Method for quickly precipitating and separating oversulfated chondroitin sulfate in sodium heparin
A technology of chondroitin polysulfate and precipitation separation, applied in the biological field, can solve the problems such as no reports of separation technology, insignificant separation effect, poor separation effect, etc., and achieve the effect of remarkable separation effect, improved color and lustre, and remarkable effect.
Active Publication Date: 2010-09-08
江苏麦德森制药有限公司
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Problems solved by technology
[0004] Separation of chondroitin polysulfate from heparin sodium, so far, except for the organic solvent extraction separation method, there is no other report on separation technology
And the organic solvent extraction separation method has the following defects: the one, because chondroitin polysulfate and heparin sodium belong to polysaccharides, the solubility to organic solvents such as ethanol, acetone is very low, so the separation effect of the organic solvent extraction method is not good, especially The separation effect is less obvious when the polysulfate chondroitin in heparin sodium is higher
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[0027] (1) Dissolving: open the water valve, add 1500L of drinking water in the reactor, while stirring, 15 billion total titers of crude product heparin sodium (crude product heparin sodium titer is 100IU / mg, the weight ratio of crude product heparin sodium and water is 1:10) into the reaction kettle, turn on the steam, raise the temperature to 55°C±5°C, and stir for 5 hours to completely dissolve the heparin sodium;
[0028] (2) Filtration: filter with a 100-mesh filter screen, wash the filter residue with a small amount of water, collect the filtrate and lotion (the two are combined);
[0029] (3) Resin adsorption: pump the filtrate into the treated 3000L anion exchange resin adsorption column, control the flow rate at 50L / h, and circulate the effluent into the barrel for 24 hours;
[0030] (4) Cleaning: stop the adsorption after the titer of the effluent is detected to be constant. Absorption residual liquid is emptied. Clean the resin with water, and then prepare 10000L...
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The present invention discloses a method for quickly precipitating and separating oversulfated chondroitin sulfate in sodium heparin. The method adopts an ion exchange method to remove a mass of impurities combined with a hydrogen peroxide oxidation method, which greatly improves the color and the potency of the sodium heparin, quickly precipitates and separates the oversulfated chondroitin sulfate in the sodium heparin by alcohol, which ensures the yield coefficient of the sodium heparin more than 85 percent and the potency more than 180u/mg, and each index totally satisfies the USP and EP standards. The present invention is more remarkable than the reported acetone extraction method; because the solvability of the oversulfated chondroitin sulfate and sodium heparin is very low to ethanol and acetone and the like, the separating effect of the organic solvent extraction method is bad, specifically, the separating effect is more unconspicuous when the oversulfated chondroitin sulfate in sodium heparin is higher. The quick precipitation method is designed according to the feature that the absolute molecular weight of the oversulfated chondroitin is greater than the sodium heparin and the differences of structure and the like. The method is featured with simple operation and notable separating effect.
Description
Technical field: [0001] The invention belongs to the field of biotechnology and relates to a method for separating chondroitin polysulfate from heparin sodium. Background technique: [0002] Heparin sodium is derived from the small intestinal mucosa of pigs. It is an acidic mucopolysaccharide containing sulfate groups. It is a typical anticoagulant drug, which can prevent the coagulation process of blood and prevent the formation of thrombus. Because heparin sodium can inhibit the conversion of prothrombin into thrombin with the participation of α-globulin. Heparin sodium also has the functions of clarifying plasma lipids, lowering cholesterol and enhancing the effectiveness of anticancer drugs. It is widely used clinically to prevent thrombosis and embolism before and after surgery, to prevent blood coagulation during blood transfusion and as an anticoagulant for preserving fresh blood. Eliminate uremia caused by nephropathy in children. Heparin ointment is also widely u...
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IPC IPC(8): C08B37/10C08B37/08
Inventor 刘榜惠张志斌袁红英
Owner 江苏麦德森制药有限公司
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