Method for preparing monostalotetrahexosyl gangliside GM1 compound

A technology for ganglioside and monosialic acid, which is applied in the field of preparing monosialotetrahexosylganglioside GM1 preparations, can solve the problems of difficult application, environmental pollution, complicated separation and purification process, etc., and achieves safe elimination. hidden dangers, improved safety, and improved clinical repair and therapeutic effects

Inactive Publication Date: 2010-08-11
吕维学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

From the description of the patent application for this invention, it can be seen that there are the following deficiencies: first, the separation and purification process is complicated, and many harmful organic solvents are used for enzymatic hydrolysis and addition, which is not only difficult to apply in industry but also causes environmental pollution; The second is that the solvent recovery process uses a membrane with a pore size of 10,000 to 100,000 Daltons, preferably about 50,000 Daltons, for diafiltration. This diafiltration process is a pressure-free natural diafiltration, which is time-consuming and labor-intensive. , and less efficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for preparing monosialotetrahexosyl ganglioside GM1 preparation according to Example 1 of the present invention comprises the following steps:

[0018] (1) Prepare mixed homogenate:

[0019] Collect fresh pig brains from the meat joint factory, remove the broken bones, meninges, skull base blood vessels, etc., wash with distilled water, add 3 times the volume of a mixed solvent of chloroform, methanol, and water after weighing, and the blending ratio is 1:1: 0.5, placed in a high-shear homogeneous emulsification tank, homogenized the raw materials at 6000-8000 rpm for 30 minutes, poured into an 800-mesh stainless steel sieve, filtered, and removed the residue to obtain a lipid mixed homogenate.

[0020] (2) Preparation of GM1 primary extract:

[0021] Send the filtered mixed homogenate into the hanging basket air filter press, and filter through the air compression to remove the residue; take back the filtrate and let it stand for stratification, and collect...

Embodiment 2

[0027] The method for preparing monosialotetrahexosyl ganglioside GM1 preparation according to Example 2 of the present invention comprises the following steps:

[0028] (1) Prepare mixed homogenate:

[0029] Collect fresh pig brains from the meat joint factory, remove the broken bones, meninges, and blood vessels at the base of the skull, wash them with distilled water, add 5 times the volume of a mixed solvent of chloroform, methanol, and water after weighing, and the blending ratio is 1:1: 0.8, put it in a colloid mill and grind it first, then pour it into a high-shear homogeneous emulsification tank and homogenize it at a speed of 8000 rpm for 60 minutes, then pour it into an 800-mesh stainless steel sieve to filter, remove the residue, Obtain lipid mixed homogenate.

[0030] (2) Preparation of GM1 primary extract:

[0031] Send the lipid mixed homogenate into the hanging basket air filter press, and filter through air compression under the condition of vacuum pressure o...

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PUM

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Abstract

The invention discloses a method for preparing monostalotetrahexosyl gangliside GM1 compound, which belongs to the technical field of the biochemical pharmacy. Combining the separation technologies of hyperfiltration membrane and nanofiltration membrane and on the basis of maintaining the bioactivity and improving the curative effect of gangliside GM1 compound, the gangliside GM1 is extracted and purified from pig brain from the process steps such as homogenization, filter pressing, hyperfiltration, nanofiltration and the like, and the purity of the ganliside GMI reaches more than 95 percent. Relevant steps of the traditional chromatograph and column chromatography purification method are reduced, and the application of harmful organic solvent is reduced. The method has the advantages that the industrialized production of the entire process can be realized, the production cost and the production expense can be reduced, not only bioactivity can be maintained, and the curative effect of gangliside GM1 compound can be improved, but also the preparation process is simple and environment-friendly and is free from the pollution.

Description

technical field [0001] The invention relates to the technical field of biochemical pharmacy, in particular to a method for preparing monosialotetrahexosyl ganglioside GM1 preparation. Background technique [0002] Gangliosides are a class of glycolipids with one or more sialic acid residues, which are widely present on the surface of mammalian brain cell membranes and central nervous tissues. It is known that gangliosides can repair nervous system damage , promote the growth and regeneration of nerves, promote the recovery of innervation function, and protect nerve cell membranes. [0003] Monosialotetrahexosyl ganglioside is one of the main gangliosides, which can be used in many medical applications, especially in the repair and treatment of central and peripheral nervous system disorders, and can promote central Nerve remodeling and functional recovery after nervous system injury have a good curative effect on cerebral and spinal cord trauma, cerebral ischemic injury and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61P25/00A61P25/16A61P9/10
Inventor 吕维学
Owner 吕维学
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