Application of golden thread detoxication decoction as compound preparation in preparing IOD active medicaments

A technology of Huanglian Jiedu Decoction and compound preparation, applied in the field of traditional Chinese medicine, can solve problems such as lack of new IDO inhibitor drugs

Inactive Publication Date: 2010-09-22
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of shortage of high-efficiency and novel IDO inhibitor drugs, and to provide a new medicinal application of the compound preparation Chinese medicine Huanglian Jiedu Decoction, specifically related to the use of the compound preparation Huanglian Jiedu Decoction in the preparation of active drugs for inhibiting IDO

Method used

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  • Application of golden thread detoxication decoction as compound preparation in preparing IOD active medicaments
  • Application of golden thread detoxication decoction as compound preparation in preparing IOD active medicaments
  • Application of golden thread detoxication decoction as compound preparation in preparing IOD active medicaments

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of recombinant IDO was carried out as described in the literature.

[0020] The IDO activity detection was carried out on a 96-well plate. First, 50 mM potassium phosphate buffer (pH 6.5), 40 mM vitamin C, 400 μg / ml catalase, 20 μM methylene blue and IDO enzyme were mixed. Then add the substrate L-tryptophan and the sample to be tested into the above mixed solution. The reaction was carried out at 37°C for 60 minutes, and the reaction was terminated by adding 30% (w / v) trichloroacetic acid. The 96-well plate was heated at 65°C for 15 minutes to complete the conversion from formylkynurenine to kynurenine, and then spun at 6000 g for 5 minutes. Take out 100 μl of supernatant from each well and transfer it to a new 96-well plate, add 2% (w / v) p-(dimethylamino)benzaldehyde in acetic acid solution, the yellow color produced by the reaction of kynurenine It can be observed at 490nm using a microplate reader.

Embodiment 2

[0021] Example 2. IDO inhibitory activity of Huanglian Jiedu Decoction

[0022] Coptis chinensis, Scutellaria baicalensis, Cortex Phellodendron and Gardenia were decocted twice according to the original ratio (3:2:2:3), adding 10 times and 8 times of water to decoct for 60 and 40 minutes respectively, combined the decoction liquid, concentrated to each One milliliter is equivalent to 1 gram of crude drug. The IDO inhibitory activity test of Huanglian Jiedu Decoction and IDO inhibitor 1-methyltryptophan (1-MT) was compared, and the results showed that the Huanglian Jiedu Decoction had obvious inhibitory efficiency on IDO.

[0023] Table 1. Inhibition efficiency of Huanglian Jiedu Decoction on IDO

[0024]

Embodiment 3

[0025] Example 3. IDO inhibitory activity of each single herb in Huanglian Jiedu Decoction

[0026] Each crude drug is soaked in ten times the volume of methanol for seven days, the soaking solution is concentrated into a paste, and dissolved in an appropriate amount of methanol, so that each milliliter of the solution is equivalent to 1 gram of crude drug. The IDO inhibitory activity of each crude drug was tested and compared with the IDO inhibitor 1-MT, and the results showed that each single drug in the Coptidis Jiedu Decoction showed inhibition of IDO activity, and Coptis chinensis had a significant inhibitory effect on IDO activity.

[0027] .

[0028] Table 2. Inhibition efficiency of each single drug in Huanglian Jiedu Decoction on IDO

[0029]

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Abstract

The invention belongs to the field of traditional Chinese medicines and relates to new medicament application of a golden thread detoxication decoction as a compound preparation, in particular to application of the golden thread detoxication decoction as the compound preparation in preparing IOD active medicaments. In the invention, an in-vitro IOD activity detecting system is established by using recombined human IDO enzyme, and experimental results indicate that the golden thread detoxication prepared from Chinese medicinal crops of golden thread, baical skullcap roots, golden cypress and Fructus Gardeniae as well single medicine and single chemical components of the composition thereof have IDO inhibition activities. The golden thread detoxication decoction and the single medicine of the composition thereof can be used for inhibiting IDO and can be used as a medicament raw material for preparing medicaments for treating or preventing diseases with the pathological characteristics of IDO-mediated tryptophane metabolic pathways.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medicinal application of a compound preparation Huanglian Jiedu Decoction, in particular to the application of the compound preparation Huanglian Jiedu Decoction in preparing a drug for inhibiting IDO activity. Background technique [0002] IDO (indoleamine-2,3-dioxygenase; MW 48,000; EC 1.13.11.42) is an intracellular heme-containing enzyme, which is the only one outside the liver that can catalyze tryptophan to make it along the canine urine The rate-limiting enzyme of the amino acid pathway catabolism. IDO exists in many tissues and cells of humans and animals. It is expressed at a low level under normal conditions, and its expression increases significantly during inflammation or infection. Cytokines IFN-γ and lipopolysaccharide LPS can induce its expression. [0003] Studies have shown that IDO causes the absence of tryptophan in the cellular microenvironment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/756A61P25/22A61P25/24A61P27/02A61P35/00A61P37/02A61K31/352A61K31/4375
Inventor 杨青匡春香黄伟达
Owner FUDAN UNIV
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