Method for quickly testing melamine content in milk powder by using gold nanoparticle colorimetric method
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A gold nanoparticle and melamine technology, which is applied in the field of analytical chemistry, can solve the problems of high detection cost, many pretreatment steps, and cannot be widely used, and achieves the effect of sensitive detection
Inactive Publication Date: 2010-09-29
JIANGNAN UNIV
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Although these methods have high sensitivity, there are many pre-treatment steps for samples, which are relat...
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Embodiment 1
[0033] (1) Preparation of gold nanoparticles: all glass instruments used are soaked in aqua regia, washed with double distilled water, and dried for subsequent use; during preparation, add 100 mL of chloroauric acid with a mass concentration of 0.01% in a clean Erlenmeyer flask, heat , boiled, followed by adding 2.4 mL of trisodium citrate solution with a mass concentration of 1%, stirring while heating, the color of the solution changed from light yellow to red, and the reaction continued for 6-8 minutes. Finally, the solution was cooled to room temperature and washed with double Make up to 100mL with distilled water, and store at 4°C; the average particle size of the prepared gold nanoparticles is 20nm.
[0034] (2) Derivatization of crown ethers:
[0035] ①2-Hydroxymethyl-18-crown ether-6-C 6 - Preparation of Br
[0036] Weigh 4.5 mmol, 0.11 g of sodium hydride (65%-55% mineral oil) and dissolve it in 5 mL of dimethyl sulfoxide solution, add 1.94 mmol (0.57 g) of 2-hydrox...
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Abstract
The invention discloses a method for quickly testing melamine content in milk powder by using a gold nanoparticle colorimetric method, which belongs to the technical field of analytical chemistry. The invention comprises the steps of preparing gold nanoparticles (AuNPs), derivatizing crown ether, conjugating gold nanoparticle and crown ether, self assembling by applying the gold nanoparticles, detecting melamine by molecular recognition, detecting the practical sampling and the like. The invention prepares a gold nanoparticle self-assembly material, can simply, quickly and flexibly detect the melamine content in the milk powder according to the color change of a gold nanoparticle system, simultaneously improves the detection sensitivity greatly, achieves the purpose of high flux, and provides convenience for the subsequent research, production, supervision and the like.
Description
technical field [0001] The invention discloses a gold nanoparticle colorimetric method for rapidly detecting melamine content in milk powder, which belongs to the technical field of analytical chemistry. Background technique [0002] Melamine, commonly known as melamine, protein essence, chemical name is 1,3,5-triazine-2,4,6-triamino, is a triazine nitrogen-containing heterocyclic organic compound, pure white single Prismatic crystals. Melamine is a basic organic chemical intermediate product with a wide range of uses, and its main use is as a raw material for the production of melamine-formaldehyde resin (MF). The resin has higher hardness than urea-formaldehyde resin, is non-flammable, water-resistant, heat-resistant, has good insulation performance, gloss and mechanical strength, and is widely used in wood, plastic, paint, paper, textile, leather, electrical, pharmaceutical and other industries. [0003] In the food industry, the Kjeldahl method is usually used to calcu...
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