Method for extracting efficient degrading bacteria of carbamazepine

A technology of high-efficiency degrading bacteria and carbamazepine is applied in the field of separation and purification of microbial bacteria, which can solve the problems of ineffective removal, frequent detection, and high detection concentration of sewage treatment methods, and achieves a simple, fast, and low-cost separation and purification method. , the effect of easy-to-obtain

Inactive Publication Date: 2010-04-07
DONGHUA UNIV
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Problems solved by technology

Among them, the antiepileptic drug carbamazepine (Carbamazepine, CBZ) (molecular structure such as figure 1 Shown) due to its remarkable drug effect, it is often used as the first choice drug for the treatment of epilepsy and neuralgia. However, carbamazepine is a nitrogen-containing aromatic heterocyclic compound. At present, traditi

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  • Method for extracting efficient degrading bacteria of carbamazepine
  • Method for extracting efficient degrading bacteria of carbamazepine
  • Method for extracting efficient degrading bacteria of carbamazepine

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Embodiment 1

[0057] (1) The activated sludge taken from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt medium by 5% of the access amount, wherein the inorganic salt medium is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0, acclimation by gradient pressure acclimation method, carbamazepine concentration is 3, 5, 10, 15, 20, 22 mg / L, at 30°C, shaking rate 160r / min, Under the condition of 100mL filling volume in 250mL Erlenmeyer flask, the acclimatization culture is carried out, and the total acclimatization time is 2 months;

[0058] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 -1 ~10- 10 For gradient dilution, take 1mL bacterial solution for each dilution concentration into a petri dish, pour the solid medium into it, put the petri d...

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Abstract

The invention relates to a method for extracting efficient degrading bacteria of carbamazepine, which comprises the following steps: (1) taking active sludge of a secondary sedimentation tank of an urban sewage treatment plant, inoculating the active sludge into an inorganic salt culture medium according to an inoculating volume of 5 weight percent, and acclimating the mixture by a gradient pressure type acclimation method for 2 months; and (2) standing a sample after the 2 months of the acclimation for 30 minutes, taking 1 milliliter of an upper layer water sample to perform gradient dilution, taking 1 milliliter of a bacterial solution of each diluted concentration respectively, inoculating the bacterial solution into a solid culture medium to perform enrichment culture so as to ensure that a pure single bacterial strain grows out, then inoculating the single bacterial strain into a slant culture medium, and preserving the mixture at a temperature of 4 DEG C. The method extracts the degrading bacteria of the carbamazepine from the active sludge of the secondary sedimentation tank of the urban sewage treatment plant, the strain source is easy to obtain and has a simple, convenient and quick separation and purification method and a low cost, and is environment-friendly; and the extracted strain has a degradation rate of over 75 percent to the carbamazepine, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of separation and purification of microbial bacteria, in particular to a method for extracting carbamazepine-degrading bacteria with high efficiency. Background technique [0002] With the improvement of environmental analysis technology and the enhancement of people's environmental awareness, pharmaceutical and personal care products (PPCPs) as a new pollutant have been paid more and more attention by people. Drugs and personal care products still have a considerable degree of biological activity even if they exist in trace amounts, causing unpredictable impacts on the environment, and may cause potential harm to human health through drinking water and food. [0003] Among common PPCPs compounds, antibiotics, anti-inflammatory drugs, and antiepileptic drugs were frequently detected in various environmental media. Among them, the antiepileptic drug carbamazepine (Carbamazepine, CBZ) (molecular structure such as figu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/40
Inventor 薛罡杨林刘亚男龚清杰高品史锦程起跃周宁娟王志楼
Owner DONGHUA UNIV
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