Compound microbial agent and production method and application thereof
A technology of compound microbial inoculum and Enterococcus faecalis, applied in application, animal feed, animal feed, etc., can solve the problems of single function of microbial feed additive, single metabolite composition, difficult product quality control, etc., and achieve great practicality and promotion. value, improve economic efficiency, and improve the effect of disease resistance
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[0010] Example 1
[0011] The preparation method of the compound microbial inoculum: the multiplication and cultivation of Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis are calculated in parts by weight of Candida utilis: Enterococcus faecalis: Lactobacillus acidophilus : Bacillus subtilis == 1:1:1:1, mix the above-mentioned strains and ferment them in a culture medium at a constant temperature of 10% by mass percentage, and stir while oxygen is supplied. The initial temperature of the culture is 28°C for 24 hours. Cultivate for 24 hours at 30°C and 12 hours at 35°C until the fermentation culture reaches pH 2.5.
[0012] The culture medium is 1g beef extract 1g agar, 2.5g yeast extract, 35g sucrose, 1.5g defoamer (commercially available product, purchased from Meichen Group Co., Ltd. in this example), 1g peptone, and distilled water to 1000ml pH3.0~5.0, 110~130℃ autoclave sterilization. The yeast extract is yeast extract powder (commercial...
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[0025] Example 2 Preparation method of composite microbial inoculum: the multiplication and cultivation of Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus, and Bacillus subtilis are calculated as parts by weight of Candida utilis: Enterococcus faecalis: Lactobacillus acidi: Bacillus subtilis==1:2:2:1, the above-mentioned strains are mixed and fermented in a culture medium at a constant temperature of 15% by mass, and agitated while aerating oxygen. The initial temperature of the culture is 28℃. For 24 hours, culture at 30°C for 24 hours, 35°C for 12 hours, until the fermentation culture reaches pH 3. The medium is 0.5 g of agar, 0.5 g of beef extract, 2 g of yeast extract, 30 g of sucrose, 3.0 g of antifoaming agent, 0.5 g of peptone, and 1000 mL of distilled water. pH3.0~5.0, 110~130℃ autoclave sterilization. The yeast extract is yeast extract powder.
[0026] The above-mentioned composite microbial inoculum was used as an additive for feeding ducks. The feedi...
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[0031] Example 3
[0032] The preparation method of the compound microbial inoculum: the multiplication and cultivation of Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis are calculated in parts by weight of Candida utilis: Enterococcus faecalis: Lactobacillus acidophilus : Bacillus subtilis==3:1:1:1, mix the above-mentioned strains and ferment them in a culture medium at a constant temperature of 12% by mass percentage, and stir while aerating oxygen. The initial temperature of the culture is 28°C for 24 hours. Cultivate for 24 hours at 30°C and 12 hours at 35°C until the fermentation culture reaches pH 3.5. The medium is 1.5 g of agar, 1.5 g of beef extract, 2 g of yeast extract, 45 g of sucrose, 2 g of antifoaming agent, 1.5 g of peptone, and 1000 mL of distilled water. pH3.0~5.0, 110~130℃ autoclave sterilization. The yeast extract is yeast extract powder.
[0033] The above-mentioned composite microbial inoculum was used as an additive ...
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