Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore

Abstract

The invention provides a fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, belonging to the technical field of biology. The method comprises the steps of: 1. adding the ganoderma lucidum spore into water or 20-100% alcohol-containing solution or edible acid solution with the pH value of 3-7 or edible alkali solution with the pH value of 7-10, and evenly stirring to prepare mixture, wherein the weight ratio of the ganoderma lucidum spore to the solution is 1:5-15:2; and 2. processing the mixture under normal temperature though high-pressure pulse electric field to break, wherein the electric-field intensity is 10-110 kilovolt/cm and the number of the pulse is 1-800. By using the high-pressure pulse electric field, the method has less heat energy in the process of breaking, low raised temperature of a breaking target, and short breaking time. The breaking mechanism is not breaking the cell wall in the high-pressure pulse electric field but breaking the cell wall by forming a plurality of through holes on the surface of the cell wall by polar molecules in the solution under the function of the high-pressure pulse electric field, thereby improving the use ratio of bioactive substances.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] At present, Ganoderma lucidum has been widely used in medicine, food, beauty and other products, and Ganoderma lucidum spore is the essence of Ganoderma lucidum, and it is also one of the popular drugs for anti-cancer and enhancing the immune function of the body in recent years. The spore wall of Ganoderma lucidum has 3 layers of spore wall (polysaccharide wall) composed of chitin and glucan, which is tough, acid and alkali resistant, and extremely difficult to oxidize and decompose, thus limiting people's digestion and absorption of effective substances in the spore. Most active reports indicate that the main active substances of Ganoderma lucidum are concentrated in Ganoderma lucidum spores, and the active ingredients, medicinal value, and nutritional value are several times higher than those of Ganoderma lucidum fruiting bodies. Among them, Ganoderma lucidum, Ganode...

AI Technical Summary

Problems solved by technology

The disadvantage is that the time is short, the wall cannot be broken, and the time is long, the wall is dissolved, and its effective dissolved substances are also dissolved.

Since the activity of lysing enzyme is greatly affected by external factors such as temperature and humidity, it is difficult to grasp the appropriate lysing time

[0006] 3. High-frequency ultrasonic method: using wet method to break the wall, which consumes a lot of energy

[0007] 4. High-power and high-speed airflow milling method: requires a low temperature environment of -35°C, large investment, high noise, and low production volume. The broken Ganoderma lucidum spores need to be stored at low temperature, and the production cost is high

[0008] 5. Microwave negative pressure method: using wet method to break the wall, pumping air to reduce pressure, small production volume, large investment and high production cost

[0009] 6. The carbon dioxide steam explosion method consumes a lot of energy and is not safe for heat-sensitive substances