Yeast strain and method for preparing phage bdellovibro preparation by using the same

Abstract

The invention discloses a novel strain XD03S (CCTCC NO: M 2010036) of yeast, which is capable of being the parasitichost of phage bdellovibro. The invention further discloses a method for preparing phage bdellovibro preparation by using the yeast strain, which comprises the following steps of: (1) phage bdellovibro purification: taking escherichia coli as target cell, adopting liquid cultivation and a double-layer tap water agar combination method to separate phage bdellovibro base strains from the nature, then adopting a single dental plague passage method to carry out liquid cultivation and the double-layer tap water agar combination method to carry out continuous single dental plague passage purification of at least 10 generations, and obtaining the phage bdellovibro inbred strains with strong phagocytosis capability; and (2) culturing the phage bdellovibro inbred strains obtained in the step (1) and the yeast strain XD03S (CCTCC NO: M 2010036) in a composite way, and obtaining the phage bdellovibro preparation. The preparation method simplifies the production technology of the phage bdellovibro, and reduces sewage discharge and energy consumption.

Description

technical field [0001] The invention relates to a yeast strain, and at the same time, the invention also relates to a method for preparing a phage Bdellovibrio preparation by using the yeast strain. Background technique [0002] Bdellovibrio phage is a small parasitic Gram-negative bacterium discovered by American scientists Stolp and Petzlod in 1962. It has a unique life cycle, one is the free-swimming stage outside the host cell, and the other is the growth and reproduction stage inside the host cell. The peculiar way of life of bacteriophage Bdellovibrio has aroused people's widespread interest. In 1963 it was named Bdellovibrio bacteriophage. [0003] Bdellovibrio phage is a parasitic bacterium that specializes in preying on bacteria. It is smaller than ordinary bacteria and acts like a phage, but it is not a virus and has all the morphological characteristics of bacteria. Bdellovibrio phage is one of the self-purification factors in nature. It is not easy to parasiti...

AI Technical Summary

Problems solved by technology

[0007] 1. Most Escherichia coli are harmful bacteria, which will cause pollution if you are not careful, and the risk is relatively high. Although there are some engineering strains such as the C600 strain of Escherichia coli, which are non-pathogenic bacteria, they are not beneficial bacteria and cannot be used as probiotics direct use;

[0008] 2. It is necessary to centrifugally concentrate the cultivated Escherichia coli, take out the bacteria and inactivate them, which will produce a large amount of centrifugal wastewater, and the inactivation process requires a lot of energy consumption;

[0009] 3. The terminal Bdellovibrio product is a single Bdellovibrio product, and its function is very limited