Rhodotorula mucilaginose strain and application thereof in degradation of butachlor

A technology of yeast and glue red, applied in the field of bioremediation, can solve problems such as high toxicity, groundwater and river pollution, and impact on human health, and achieve rich and diverse effects

Inactive Publication Date: 2010-09-29
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the one hand, butachlor leaches and diffuses vertically and horizontally with water, especially with the loss of irrigation water and precipitation, which will cause groundwater and river pollution; on the other hand, butachlor is harmful to many microorganisms such as algae, photosynthetic bacteria, and nitrogen-fixing bacteria The growth of butachlor has inhibitory effect, thereby destroying the

Method used

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  • Rhodotorula mucilaginose strain and application thereof in degradation of butachlor
  • Rhodotorula mucilaginose strain and application thereof in degradation of butachlor
  • Rhodotorula mucilaginose strain and application thereof in degradation of butachlor

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example 1

[0014] Example 1. Isolation, purification and identification of Rhodotorula mucilaginose Y-LCY-1 CGMCCNo.3270

[0015] Rhodotorula mucilaginose (Rhodotorula mucilaginose) Y-LCY-1 CGMCCNo.3270 provided by the present invention is isolated and screened from paddy field soil where butachlor has been applied for a long time in the suburbs of Harbin, and its specific isolation and purification process is as follows:

[0016] Take 10g of soil sample and place it in 50mL of butachlor with a concentration of 100mg·L -1 In the basic inorganic salt culture medium, cultivate on a constant temperature shaker at 28°C and 160rpm, then insert 10% of the inoculum into the fresh butachlor inorganic salt culture medium every other week, and gradually increase the dosage of butachlor until the culture The concentration of butachlor in the liquid reaches 1000mg·L -1 , so acclimated for about three months. Then separate on the butachlor-based inorganic salt agar plate by dilution coating method,...

example 2

[0035]Preparation of Example 2 Glue Rhodotorula mucilaginose (Rhodotorula mucilaginose) Y-LCY-1 CGMCCNo.3270 microbial agent

[0036] The specific production process of the bacterial agent is as follows:

[0037] Inoculate the strains preserved in the slant culture medium with 100 mg·L -1 Activated in the inorganic salt medium of butachlor for 12h, then the bacterial solution (OD 600 =1.0) transfer in the conical flask that the YEPD liquid medium of 150mL is housed by the inoculum size of 10% (V / V), the initial concentration of butachlor in the medium is 50mg L -1 , sealed with a parafilm, placed in a constant temperature shaking incubator at 28°C and cultured at a frequency of 160rpm for 3 days, then took out 1 / 4 of the culture solution, and then added the same amount of culture solution to make the initial concentration of butachlor in the medium reach 50mg·L -1 , and continue to cultivate in the incubator for 3 days, which is the bacterial agent. The obtained liquid bac...

example 3

[0039] Example 3 Biodegradation of butachlor by Rhodotorula mucilaginose Y-LCY-1 CGMCCNo.3270

[0040] (1) Rhodotorula mucilaginose Y-LCY-1 CGMCCNo.3270 to 100mg L -1 Determination of Butachlor Degradability

[0041] Bacterial suspension preparation: pick a ring of Y-LCY-1 slant-preserved strains under sterile conditions, transfer them to McFarland’s medium, place them in a 28°C, 160rpm constant temperature shaker, and cultivate them to the optimum bacterial age. A certain amount of culture solution was centrifuged at 4000rpm for 10min, the supernatant was discarded, and 0.2mol·L -1 Wash with phosphate buffer (pH=7) to remove the nutrients carried by the bacteria (3 times in total), collect the bacteria, adjust the concentration of the bacteria with buffer, and measure the absorbance value OD at 600 nm with a spectrophotometer 600nm , so that OD 600nm Around 2.0.

[0042] According to the inoculation amount of 5%, the above bacterial suspension was inoculated in the basic ...

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Abstract

The invention discloses a butachlor degradation strain and application thereof. The butachlor degradation strain is rhodotorula mucilaginose Y-LCY-1 and is preserved in China General Microbiological Culture Collection Center on September 14, 2009 with a preservation number of GMCCNo.3270. The strain can maximally tolerate 1,000 mg.L<-1> butachlor; and after being cultured in an inorganic salt basal medium containing 100 mg.L<-1> butachlor for 7 days at 25 DEG C with the inoculum concentration of 5 percent and pH of 6.5, the strain can degrade 97.6 percent of the butachlor and has good degradation effect on the butachlor of 50 to 500 mg.L<-1>. The strain provided by the invention can be applied to the biological purification of water bodies and soil polluted by the butachlor.

Description

technical field [0001] The invention belongs to the technical field of bioremediation, and relates to a strain of Rhodotorula colloides and its application in degrading the herbicide butachlor. Background technique [0002] Butachlor (C 17 h 26 ClNO 2 ) is a high-efficiency systemic conduction type amide pre-emergence herbicide, mainly used to control barnyard grass in paddy fields and gramineous weeds in dry fields. It has the characteristics of low dosage, high activity, and strong selectivity. It is widely used in my country For production and application, the environmental pollution caused by its residue in water and soil will also become more and more serious. On the one hand, butachlor leaches and diffuses vertically and horizontally with water, especially with the loss of irrigation water and precipitation, which will cause groundwater and river pollution; on the other hand, butachlor is harmful to many microorganisms such as algae, photosynthetic bacteria, and nitr...

Claims

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Application Information

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IPC IPC(8): C12N1/16A62D3/02C02F3/34B09C1/10C12R1/645A62D101/04C02F101/38
Inventor 李春艳成小松徐春红张美超臧海莲熊明华孙晶
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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