Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial

A time-resolved, superparamagnetic technology, applied in the direction of material inspection products, material magnetic variables, biological testing, etc., can solve the problem that the detection sensitivity needs to be improved, the cost of the magnetic resonance instrument and the singleness of the application method, and the technology cannot really be put into practical application etc. to achieve the effect of reducing interference and improving detection sensitivity

Active Publication Date: 2010-06-09
BEIJING SHUOWANG TECH
View PDF7 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because superparamagnetic nanometers do not exhibit magnetism without magnetization, although using the above-mentioned part of the technology, superparamagnetic nanomaterials can be induced by the sensor to produce a certain degree of magnetism during the test process, but the detection sensitivity still needs to be improved (Biosensors and Bioelectronics, 2005, 20, 1611)
However, some of the above techniques are not suitable for the detection of trace amounts of superparamagnetic na...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial
  • Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial
  • Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A single magneto-impedance sensor is used as a high-sensitivity magnetic-sensitive element to carry out test experiments. The specific test conditions are as follows: the pulse magnetic field strength is 2.5mT; the pulse magnetization time is 2s; The method provided by patent 200710187275.0); the samples to be tested were 12.6 μg, 21.5 μg, 59.6 μg, 168 μg, and 2010 μg; Figure 7 shown. Different amounts of Fe 3 o 4 The data of the detected residual magnetic field strength of the nanocrystal at 200 ms is shown in the table below.

[0085] 11nm Fe 3 o 4 Nanocrystal Sample Volume

Embodiment 2

[0087] The process of protein detection using immunosuperparamagnetic nanoparticles containing single (mononuclear) ferric oxide nanocrystals is as follows: use a capillary glass tube to dissolve 5 μL of the solution to be tested containing p53 protein (protein content: 0.17 μg) in nitric acid Spot the sample on the specific site of the cellulose membrane; then move the nitrocellulose membrane to the blocking solution containing BSA protein for blocking for 1 h; then take out the membrane, make the side with the sample to be tested face down, and place it in Contains Fe with a particle size of 11nm 3 o 4 On the liquid surface of the nanoparticle-p53 protein antibody conjugate solution, incubate at room temperature for 1 to 2 hours, wash twice with TBST buffer for 10 minutes each time, and wash once with TBS for 10 minutes; finally, dry the The final nitrocellulose membrane was placed on the sample holder of the detection unit for residual magnetism detection, and the detectio...

Embodiment 3

[0089] The detection process of Escherichia coli using composite immunomagnetic beads containing a plurality of superparamagnetic nanocrystals is as follows: add Escherichia coli O157:H7 single-coli Immunomagnetic beads for cloning antibodies 100 μL 200nm), incubate for 20min, at this time, the antibody on the surface of the magnetic beads and Escherichia coli undergo an immune recognition reaction; use a magnetic separator to enrich the immune magnetic beads in the sample solution, discard the supernatant, and wash 3 times with PBS buffer; the obtained 10 μL of the immunomagnetic bead enrichment solution was dropped on the substrate modified with Escherichia coli O157:H7 monoclonal antibody on the surface, and incubated for 30 min; the surface of the substrate was washed with PBS buffer to remove unbound immunomagnetic beads; the dried substrate The chip is placed on the sample holder of the detection unit for residual magnetism detection. The detection parameters are: pulse...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a weak magnetism detecting instrument based on time-resolved remanence relaxation detection and application thereof. The detecting instrument consists of a detecting unit provided with a sensitive element with high sensitivity therein, a sample bracket, a pulse magnetic field generator, a signal data acquiring and converting system and a computer. The working principle of the detecting instrument is that the superparamagnetic nanomaterial is magnetized via pulse by means of a pulse magnetic field with a steep edge, then a magnetism sensitive element with a high sensitivity is utilized to carry out track analysis on the remanence relaxation generated by the superparamagnetic nanomaterial under the action of the pulse magnetic field, and then a quantitative detection of superparamagnetic nanomaterial is realized. By means of the instrument, biological analysis with high sensitivity can be realized by combining with nano-particles or magnetic beads. The instrument and method of the invention have fast detection speed, low cost, high sensitivity and good stability, and are suitable for fields of biological analysis, disease diagnosis, food security, environment monitoring and the like.

Description

technical field [0001] The invention belongs to the field of biological and pharmaceutical analysis instruments and analysis methods, in particular to a time-resolved remanence relaxation detector for quantitative detection of superparamagnetic nanometer materials and its application. Background technique [0002] The magnetic properties of superparamagnetic nanomaterials are characterized by the fact that the size of the core nanoparticles is smaller than the size of a single magnetic domain, which is at the nanometer level, so the spin electrons are in a state of random orientation under the action of thermal kinetic energy, and do not exhibit macroscopic magnetic. Under the action of an external magnetic field, superparamagnetic nanomaterials can be magnetized. However, when the external magnetic field disappears, superparamagnetic materials can quickly demagnetize without external demagnetization. This basic characteristic is the reason why superparamagnetic nanomateria...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/72G01N33/48
Inventor 赵唯宇艾希成罗志勇
Owner BEIJING SHUOWANG TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap