filtered and separated, and the tested objects which do not pass through the filter micro column enter a waste liquid passage and are discharged from a waste liquid outlet. The tested objects which pass through the filter micro column separation enter a sensing detection passage of the chip to react with specific biological molecules on the surface plasma resonance sensing sheet, and the surface plasma resonance sensing sheet carries out labeling-free and real-time quantitative detection for the biochemical reaction on the chip. The method integrates sample filtration and pretreatment, separation and labeling-free sensing detection, and has the characteristics of high integration degree, simple operation, high detection sensitivity, labeling prevention and little sample consumption. The integrated microfluidic sensing chip and the method are suitable for clinical diagnosis and analysis for body fluid containing complex components such as blood, urine, spit and the like.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic sensor chip and an analysis method integrating sample filtration, separation and label-free sensing and detection. Background technique [0002] Microfluidic chip is a new type of biochemical chip developed in the 1990s on the basis of multi-disciplinary interdisciplinary and multi-technical integration of microelectromechanical system (MEMS) technology, life science, analytical science and information science. analytical skills. Compared with traditional biochemical analysis techniques, microfluidic chips have many advantages such as small size, low reagent consumption, fast analysis speed, automated analysis process, easy integration and high throughput. It has broad application prospects in many fields such as disease diagnosis, public safety, aerospace, and high-throughput drug screening. At present, it has become the most active resear...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/50G01N21/55G01N35/08
Inventor 崔大付刘长春陈兴
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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