Microbiological preparation capable of improving soil continuous cropping obstacle and production method thereof
A technology for microbial preparation and continuous cropping barrier, which is applied in the field of agricultural production and application of microorganisms, can solve the problems of difficulty in multiplying and increasing aerobic bacteria, inhibiting bacterial survival, and difficult to remove disease bacteria, so as to facilitate normal respiration and prevent continuous cropping barriers. , the effect of inhibiting soil-borne diseases
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Embodiment 1
[0042] Step 1: Breeding of Fermentation Strains
[0043] a. Bacterial strain screening: select well-growing planting soil to carry out microbial sterilization with conventional methods, add 10 times the weight of sterile water to the sterilized soil and soak for 24 hours, and use photosynthetic bacteria culture medium to screen out red bacteria in the leachate. Pseudomonas, use Azotobacter Medium to screen Azotobacter brownus, use beef extract peptone agar medium (LB) to screen Bacillus subtilis, Methylomonas, use potato sucrose agar (PDA) medium to screen Streptomyces;
[0044] b. Shake flask count screening: the above strains that were screened were diluted to 10% with 0.9% normal saline. -5 -10 -7 The final bacterial suspension was cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate was selected for a shake flask experiment to observe the growth rate of the strains and screen for strains with a quantity higher than 5 ...
Embodiment 2
[0060] Step 1: Breeding of Fermentation Strains
[0061] With embodiment 1;
[0062] Step Two: Liquid Culture of Beneficial Bacteria
[0063] a. each strain is weighed by the following parts by weight: 13 parts of Pseudomonas fluorescens, 23 parts of Azotobacter prototrophans, 33 parts of Bacillus subtilis, 13 parts of Methylomonas, 18 parts of Streptomyces;
[0064] b. Liquid culture of bacterial strains: inoculate the mixed bacterial lawn of the above bacterial strains into the liquid medium sterilized at 125° C. for 40 minutes at a ratio of 1:30, and the rest are the same as in Example 1.
[0065] C, with embodiment 1.
[0066] The parts by weight of the liquid medium are 3 parts of corn steep liquor, 2 parts of barley starch, 0.3 part of ammonium bisulfate, 14 parts of molasses, 2 parts of potassium phosphate, 0.8 part of potassium sulfate, 0.5 part of trace element solution, and 77.4 parts of sterile water; Step: Solid expansion culture of strains
[0067] a. After mi...
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