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Method for evaluating efficacy of antler

A deer antler and drug efficacy technology is applied in the field of evaluating the influence of deer antler on the growth and metabolism of characteristic microbial flora in the gastrointestinal tract, which can solve the problems of difficulty in comprehensively characterizing characteristic information, inability to obtain dynamic data, and easy loss of overall information, so as to be beneficial to accurate determination. , conducive to standardization, easy to standardize the effect

Inactive Publication Date: 2010-07-07
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Traditional research methods such as cellular immune reaction detection, immunohistochemistry, hemodynamics, and erythrocyte agglutination have been used to evaluate the active parts of velvet antler, but these methods often have single evaluation indicators, easy loss of overall information, and interference factors. It is difficult to fully characterize the characteristic information of the body in the process of drug intervention, and it is impossible to obtain real-time dynamic data.

Method used

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  • Method for evaluating efficacy of antler
  • Method for evaluating efficacy of antler
  • Method for evaluating efficacy of antler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 The effect of each extraction part of velvet antler alcohol extract on the growth and metabolism of beneficial bacteria in the gastrointestinal tract

[0053] 1. Preparation of test solution

[0054] Take 12g of sika velvet antler powder, add 10 times the amount of 80% ethanol to reflux and extract 3 times, 1 hour each time, combine the medicinal solution, and concentrate it into an extract with a relative density of 0.9g / ml; adopt system solvent extraction method with ether and chloroform , Ethyl acetate and n-butanol fractionated extraction of the extract, each solvent was extracted 3 times, 20ml each time, and the extracts were combined to obtain the ether part, chloroform part, ethyl acetate part, and n-butanol part. Each extraction site was dried under reduced pressure to obtain a dry paste; respectively, 0.2g of each extraction site dry paste was taken, and an appropriate amount of sterile water was added to dissolve it to a concentration of 100μg·mL. -1 , 20...

Embodiment 2

[0072] Example 2 Effect of n-butanol extraction parts of velvet antler alcohol extract on the growth and metabolism of harmful bacteria in the gastrointestinal tract

[0073] 1. Measurement method

[0074] Under a constant temperature of 37℃ and sterile conditions, the 1×10 6 Two Staphylococcus aureus were inoculated into the peptone medium to form a suspension of Staphylococcus aureus with a concentration of 1.5×10 6 Cells / mL; Take 0.05ml of the suspension of Staphylococcus aureus, and mix 1.95ml of the test solution with different concentrations of the velvet antler n-butanol extraction site prepared in Example 1 in an ampoule, seal, and use an ampoule lifter Put the ampoule in the preheating position of the upper half of the measuring cylinder, preheat the sample for 20 minutes, then drop the sample into the measuring cup for testing, and record the thermal spectrum curve; when the curve returns to the baseline, the experiment ends. The thermogram curve of growth and metabolism ...

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Abstract

The invention relates to a method for evaluating efficacy of an antler, which takes the bifidobacterium in a gastrointestinal tract as representative of beneficial bacterium and takes staphylococcus as representative of harmful bacterium.The method objectively and quantitatively evaluates the influence of different extraction sites of antler alcohol extract on the growth and metabolism of microorganism in the gastrointestinal tract through obtaining a thermogram and other comprehensive information which can fully reflect the interaction process and has overall expression characteristics, screens the active site with the strongest bacterium-promoting action of the antler and inspects the bacteriostasis of the active side simultaneously. The method has simple operation, easy standardization and automation of the detection.

Description

Technical field [0001] The invention relates to a method for evaluating the efficacy of velvet antler, in particular to a method for evaluating the effect of velvet antler on the growth and metabolism of the characteristic flora of the gastrointestinal tract. Background technique [0002] The antler is the young horns of the stag of Cervus nippon Temminck or Cervuselaphus Linnaeus, which is cervus nippon Temminck or Cervuselaphus Linnaeus. With the deepening of research, more and more chemical components have been extracted and separated from different active parts of velvet antler. There are also many research reports on the pharmacological effects of velvet antler. However, the actual pharmacological basis of velvet antler regulation and tonic is still unclear. Affect its promotion and application. Therefore, objectively and sensitively characterizing the efficacy of velvet antler is of great significance for the quality standard control of velvet antler and clinical effective...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N25/20
Inventor 鄢丹肖小河韩玉梅张萍唐慧英
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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