Cell electrofusion chip device based on micro-chamber array structure

Abstract

The invention provides a cell electrofusion microelectrode array chip device based on a micro-chamber structure, which consists of a micro-chamber array chip, a periphery printed circuit board and a flow passage control module. The micro-chamber array chip consists of a quartz substrate layer, a metal microelectrode array, polymer side walls and a polymer water surrounding fender, wherein two opposite metal microelectrodes and two opposite polymer sidewalls form one micro-chamber which is arranged in an array shape; the micro-chamber array chip is bonded on the periphery printed circuit board to form electrical connection; the flow passage control module covers the micro-chamber array chip. In the invention, a certain electrical field is formed in the micro-chamber by outside electrical signals so as to control high-efficient queue and electrofusion of cells inside the micro-chamber, thus realizing that only one pair of cells inside the micro-chamber are subjected to fusion, and improving throughout and safety of cell fusion.

Description

technical field [0001] The invention relates to a device for electrical fusion of biological cells. Specifically, the present invention relates to a chip for providing cell electrofusion, providing and generating the electric field intensity and electric field gradient required for cell alignment, electroporation, and fusion. The invention relates to precise control of cells and efficient fusion of cells in cell electric fusion, and is applicable to the fields of genetics, animal and plant distant hybrid breeding, developmental biology, drug screening, monoclonal antibody preparation, mammalian cloning and the like. Background technique [0002] Since the 1980s, cell electrofusion technology has been rapidly developed and widely used because of its high efficiency, simple operation, non-toxicity to cells, easy observation, suitable for instrument application and standardized operation. [0003] Cell electrofusion can be divided into two main stages: cell queuing and cell fu...

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Problems solved by technology

[0007] However, the traditional cell electrofusion equipment also has some disadvantages: (1) Due to the large electrode spacing in the fusion tank, a high external driving voltage is required to achieve sufficient strength of cell queuing, fusion and compaction signals, often as high as several Hundreds of thousands of volts, high requirements on the electrical safety of the system, the cost of the system is also greatly increased; (2) The large distance between electrodes is not conducive to the precise control of cells, etc.

[0010] However, there are still some problems with the above-mentioned chips. For example, although the microfluidic chip researched by MIT in the United States has solved the problem of cell pairing, the distance between the two electrodes of the chip is relatively large, and a high external voltage is still required to be able to achieve electrical fusion

However, the number of microelectrodes integrated in the chip proposed by Zhao Zhiqiang is small, and high-throughput fusion cannot be achieved; the electric field strength and electric field gradient generated by the microelectrodes are relatively weak, and it is difficult to achieve precise control of cells; the corrosion resistance of the selected processing materials , the anti-oxidation ability is also poor; at the same time, because the sample inlet and outlet device is not integrated, the operation is also more inconvenient

The method proposed by Japanese researchers is inefficient, and the fusion throughput is far from meeting the requirements of post-fusion cell research

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