Microbiological preparation capable of improving soil continuous cropping obstacle and production method thereof

A technology for microbial preparation and continuous cropping barrier, which is applied in the field of agricultural production and application of microorganisms, can solve the problems of difficulty in multiplying and increasing aerobic bacteria, inhibiting bacterial survival, and difficult to remove disease bacteria, so as to facilitate normal respiration and prevent continuous cropping barriers. , the effect of inhibiting soil-borne diseases

Inactive Publication Date: 2010-03-24
SHANGHAI CHUANGBO ECOLOGICAL ENG
View PDF1 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, since the vegetables in the rotation are generally of the same family and genus, the diseases are the same. At the same time, the pathogenic plants of the vegetables and the disease bacteria in the soil are not easy to remove in the greenhouse, and the soil diseases and the alternation of dry and wet in the greenhouse environment are not obvious, so that the soil is in a long-term environment. In an anaerobic environment, it is difficult for beneficial aerobic bacteria to multiply and increase, making it difficult for antagonistic microorganisms in the soil to inhibit bacteria from surviving
[0009] A currently published patent application document titled "Application of White-rot Fungal Agents in Improving Soil Continuous Cropping Obstacles", application number: 200610017969.5, uses a white-rot fungal agent to apply deep in the soil to improve soil structure and inhibit Diseases, due to the use of a single strain of bacteria, it is impossible to effectively inhibit complex diseases in the soil, so the control effect is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Step 1: Breeding of Fermentation Strains

[0043] a. Bacterial strain screening: select well-growing planting soil to carry out microbial sterilization with conventional methods, add 10 times the weight of sterile water to the sterilized soil and soak for 24 hours, and use photosynthetic bacteria culture medium to screen out red bacteria in the leachate. Pseudomonas, use Azotobacter Medium to screen Azotobacter brownus, use beef extract peptone agar medium (LB) to screen Bacillus subtilis, Methylomonas, use potato sucrose agar (PDA) medium to screen Streptomyces;

[0044] b. Shake flask count screening: the above strains that were screened were diluted to 10% with 0.9% normal saline. -5 -10 -7 The final bacterial suspension was cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate was selected for a shake flask experiment to observe the growth rate of the strains and screen for strains with a quantity higher than 5 ...

Embodiment 2

[0060] Step 1: Breeding of Fermentation Strains

[0061] With embodiment 1;

[0062] Step Two: Liquid Culture of Beneficial Bacteria

[0063] a. each strain is weighed by the following parts by weight: 13 parts of Pseudomonas fluorescens, 23 parts of Azotobacter prototrophans, 33 parts of Bacillus subtilis, 13 parts of Methylomonas, 18 parts of Streptomyces;

[0064] b. Liquid culture of bacterial strains: inoculate the mixed bacterial lawn of the above bacterial strains into the liquid medium sterilized at 125° C. for 40 minutes at a ratio of 1:30, and the rest are the same as in Example 1.

[0065] C, with embodiment 1.

[0066] The parts by weight of the liquid medium are 3 parts of corn steep liquor, 2 parts of barley starch, 0.3 part of ammonium bisulfate, 14 parts of molasses, 2 parts of potassium phosphate, 0.8 part of potassium sulfate, 0.5 part of trace element solution, and 77.4 parts of sterile water; Step: Solid expansion culture of strains

[0067] a. After mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a microbiological preparation capable of improving soil continuous cropping obstacle and production method thereof, characterized in that: the object strain is bred using theleach solution of the sampling soil using fermentation technology of liquid high-speed culture and solid amplification culture and the strain is mainly selected from synthetic microbes absent in the greenhouse soil and a stable system is formed in the soil and simultaneously a special substrate carrier ensures the soil permeable and loose and is convenient for the growth of aerobic beneficial microbes and capable of relatively completely solving or preventing soil continuous-cropping puzzle from aspects of soil-borne disease prevention, soil improvement and forpadion of stable beneficial microbes ecology soil system. The microbiological preparation is a novel microbe agricultural technique product.

Description

technical field [0001] The invention relates to a microbial preparation and a preparation method for improving soil continuous cropping obstacles, in particular to a technology and product for improving soil continuous cropping obstacles, which can significantly improve soil aggregate structure, reduce soil-borne diseases, and inhibit the occurrence of soil continuous cropping obstacles, belonging to microorganisms In the field of agricultural production application technology. Background technique [0002] Continuous cropping obstacle refers to the phenomenon of reduced yield, poor quality, aggravated disease, poor growth status, or even failure to grow after continuous planting of the same crop or related crops in the same soil. [0003] Vegetables and melons and fruits are highly intensive crops with high multiple cropping index. Greenhouse cultivation is generally used now. Due to the relatively closed environment, continuous cropping obstacles are very common. Continuou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/00C12R1/39C12R1/065C12R1/125C12R1/26C12R1/465
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products