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Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace

A technology for bacterial cellulose and fruit juice, applied in the field of producing edible colored bacterial cellulose, can solve the problems of difficult environmental protection, environmental pollution, unpleasant smell, etc., and achieve the effects of low cost, high color fastness and high added value.

Active Publication Date: 2010-08-18
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And every year, a large number of fruits with poor quality or discarded due to certain elements need to be disposed of, and these raw materials are good raw materials for the production of bacterial cellulose. If they are not used, they will become domestic waste. Environmental pollution has become a major problem in environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of fruit juice culture solution: wash the silt and impurities on the peel of discarded oranges with running water, beat the pulp with a beater and peel off the peel, and wrap the fruit pulp with cloth to extract the juice; the juice after coarse filtration is filtered through a centrifugal filter , Separation, remove the remaining peel, seeds, part of the fiber, crushed pulp and impurities. The pH of the juice was adjusted to 4.5 using sodium dihydrogen phosphate buffer, the juice was sterilized, and then stored at -5°C for future use.

[0024] (2) Preparation of bacterial cellulose: select the above-mentioned orange juice culture solution prepared after dilution of 2 times as a culture medium; inoculate Acetobacter xylinum with 0.1wt% inoculum, carry out constant temperature cultivation at 30°C, and carry out bacterial cellulose production within 7 days Production.

[0025] (3) The obtained bacterial cellulose was rinsed and deacidified, then boiled in...

Embodiment 2

[0027] (1) Preparation of fruit juice culture medium: pour the discarded apple fruit after washing into the squeezer to squeeze the juice, and then filter the peel, fruit seeds and some crude fibers through a scraper filter; the juice after the rough filtration is centrifuged Machine filtration and separation to remove residual peel, fruit seeds, some fibers, crushed pulp and impurities to obtain juice without solid particles; use sodium dihydrogen phosphate buffer to adjust the pH of the juice to 3.5, and sterilize the juice. Then store at -20°C for later use;

[0028] (2) Preparation of bacterial cellulose: select the above-mentioned prepared apple juice culture solution diluted 5 times, add 2wt% yeast extract and 0.1wt% sodium citrate to the culture solution, and sterilize again as a culture medium ; Inoculate lactic acid bacteria with 0.5wt% inoculation amount, carry out constant temperature cultivation at 32° C., and carry out bacterial cellulose production within 14 days...

Embodiment 3

[0031] (1) Preparation of pomace culture solution: Saccharify the waste orange peel and the residues in the fruit juice preparation process to obtain a saccharification solution, use sodium dihydrogen phosphate buffer to adjust the pH of the saccharification treatment solution to 3.7, and carry out the saccharification treatment solution. Sterilize, then store at -10°C for later use;

[0032] (2) Preparation of bacterial cellulose: select the above-mentioned prepared orange pomace culture solution after being diluted 8 times, add 5wt% concentration to be 30wt% sugar water, 0.6wt% ammonium sulfate in this culture solution, and sterilize again as Culture medium: Acetobacter xylinum is inoculated with an inoculum amount of 0.8 wt%, and cultured at a constant temperature of 30° C., and the production of bacterial cellulose is carried out within 12 days.

[0033] (3) The obtained bacterial cellulose was rinsed and deacidified, then boiled in 10wt% sugar water, and soaked in sugar w...

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Abstract

The invention relates to a method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace. The method comprises the following steps: (1) fruit juice culture solution is prepared, (2) pomace culture solution is prepared, (3) the bacterial cellulose is prepared, (4) the obtained bacterial cellulose is disacidified in drift ice, boiled in 10 to 30 wt percent of sweet water and then is dipped in the sweet water for 12 to 24h when the bacterial cellulose is still hot and the edible colorful bacterial cellulose can be obtained. Compared with the overseas synthesis culture base method, the utility model has the advantages of low cost and high yield. The invention can develop various edible colorful bacterial cellulose products with different colors, high color fastness, good taste and rich nutrition value.

Description

technical field [0001] The invention relates to a method for producing edible colored bacterial cellulose by using agricultural by-products, in particular to a method for producing edible colored bacterial cellulose by using waste fruit juice or pomace as raw materials. Background technique [0002] As an emerging nano-biological material, bacterial cellulose has been widely concerned by all walks of life. Bacterial cellulose (also known as microbial cellulose) is a kind of nanofiber prepared by biological method. It is a three-dimensional network structure composed of nanofibers with a width of about 10nm and a thickness of about 3-8nm. Its chemical composition and molecular structure It is the same as natural plant cellulose, but without lignin, pectin and hemicellulose associated with plant cellulose. It has a high crystallinity of up to 95%, a degree of polymerization as high as 2000-8000, and a strong water-holding capacity. , has high biocompatibility, adaptability an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/645C12R1/225
Inventor 胡伟立陈仕艳张泽锐王华平黄锦荣
Owner DONGHUA UNIV
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