Complex enzyme preparation and application in producing diosgenin
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A compound enzyme preparation and diosgenin technology, applied in the direction of enzymes, enzymes, hydrolytic enzymes, etc., can solve problems such as pollution, lack of economic feasibility, immature technology, etc., to solve water pollution, reduce wastewater discharge, and reduce production cost effect
Active Publication Date: 2011-12-07
苏州盛泽科技创业园发展有限公司
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Problems solved by technology
However, due to various reasons such as immature technology, failure to fundamentally solve the pollution problem or lack of economic feasibility, it has not been put into industrial production.
Method used
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Embodiment 1
[0016] A compound enzyme preparation, which includes two sets of compound enzyme preparations, the type I compound enzyme preparation includes alkaline pectinase, cellulase, xylanase, hemicellulase, tannase, and each gram of type I compound enzyme In the preparation, the enzyme activity of alkaline pectinase is 3000IU / g, the enzyme activity of cellulase is 180IU / g, the enzyme activity of xylanase is 6000IU / g, and the enzyme activity of hemicellulase is 1500IU / g , the enzyme activity of tannase is 80IU / g; the type II compound enzyme preparation includes glucoamylase and β-glucosidase, and in each gram of type II compound enzyme preparation, the enzyme activity of glucoamylase is 150IU / g, β-glucosidase The enzyme activity of glucosidase is 120IU / g.
[0017] According to the components of turmeric, the present invention selects two sets of compound enzyme preparations, type I compound enzyme preparation, which are used to loosen and diverge the tissue structure of yam, so that th...
Embodiment 2
[0030]In the first hydrolysis step (3), adjust the solid-liquid ratio to 1:6, pH value to 6.0, add 1% of the type I compound enzyme preparation based on the dry weight of yam, and hydrolyze at 45°C for 10 hours.
[0031] In the secondary hydrolysis step (4), adjust the pH to 6.0, add 0.5% of the type II compound enzyme preparation based on the dry weight of yam, and hydrolyze at 65°C for 1.5 h.
[0032] The present invention is to recycle waste sugar liquid, which is a pollutant. In step (5), the filtered liquid is filtered and washed with water, and the filtrate is reduced in pressure and concentrated. Coarse sugar powder can be used as raw materials in fermentation industry, feed industry, and bio-organic fertilizer industry.
[0033] The same as embodiment 1:
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Abstract
The invention discloses a complex enzyme preparation and application thereof in producing diosgenin. The complex enzyme preparation comprises an I-type complex enzyme preparation and an II-type complex enzyme preparation, wherein the I-type complex enzyme preparation comprises pectinase, cellulose, xylanase, hemicellulase and tannase; the II-type complex enzyme preparation comprises glucose and beta-glucuroide. The application of the complex enzyme preparation in producing diosgenin comprises the following steps of pretreating materials; carrying out primary hydrolysis with the I-type complexenzyme preparation; carrying out secondary hydrolysis with the II-type complex enzyme preparation; filtering the material liquid to enable solid-liquid separation; carrying out acidolysis, water-washable acid and filter-press on a solid product, drying to obtain a saponin hydrolysate; and then extracting and crystallizing according to the traditional process of saponin extraction to obtain a saponin finished product. The diosgenin produced by using the complex enzyme preparation can promote the release of active ingredients of turmeric, improve the yield, reduce the total use level of acid bymore than 70% compared with the traditional process, recycle pollutants-waste sugar liquid at the same time, effectively solve the problem of water pollution in producing the diosgenin, decrease the total amount of the pollutants by more than 85% and reduce the waste discharge of diosgenin production enterprises by 40%.
Description
technical field [0001] The invention relates to the extraction of plant saponins, in particular to a compound enzyme preparation and its application in the production of diosgenin. Background technique [0002] Dioscorea scutellaria, also known as turmeric, is a perennial herbaceous vine plant. Dried yams scutellaria scutellaria contains 33%-40% of plant fiber, 45%-55% of starch, 9%-13% of water-soluble substances, and 2% of saponin. 5﹪. Its tubers have high medicinal and chemical value. After processing, they can extract pharmaceutical and chemical products such as saponin and dithin. At present, hundreds of steroid hormones such as hydrocortisone, prednisone, and dexamethasone can be synthesized. drug. It is an essential ingredient of hormone drugs, known as the "mother of hormones", and is widely used in cosmetics, health care, contraceptive analgesia, anesthesia and other drugs. It is a medicinal material with relatively stable market demand. The saponin produced in m...
Claims
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Application Information
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