Analysis method for fast detecting degradation rate of organophosphorus degrading bacteria

An analysis method, organophosphorus technology, is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve problems such as long duration, need for pretreatment, and complicated operation process. Achieve the effect of simple instrument, low cost and easy operation

Inactive Publication Date: 2010-08-18
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

This method is accurate in detection, but requires pretreatment

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  • Analysis method for fast detecting degradation rate of organophosphorus degrading bacteria

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Embodiment 1

[0014] 1) The organophosphate-degrading strain Bacillus megaterium was inserted into the dichlorvos-inorganic salt medium with an inoculum volume of 10%, wherein the concentration of dichlorvos was 200 mg / L (the total phosphorus concentration was 28.0 mg / L). Cultivate at 30°C and 180r / min for 48h.

[0015] 2) The supernatant obtained by centrifuging the fermentation broth at 8° C. at 6000 r / min for 30 min is the sample solution to be tested in the present invention.

[0016] 3) Draw 5mL of the sample solution to be tested, add 0.1-0.2mL dinitrophenol indicator, then dilute it with water in a 50mL volumetric flask, then add 5% by volume sulfuric acid solution to adjust the solution until it is slightly yellow. Accurately add 5mL molybdenum antimony anti-color agent, shake well, add water to make up volume. At room temperature, let stand for 30min to develop color. Put the color-developed sample solution on the spectrophotometer, use a 700nm, 1cm optical path cuvette, and use ...

Embodiment 2

[0020] 1) The organophosphate-degrading strain Microbacterium oxidans was inserted into the dichlorvos-inorganic salt medium with a volume ratio of 5% inoculum, wherein the concentration of phoxim was 400 mg / L (total phosphorus concentration was 41.6 mg / L). Cultivate at 30°C and 180r / min for 24h.

[0021] 2) The supernatant obtained by centrifuging the fermentation broth at 8° C. at 6000 r / min for 20 minutes is the sample solution to be tested in the present invention.

[0022] 3) Draw 2.5mL of the sample solution to be tested, add 0.05-0.1mL dinitrophenol indicator, then dilute it with water in a 50mL volumetric flask, and then add 10% volume percent sodium carbonate solution to adjust the solution until it is slightly yellow . Accurately add 5mL molybdenum antimony anti-color agent, shake well, add water to make up volume. At room temperature, stand for 20min to develop color. Put the color-developed sample solution on the spectrophotometer, use a 700nm, 1cm optical path ...

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Abstract

The invention relates to the technical filed of biological degradation of pesticide residue, in particular to an analysis method for fast detecting the degradation rate of organophosphorus degrading bacteria. The method comprises the following steps: inoculating organophosphorus degrading bacterial strains into an organophosphorus-inorganic salt culture medium according to the inoculating amount with the volume ratio of 5 percent to 10 percent for fermentation culture; carrying out centrifugation after the fermentation to obtain supernate which is a sample solution to be detected; adding 0.2 to 0.4 mL indicating agents of dinitrophenol into every 10 mL sample solution to be detected; then, adopting water for defining the volume to 50 mL; then, adding 10 percent sodium carbonate solution or 5 percent sulfuric acid solution into the solution; next, adding 5 mL molybdenum-stibium color developing resistant agents to be uniformly shaken after the solution become light yellow; adding water for defining the volume; placing the solution for 20 to 30 min for color development under the condition of room temperature; carrying out colorimetric determination at 700 nm after the color development; and checking and obtaining the corresponding phosphor content from a calibration curve. The ratio of the obtained phosphor content to the total phosphor concentration in organophosphorus farm insecticide is the degradation rate of the organophosphorus degrading bacteria. The analysis method of the invention can be used for direct analysis of biochemical samples without any pretreatment.

Description

technical field [0001] The invention relates to the technical field of biodegradation of pesticide residues, in particular to an analysis method for rapidly detecting the degradation rate of organic phosphorus-degrading bacteria. Background technique [0002] Organophosphorus is a broad-spectrum insecticide, which has been widely used in my country due to its advantages of high efficiency, broad spectrum and low price. Because organic phosphorus residues cause environmental and groundwater pollution, and also cause serious harm to food safety, solving organic phosphorus residues, especially biodegradable organic phosphorus, has become one of the hotspots in the field of pesticide residue degradation. [0003] Screening efficient degrading strains is the most critical step in microbial degradation of organophosphorus pesticides. At present, the common method to detect the degradation rate of degrading strains is to use chromatography. This method is accurate in detection, b...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 张惠文蔡颖慧苏振成李旭李新宇
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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