Method for extracting efficient degrading bacteria of carbamazepine

Abstract

The invention relates to a method for extracting efficient degrading bacteria of carbamazepine, which comprises the following steps: (1) taking active sludge of a secondary sedimentation tank of an urban sewage treatment plant, inoculating the active sludge into an inorganic salt culture medium according to an inoculating volume of 5 weight percent, and acclimating the mixture by a gradient pressure type acclimation method for 2 months; and (2) standing a sample after the 2 months of the acclimation for 30 minutes, taking 1 milliliter of an upper layer water sample to perform gradient dilution, taking 1 milliliter of a bacterial solution of each diluted concentration respectively, inoculating the bacterial solution into a solid culture medium to perform enrichment culture so as to ensure that a pure single bacterial strain grows out, then inoculating the single bacterial strain into a slant culture medium, and preserving the mixture at a temperature of 4 DEG C. The method extracts the degrading bacteria of the carbamazepine from the active sludge of the secondary sedimentation tank of the urban sewage treatment plant, the strain source is easy to obtain and has a simple, convenient and quick separation and purification method and a low cost, and is environment-friendly; and the extracted strain has a degradation rate of over 75 percent to the carbamazepine, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of separation and purification of microbial bacteria, in particular to a method for extracting carbamazepine-degrading bacteria with high efficiency. Background technique [0002] With the improvement of environmental analysis technology and the enhancement of people's environmental awareness, pharmaceutical and personal care products (PPCPs) as a new pollutant have been paid more and more attention by people. Drugs and personal care products still have a considerable degree of biological activity even if they exist in trace amounts, causing unpredictable impacts on the environment, and may cause potential harm to human health through drinking water and food. [0003] Among common PPCPs compounds, antibiotics, anti-inflammatory drugs, and antiepileptic drugs were frequently detected in various environmental media. Among them, the antiepileptic drug carbamazepine (Carbamazepine, CBZ) (molecular structure such as figu...

AI Technical Summary

Problems solved by technology

Among them, the antiepileptic drug carbamazepine (Carbamazepine, CBZ) (molecular structure such as figure 1 Shown) due to its remarkable drug effect, it is often used as the first choice drug for the treatment of epilepsy and neuralgia. However, carbamazepine is a nitrogen-containing aromatic heterocyclic compound. At present, traditional sewage treatment methods (such as adsorption, activated sludge, Biofilm, ultraviolet, photocatalytic degradation, etc.) cannot be effectively removed, so it is frequently detected, and the detection concentration is relatively high, and it has certain ecological toxicity. It is currently one of the most concerned PPCPs pollutants

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