Bacteria for degrading cellulose and microbial inoculum prepared by same
A technology for cellulose degrading bacteria and Aspergillus, applied in microorganisms, fungi, microorganism-based methods, etc., can solve the problems of severe reaction conditions, expensive equipment, environmental pollution, etc., and achieve simple fermentation conditions, convenient operation and low cost. Effect
Inactive Publication Date: 2010-06-09
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
Using traditional physical and chemical methods, such as acid treatment, alkali treatment, and steam heating, to treat straw and other cellulose, there are problems such as severe reaction conditions, expensive equipment, high cost, and new environmental pollution.
Method used
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Embodiment Construction
[0016] Examples of the present invention are described below.
[0017] The invention provides a cellulose-degrading bacterium, the strain YN1 of which is isolated from a soil sample collected from compost and identified as Aspergillus sp. The main morphological characteristics are: YN1 grows rapidly on Chase agar medium, the colony is flat and has radial grooves, and the texture is velvety; Spherical to radial; conidiophores occur in the stroma, near the apical capsule with light yellowish brown, smooth walls; sporulation structure single layer, conidia spherical, walls significantly rough, with spines.
[0018] (1) Determination of liquid and solid fermentation enzyme activity of cellulose degrading bacteria YN1
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The invention provides bacteria for degrading cellulose and microbial inoculum prepared by same, which both belong to the bio-technology field. YN1 microbial strain is added to a PDA plating culture medium, PDA liquid culture medium, a seed tank culture medium and a production culture medium, and the effectively live bacteria of the microbial strain reaches over 100 million / ml. After the YN1 bacterial strain is cultivated through fermentation, the activity of incision enzyme, excision enzyme, beta-glycosidase, xylanase and cellulase is respectively 95.7 U / mL, 14.6 U / mL, 20.5 U / mL, 71.6 U / mL and 26.6 U / mL. After the YN1 bacterial strain is cultivated through solid fermentation, the activity of incision enzyme, excision enzyme, beta-glycosidase, xylanase and cellulase is respectively 1192.2 U / g, 100.6 U / g, 136.9 U / g, 1118.0 U / g and 210.7 U / g. The YN1 microbial strain can degrade 41.87% straws and 31. 59% rice husks in 7 days.
Description
1. Technical field [0001] The invention relates to a cellulose-degrading bacterium and a bacterial agent produced therefrom, which belong to the field of biotechnology, degrade cellulose by means of microorganisms, and have good application prospects for decomposing farmland straw and promoting decay of rice husks in bamboo forests. 2. Background technology [0002] Cellulose is the world's most abundant renewable resource, yet it has been underutilized. my country is a large agricultural country with abundant straw resources of various crops. However, straws take a long time to decompose and transform in the soil after being returned directly to the field, making it difficult to use them as a fertilizer source for seasonal crops. At the same time, about 30% of the remaining crop stalks cannot be effectively treated and utilized each year, and the phenomenon of concentrated burning of stalks during the harvest season has become increasingly prominent, polluting the air and e...
Claims
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IPC IPC(8): C12N1/14C05F11/08C12R1/66
Inventor 李顺鹏赵方圆黄星范宁杰朱建春
Owner NANJING AGRICULTURAL UNIVERSITY
