Method for separating and extracting natural theanine

A theanine, natural technology, applied in the separation/purification of carboxylic acid amide, organic chemistry, etc., can solve the problems of limited life, high cost, cumbersome resin regeneration operation, etc., achieve less usage, good mechanical properties, and easy regeneration Effect

Inactive Publication Date: 2010-08-18
晋江市恒源科技开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the cumbersome regeneration operation of resin, l...

Method used

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  • Method for separating and extracting natural theanine

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Effect test

Embodiment 1

[0025] Commercially available FAU zeolite powder (silicon-alumina ratio: 72) was processed into strips, activated in air at 550°C for four hours and cooled, placed in deionized water, degassed by ultrasonic, and filled into an adsorption column. Adsorption column height: 45cm, inner diameter: 3cm, zeolite adsorbent loading capacity 118g.

[0026] Weigh 1 g of theanine, prepare a 0.002 g / mL theanine solution, and adjust the pH value to 1-5 with hydrochloric acid-NaOH. At room temperature (25°C), pump 500mL of theanine aqueous solution to the adsorption column with a constant flow pump, adjust the flow rate to stabilize at 6.0mL / min, and analyze the theanine content of the effluent by sampling every 50ml. Elute with 0.2mol / L NaCl solution, collect the eluate, and analyze the content of theanine in the eluate. After 2-3 column volumes, the elution is basically complete, and the recovery rate is 80%.

Embodiment 2

[0028] Commercially available FAU zeolite powder with a silicon-to-aluminum ratio of 15 was processed into pellets with a particle size of 1.5 mm, calcined at 600°C for 3.5 hours, and treated with water vapor at 120°C for 2 hours. Placed in deionized water, degassed by ultrasonic, loaded into adsorption column. Adsorption column height: 45cm, inner diameter: 3cm, zeolite adsorbent loading capacity: 123g.

[0029] Weigh 5 grams of tea powder, prepare 0.01 g / mL tea, and filter to remove insoluble impurities. Adjust the flow rate to be stable at 8.0mL / min, pump 500mL of tea water solution to the adsorption column with a constant flow pump, and keep the temperature of the adsorption column at 60°C. After the tea solution is pumped out, immediately pump in deionized water until the effluent is colorless. Theanine was eluted with 1mol / L ammonia water, and the eluate was collected. Vacuum rotary evaporation to remove ammonia, concentrate to about 1 / 5 of the initial volume of the so...

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Abstract

The invention belongs to the technical field of deep processing of tea, particularly relates to a method for separating and extracting natural theanine. At the temperature of 5-75 DEG C, aqueous solution containing theanine is enabled to flow through an adsorbing column filled with inorganic zeolite molecular sieve adsorbent; the theanine is adsorbed; ammonia spirit is used to elute the theanine adsorbed by the adsorbing column; alcohol is added after vacuum evaporation and concentration are carried out on the eluent; the theanine is separated out when the temperature is cooled to be about 0 DEG C; pumping filter is carried out; and recrystallization is carried out for 1-3 times to obtain a product the theanine content of which is more than 90%. The adsorbent can be repeatedly regenerated and reused, and the operational process is safe, simple and convenient and has low energy consumption. The invention is suitable for separating and extracting the theanine from a tea leaching solution, is especially suitable for processing low-grade tea, rough and old tea, tea tree roots and the like, or can be used for utilizing leftovers, waste liquor containing the theanine and other plants containing the theanine in the deep processing process of the tea.

Description

technical field [0001] The invention belongs to the technical field of tea deep processing, and in particular relates to a method for extracting theanine from tea extract, tea processing leftovers and waste liquid containing theanine. Background technique [0002] The chemical name of theanine is N-ethyl-γ-amino-L-glutamine, and its structural formula is as follows: [0003] [0004] Theanine is a unique amino acid in tea, which can be decomposed into L-glutamic acid and ethylamine, and ethylamine can be oxidized to form ethyl acetate, which makes the tea soup have the refreshing taste of MSG and the sweetness of caramel. The content of theanine has a certain positive correlation with the quality of tea. [0005] Modern medical and pharmacological studies have shown that theanine can antagonize the nervous system excitement caused by caffeine, relieve emotional tension, lower blood pressure, protect nerve cells, enhance the efficacy of anticancer drugs, inhibit cancer ce...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24
Inventor 林德昌龙英才李润泽林金建
Owner 晋江市恒源科技开发有限公司
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