Method for constructing composite structure tissue engineering bracket containing different extracellular matrixes

A technology of tissue engineering scaffold and composite structure, which is applied in medical science, prosthesis, etc., and can solve problems such as difficult to realize tissue engineering bone construction

Inactive Publication Date: 2010-04-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the cells can only survive through blood diffusion within 200 μm, if the inherent blood circulation cannot be established, the production of tissue engineered tissue can only be limited to a thin and small level, and it is difficult to realize the construction of large tissue engineered bone

Method used

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  • Method for constructing composite structure tissue engineering bracket containing different extracellular matrixes
  • Method for constructing composite structure tissue engineering bracket containing different extracellular matrixes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] a. Polylactic acid was dissolved in chloroform at a concentration of 8% (g / ml), and a stent was prepared by electrospinning technology. The electrospinning voltage is 20KV, the electrospinning distance is 12cm, the flow rate is 0.1ml / h, and the diameter of the receiving metal rod is 1mm.

[0033] b. Take off the vascular stent from the metal rod and dry it in vacuum. A stainless steel metal rod with a diameter of 0.8 mm is used to penetrate into the vascular stent.

[0034] c. Use a standard sieve to sieve 27 g of sodium chloride with a particle size of 224 to 300 μm as a porogen.

[0035] d. Weigh 3 g of polylactic acid and polycaprolactone at a mass ratio of 7:3 and dissolve in 40 ml of chloroform, and stir magnetically to dissolve.

[0036] e. Add the porogen obtained in step c to the polymer solution obtained in step d, stir with a glass rod to disperse the porogen evenly in the polymer solution, and form a pasty mixture containing the porogen with certain fluidit...

Embodiment 2

[0043] a. Polylactic acid-caprolactone copolymer and gelatin were dissolved together in chloroform at a concentration of 8% and 2% (g / ml) respectively, and stents were prepared by electrospinning technology. The electrospinning voltage is 20KV, the electrospinning distance is 12cm, the flow rate is 0.1ml / h, and the diameter of the receiving metal rod is 0.8mm.

[0044] b. Take off the vascular stent from the metal rod and dry it in vacuum. A stainless steel metal rod with a diameter of 0.6mm is used to penetrate into the vascular stent.

[0045] c. Use a standard sieve to sieve 27 g of sodium chloride with a particle size of 224 to 300 μm as a porogen.

[0046]d. Weigh 3 g of polylactic acid and polycaprolactone at a mass ratio of 6:4 and dissolve in 40 ml of chloroform, and stir magnetically to dissolve.

[0047] e. Add the porogen obtained in step c to the polymer solution obtained in step d, stir with a glass rod to disperse the porogen evenly in the polymer solution, and...

Embodiment 3

[0053] a. Polylactic acid and collagen were dissolved together in hexafluoroisopropanol at a concentration of 8% and 0.5% (g / ml) respectively, and the collagen vascular stent was prepared by electrospinning technology. The electrospinning voltage is 20KV, the electrospinning distance is 12cm, the flow rate is 0.1ml / h, and the diameter of the receiving metal rod is 1.5mm.

[0054] b. Take off the vascular stent from the metal rod and dry it in vacuum. A stainless steel metal rod with a diameter of 1.3 mm is used to penetrate into the vascular stent.

[0055] c. Use a standard sieve to sieve 27 g of sodium chloride with a particle size of 224 to 300 μm as a porogen.

[0056] d. Weigh 3 g of polylactic acid and polycaprolactone according to the mass ratio of 8:2 and dissolve in 40 ml of chloroform, and stir magnetically to dissolve.

[0057] e. Add the porogen obtained in step c to the polymer solution obtained in step d, stir with a glass rod to disperse the porogen evenly in ...

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Abstract

The invention belongs to the technical fields of macromolecule materials and biomedical engineering, in particular to a method for constructing a composite structure tissue engineering bracket containing different extracellular matrixes. The method comprises the following steps: preparing a blood vessel bracket by an electrostatic spinning method; preparing a poriferous bracket part by adopting a blend of polylactic acid and polycaprolactone through a hot-pressing and salting-out method; and introducing the blood vessel bracket into the poriferous bracket. The composite structure tissue engineering bracket constructed in the invention realizes that different extracellular matrixes are constructed in a same bracket.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and biomedical engineering, and specifically relates to a method for constructing a tissue engineering scaffold with a composite structure containing different extracellular matrices. Background technique [0002] There are a large number of patients suffering from bone defects, bone tumors, osteomyelitis and other diseases waiting for treatment every year around the world. At present, methods such as autologous bone transplantation and allogeneic bone transplantation are mostly used, but these methods have obvious deficiencies. The concept of tissue engineering and tissue engineering bone provides an option for the treatment of bone diseases. The construction of ideal tissue-engineered bone in vitro has always been a research hotspot in the field of tissue engineering, and great progress has been made. At the same time, the repair of bone defects by tissue-engineered bone has also been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/56
Inventor 任天斌宋莹曹春红王安李兰
Owner TONGJI UNIV
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