Preparation method of compound microbial feed additive
A technology of compound microecology and feed additives, applied in animal feed, animal feed, application, etc., to achieve the effect of high-tech level, high activity and advanced technology
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Embodiment 1
[0038] A preparation method of a compound microecological feed additive, comprising the following steps:
[0039] (1) Preparation of organic functional yeast rich in trace elements:
[0040] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 20 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 3% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 18h, add the sodium selenite solution of 3mg / L, continue to cultivate to 45h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;
[0041] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...
Embodiment 2
[0060] A preparation method of a compound microecological feed additive, comprising the following steps:
[0061] (1) Preparation of organic functional yeast rich in trace elements:
[0062] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture at 28°C for 25 hours as seed liquid; prepare pH4. 5 YEPD liquid culture medium, inoculate the brewer's yeast after activation with 6% bacterium weight, 30 ℃ shaking table reciprocating vibration culture after 25h, add the sodium selenite solution of 6mg / L, continue to cultivate to 55h; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;
[0063] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, shake and culture ...
Embodiment 3
[0082] A preparation method of a compound microecological feed additive, comprising the following steps:
[0083] (1) Preparation of organic functional yeast rich in trace elements:
[0084] A. Preparation of selenium yeast: Passage beer yeast twice on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, and shake at 28°C for 23 hours as seed liquid; prepare pH4. 5 YEPD liquid medium, inoculate the activated brewer's yeast with 5% bacterial weight, and after reciprocating shaking culture on a shaker at 30°C for 20 hours, add 5 mg / L sodium selenite solution and continue to cultivate until 48 hours; The fermented liquid was centrifuged at 4000r / min for 10min, the bacteria were collected and dried to obtain selenium yeast;
[0085] B. Preparation of iron yeast: Passage beer yeast on the YEPD slant to obtain activated strains, inoculate the activated strains into the prepared YEPD liquid medium, and shake at 28°C for 22...
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