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Compound microbial agent and production method and application thereof

A technology of compound microbial inoculum and Enterococcus faecalis, applied in application, animal feed, animal feed, etc., can solve the problems of single function of microbial feed additive, single metabolite composition, difficult product quality control, etc., and achieve great practicality and promotion. value, improve economic efficiency, and improve the effect of disease resistance

Inactive Publication Date: 2010-08-18
沈阳科丰牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The compound microbial flora mostly uses bacteria of the same species and genus with similar or similar characteristics to form compound probiotics. This compound bacteria has a single component of bacterial metabolites; the effect is not ideal; the product effect is unstable; Control and other issues
The microbial feed additives produced by single flora and compound flora have the disadvantages of single efficacy, roughness, lack of system, and poor acidification of feed, which brings a lot of inconvenience to the development of microbial feed additives

Method used

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  • Compound microbial agent and production method and application thereof
  • Compound microbial agent and production method and application thereof
  • Compound microbial agent and production method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0011] The preparation method of the composite microbial bacterial agent: count the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis in parts by weight after the propagation and cultivation: Enterococcus faecalis: Lactobacillus acidophilus : Bacillus subtilis==1: 1: 1: 1, after mixing the above-mentioned strains, 10% by mass percentage is fermented at a constant temperature in the culture medium, and stirred while passing oxygen, the initial temperature of the culture is 28 ℃ for 24 hours, Cultivate at 30°C for 24 hours and at 35°C for 12 hours until the fermentation culture reaches a pH value of 2.5.

[0012] The medium is 1g of agar, 1g of beef extract, 2.5g of yeast extract, 35g of sucrose, 1.5g of defoamer (commercially available product, purchased from Meichen Group Co., Ltd. in this embodiment), 1g of peptone, and distilled water is added to 1000ml pH3.0~5.0, autoclave at 110~130℃. The yeast extract is yeast extract powder (commerci...

Embodiment 2

[0025] The preparation method of embodiment 2 composite microbial bacterial agents: the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis after the proliferation culture are calculated in parts by weight. Candida utilis: Enterococcus faecalis: Enterococcus faecalis Lactobacillus acidum: Bacillus subtilis == 1:2:2:1, mix the above strains and ferment in the culture medium at a constant temperature of 15% by mass, and stir while passing oxygen. The initial temperature of the culture is 28°C. 24 hours, culture at 30°C for 24 hours, and culture at 35°C for 12 hours, until the fermentation culture reaches a pH value of 3. The culture medium is 0.5 g of agar, 0.5 g of beef extract, 2 g of yeast extract, 30 g of sucrose, 3.0 g of defoamer, 0.5 g of peptone, and 1000 mL of distilled water. pH3.0~5.0, autoclave at 110~130℃. The yeast extract is yeast extract powder.

[0026] With above-mentioned compound microbial inoculum as the additive of raisi...

Embodiment 3

[0032] The preparation method of the composite microbial bacterial agent: count the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis in parts by weight after the propagation and cultivation: Enterococcus faecalis: Lactobacillus acidophilus : Bacillus subtilis==3: 1: 1: 1, the above-mentioned strains are mixed and fermented at a constant temperature in the culture medium by 12% by mass percentage, and stirred while passing oxygen, and the initial temperature of the culture is 28° C. for 24 hours. Cultivate at 30°C for 24 hours and at 35°C for 12 hours until the fermentation culture reaches a pH value of 3.5. The culture medium is 1.5g of agar, 1.5g of beef extract, 2g of yeast extract, 45g of sucrose, 2g of defoamer, 1.5g of peptone, and 1000mL of distilled water. pH3.0~5.0, autoclave at 110~130℃. The yeast extract is yeast extract powder.

[0033] The above-mentioned compound microbial inoculum was used as an additive for raising cattle,...

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Abstract

The invention relates to a feed additive, in particular to a compound microbial agent and a production method thereof. Candida utilis, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis are compounded to be cultured in a culture medium until the pH value is 2.5-3.5 to obtain the compound microbial agent in parts by weight: 1-3 parts of Candida utilis, 1-3 parts of enterococcus faecalis, 1-3 parts of lactobacillus acidophilus and 1-3 parts of bacillus subtilis. The compound microbial agent can serve as the feed additive of animals. The invention adopts the mode that various strains are combined to obtain strong acid state flora with different acidity degrees according to culture medium components, culture temperature and different ratios of various strains; according to animal types and different adopted feeds, and the advantages of the strong acid state flora can be performed to improve animal disease resistance, improve economic benefit and optimize environment.

Description

technical field [0001] The invention relates to a feed additive, in particular to a compound microbial agent and its production method and application. Background technique [0002] At present, most of the existing microbial additive products are mainly cultivated by a single strain. The compound microbial flora mostly uses bacteria of the same species and genus with similar or similar characteristics to form compound probiotics. This compound bacteria has a single component of bacterial metabolites; the effect is not ideal; the product effect is unstable; control issues. The microbial feed additives produced by such single flora and compound flora have the disadvantages of single efficacy, roughness, lack of systematicness, and poor acidification of feed, which brings a lot of inconvenience to the development of microbial feed additives. Contents of the invention [0003] One of the objectives of the present invention is to provide an aerobic, anaerobic and facultative ...

Claims

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Application Information

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IPC IPC(8): A23K1/17A23K20/195
Inventor 高林白子金朱立良白春生海洋何凤燕邵中金潘永荣刑成俭聂鑫孙艳娇徐海涛王跃男杨忠伟侯树东
Owner 沈阳科丰牧业科技有限公司
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