Novel technique for extracting L-tryptophan from fermentation broth

A technology for tryptophan and fermentation broth, applied in the new technological field of separating and extracting L-tryptophan, can solve the problems of easy product deterioration, environmental pollution, low extraction yield, etc., achieve thorough sterilization and impurity removal, and improve extraction Yield, effect of short filtration time

Active Publication Date: 2010-08-25
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems of easy deterioration of the product, low extraction yield and serious environmental pollution in the industrialization proce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] The first step is to pre-treat the fermentation broth containing L-tryptophan produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO3 of the fermentation broth volume, stir and heat to 60°C for 30 min. Treatment liquid

[0025] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the pressure difference between the inlet and outlet membranes is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 The purified water is top-washed until the tryptophan content in the concentrate is less than 1.0g / L, and then the machine is shut down to obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria are removed. The yield was 99.3%;

[0026] The third step, the tryptophan microfiltrate is pumped into t...

Example Embodiment

[0032] Example 2:

[0033] The first step is to pre-treat the fermentation broth containing L-tryptophan produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO3 of the fermentation broth volume, stir and heat to 60°C for 30 min. Treatment liquid

[0034] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the difference between the inlet pressure and the outlet pressure is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 The purified water is top-washed until the tryptophan content in the concentrate is less than 1.0g / L, and then the machine is shut down to obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria are removed. The sterilization rate reaches 99.74%, and L-tryptophan The yield was 99.3%;

[0035] Th...

Example Embodiment

[0041] Example 3:

[0042] The first step is to pre-treat the fermentation broth containing L-tryptophan produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO3 of the fermentation broth volume, stir and heat to 60°C for 30 min. Treatment liquid

[0043] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the difference between the inlet pressure and the outlet pressure is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 Top wash with purified water until the tryptophan content in the concentrate is less than 1.0g / L, then stop. Obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria have been removed; the bacteria removal rate reached 99.74%, and the yield of L-tryptophan was 99.3%;

[0044] The third step, the ...

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PUM

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Abstract

The invention discloses a novel technique for extracting L-tryptophan from fermentation broth, which includes the following steps that: fermentation broth is pretreated, and the treated liquid is pumped into a ceramic microfiltration membrane filter, so that tryptophan microfiltrate without mycelium and mycoprotein are obtained; the tryptophan microfiltrate is pumped into an ultrafiltration membrane filter, so that tryptophan ultrafiltrate and concentrated liquid containing pigment and protein are obtained; the tryptophan ultrafiltrate is pumped into a nanofiltration membrane filter, so that concentrated tryptophan liquid and permeate containing salt are obtained; waste filtrate is comprehensively utilized to produce protein feed; industrial ethylic acid is used for promoting low-temperature crystallization, crystallization mother liquid is treated by the ion exchange method and used, and after crystals are centrifugally dehydrated and dried, the L-tryptophan product is obtained. The invention solves the problem on how to recover industrial ethylic acid, and greatly increases the extraction rate of L-tryptophan; and since the filtrate and the mycoprotein are comprehensively utilized, the product profit is maximized. The total extraction rate of L-tryptophan is 85.2 percent, and the refined product meets the standards of Chinese Pharmacopoeia edition 2005.

Description

Technical field: [0001] The invention relates to the field of biochemical engineering, in particular to a new process for separating and extracting L-tryptophan from fermentation broth. Background technique: [0002] According to the calculation of the Food and Agriculture Organization of the United Nations in 2000, the annual demand for L-tryptophan in the world market is about 1.2 million tons or more. In order to promote the application of L-tryptophan, L-tryptophan, whose price has always been relatively high, hopes to have a cheap and efficient production and extraction method. Therefore, the market gap of L-tryptophan is very large and the prospect is bright. The scientific name of L-tryptophan is β-indolylalanine, the chemical name is L-2-amino-3-indolylpropionic acid, alias L-trypsin amino acid, L-aminoindolepropionic acid, molecular formula C 11 h 12 o 2 N 2 , relative molecular mass 204.21. L-Tryptophan is one of the eight essential amino acids in human and a...

Claims

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Application Information

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IPC IPC(8): C07D209/20A23K1/00
Inventor 赵春光邓立君左善孟宗盘
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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