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Total glycosides of Rhodiola rosea, medical application and preparation method thereof

A technology of rhodiola rosea and rose, applied in the fields of phenylethanol glycosides, flavonoid glycosides, phenylpropenyl alcohol glycosides and total terpene glycosides, which can solve the problems of lack of conclusion

Active Publication Date: 2010-10-20
于非
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on its material basis is still inconclusive

Method used

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  • Total glycosides of Rhodiola rosea, medical application and preparation method thereof
  • Total glycosides of Rhodiola rosea, medical application and preparation method thereof
  • Total glycosides of Rhodiola rosea, medical application and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: the preparation of formula I compound:

[0096] 2.0kg rhizome of Rhodiola rosea was crushed and extracted three times with 95% ethanol (10, 8, 8 times the amount) under reflux for 3 hours each time, the extracts were combined, and the solvent was recovered under reduced pressure. The concentrated extract was diluted with an appropriate amount of water, and extracted with equal volumes of petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence to obtain 20 g, 33 g, 62 g, and 204 g of the extracts of each layer, respectively. See figure 1

[0097] Take 56 g of the extract from the ethyl acetate extraction layer, use normal pressure silica gel column chromatography, and use chloroform-methanol system gradient elution to obtain Fr.1~999, and combine Fr.122~170 to obtain the compound ( 3, 20); Combined Fr.311~326 was purified by repeated use of Sephadex LH-20 column chromatography with methanol as mobile phase to obtain compound (2); Combined F...

Embodiment 2

[0098] Example 2: Extraction and preparation of total glycosides of rhodiola rosea

[0099] Rhodiola rosea rhizome 2.0kg was crushed, reflux extracted three times with 95% ethanol (10, 8, and 8 times the amount), each time for 3 hours, and the extracts were combined. Ethanol was recovered under reduced pressure to obtain a syrupy extract. Suspend and dissolve with an appropriate amount of water, and elute with water, 10% ethanol, 60% ethanol, and 95% ethanol in sequence through macroporous resin PD101 column chromatography to obtain water-eluted fractions, 10% ethanol-eluted fractions, and 60% ethanol-eluted fractions, respectively. Elution fraction, 95% ethanol elution fraction. Among them, the 60% ethanol eluted part is rich in total rhodiola rosea glycosides, and the total rhodiola rosea glycosides content reaches more than 80%. The 10% ethanol elution part and the 95% ethanol elution part also contain rhodiola rosea total glycosides, and its content is slightly lower, bu...

Embodiment 3

[0100] Example 3: HPLC-TOF-MS Analysis of Total Rhodiola Rosides

[0101] Configuration of the reference substance solution: Accurately weigh an appropriate amount of the reference substance listed in Table 1, and prepare an appropriate amount of the reference substance solution with methanol.

[0102] Configuration of sample solution: Accurately weigh an appropriate amount of Rhodiola rosea flavonoids, Rhodiola rosea, Rhodiola rosea extract or total glycosides of Rhodiola rosea sample, and mix with 100ml methanol (or chloroform, ethyl acetate, acetone, acetonitrile , petroleum ether, cyclohexane and other organic solvents and their different concentrations of aqueous solution) extraction, recovery of the extract, methanol (or chloroform, ethyl acetate, acetone, acetonitrile, petroleum ether, cyclohexane and other organic solvents and their different concentrations of aqueous solution) dissolved, pretreated by SPE small chromatographic column filled with ODS, washed with 95% m...

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Abstract

The invention relates to total glycosides of Rhodiola rosea, medical application and a preparation method thereof. In the invention, the total glycoside extract prepared from the Rhodiola rosea contains flavone glycosides, phenethyl alcohol glycosides, cinnamyl alcohol glycosides and total terpenoid glycosides, and is mainly characterized by containing Rhodiola rosea flavone (1).

Description

Technical field: [0001] The present invention relates to a total glycoside extract isolated from Rhodiola Rosea (Rhodiola Rosea L.), which is characterized in that it contains flavonoid glycosides, phenylethanol glycosides, phenylpropenol glycosides and total terpene glycosides . Background technique: [0002] Rhodiola belongs to the genus Rhodiola (Crassulaceae) of the sedum family (Crassulaceae). There are more than 90 species in the world, and there are more than 70 species in China. It is mainly distributed in the Himalayas, Northwest Asia and North America. It is mainly produced in Northeast, North and Southwest China. Rhodiola plants are mostly used as medicine in the folk. They have the effects of promoting blood circulation and stopping bleeding, clearing the lungs and relieving coughs. They are used to treat hemoptysis, hemoptysis, pneumonia cough, women's leucorrhea [15] In ancient times, it was also used as a tonic and strong medicine. Rhodiola rosea was first ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61K31/353A61K36/41A61P9/00A61P9/08A61P39/06A61P37/02A61P7/02A61P1/16A61P25/28A61P9/10A61P35/00A61P3/06A23L1/30A61K125/00A23L33/105
Inventor 李国玉张珂李晓东卢立娜魏秀岩
Owner 于非
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