Yeast strain and method for preparing phage bdellovibro preparation by using the same

A technology for phagocytosing leech vibrio and yeast, applied in the field of yeast strains, can solve the problems of requiring a lot of energy consumption, not belonging to beneficial bacteria, limited functional effects, etc., to reduce sewage discharge and energy consumption, enhance functions, The effect of simplifying the production process

Active Publication Date: 2010-09-29
广州市粤莱晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. Most Escherichia coli are harmful bacteria, which will cause pollution if you are not careful, and the risk is relatively high. Although there are some engineering strains such as the C600 strain of Escherichia coli, which are non-pathogenic bacteria, they are not beneficial bacteria and cannot be used as probiotics direct use;
[0008] 2. It is necessary to centrifugally concentrate the cultivated Escherichia coli, take out the bacteria and inactivate them, which will produce a large amount of centrifugal wastewater, and the inactivation process requires a lot of energy consumption;
[0009] 3. The terminal Bdellovibrio product is a single Bdellovibrio product, and its function is very limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of raw materials:

[0035] 1) Collection of water samples

[0036] Collect water samples and bottom mud from the breeding pond, and seal them up to prepare as the source of bacteria for the isolation test of the basic strain of Bdellovibrio bacteriophage.

[0037] 2) Preparation of host bacteria

[0038] Purchase Escherichia coli C600 from the Culture Collection Center of Guangdong Institute of Microbiology as the pre-host bacteria, make C600 bacteria suspension respectively, centrifuge at 3500r / min to form bacteria sludge, and make the bacteria slurry into 1010cfu / ml bacteria suspension, One part is not sterilized, that is, live bacteria slime C600, and the other part is sterilized, that is, inactivated bacteria slime C600. After treatment, put it in a 4-degree refrigerator for storage until use.

[0039] (2) Isolation of Bdellovibrio

[0040] Separation was carried out using a combination of liquid culture and double-layer tap water agar:

[0041] 1)...

Embodiment 2

[0053] The difference from Example 1 is:

[0054] (5) Screening of composite hosts

[0055] Take the yeast strain XD03S CCTCC NO: M 2010036) and 3 strains of lactic acid bacteria, 2 strains of yeast, and 8 strains of Bacillus probiotics in a compound culture experiment to screen the strains that can symbiotic with XD03S, and finally screen out 1 strain that can symbiotic with XD03S Lactic acid bacteria, 1 strain of yeast, and 1 strain of Bacillus, these three strains are subjected to compound culture to obtain a compound host bacterium, the culture temperature of the above compound culture is 36°C, the pH condition is 7.8, and the medium is: 20 grams of molasses, egg white powder 5 grams, 1000 ml of water.

[0056] (6) Cultivation of phage Bdellovibrio preparations

[0057] The culture medium used in this step is: 20 grams of molasses, 5 grams of egg white powder, 1000 milliliters of water, and the pH condition is 7.8. Use 1 strain of yeast XD03S (CCTCC NO: M 2010036) and 1...

Embodiment 3

[0059] The difference from Example 1 is:

[0060] (5) Screening of composite hosts

[0061] The compound culture experiment of strain yeast XD03S (CCTCC NO: M 2010036) and 3 strains of lactic acid bacteria, 2 strains of yeast, and 8 strains of Bacillus probiotics was carried out to screen the strains that can co-exist with XD03S, and finally screen out 1 strain that can co-exist with XD03S. strain of lactic acid bacteria, 1 strain of saccharomyces, and 1 strain of bacillus. These three strains are subjected to compound culture to obtain a compound host bacterium. The culture temperature of the above compound culture is 30°C, the pH condition is 5.5, and the medium is: 15 grams of molasses, egg white Powder 3.5 grams, water 1000 milliliters.

[0062] (6) Cultivation of phage Bdellovibrio preparations

[0063] The culture medium used in this step is: 15 grams of molasses, 3.5 grams of egg white powder, 1000 milliliters of water, and the pH condition is 5.5. Use 1 strain of ye...

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PUM

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Abstract

The invention discloses a novel strain XD03S (CCTCC NO: M 2010036) of yeast, which is capable of being the parasitichost of phage bdellovibro. The invention further discloses a method for preparing phage bdellovibro preparation by using the yeast strain, which comprises the following steps of: (1) phage bdellovibro purification: taking escherichia coli as target cell, adopting liquid cultivation and a double-layer tap water agar combination method to separate phage bdellovibro base strains from the nature, then adopting a single dental plague passage method to carry out liquid cultivation and the double-layer tap water agar combination method to carry out continuous single dental plague passage purification of at least 10 generations, and obtaining the phage bdellovibro inbred strains with strong phagocytosis capability; and (2) culturing the phage bdellovibro inbred strains obtained in the step (1) and the yeast strain XD03S (CCTCC NO: M 2010036) in a composite way, and obtaining the phage bdellovibro preparation. The preparation method simplifies the production technology of the phage bdellovibro, and reduces sewage discharge and energy consumption.

Description

technical field [0001] The invention relates to a yeast strain, and at the same time, the invention also relates to a method for preparing a phage Bdellovibrio preparation by using the yeast strain. Background technique [0002] Bdellovibrio phage is a small parasitic Gram-negative bacterium discovered by American scientists Stolp and Petzlod in 1962. It has a unique life cycle, one is the free-swimming stage outside the host cell, and the other is the growth and reproduction stage inside the host cell. The peculiar way of life of bacteriophage Bdellovibrio has aroused people's widespread interest. In 1963 it was named Bdellovibrio bacteriophage. [0003] Bdellovibrio phage is a parasitic bacterium that specializes in preying on bacteria. It is smaller than ordinary bacteria and acts like a phage, but it is not a virus and has all the morphological characteristics of bacteria. Bdellovibrio phage is one of the self-purification factors in nature. It is not easy to parasiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20A61K35/74A61P31/04C12R1/645C12R1/01
CPCY02A50/30
Inventor 杨雄黄洁容
Owner 广州市粤莱晟生物科技有限公司
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