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Yeast with strong capacities of tolerating, enriching and converting organic selenium and application thereof

An organic selenium and yeast technology, applied in the field of yeast, can solve the problems of high production cost and lack of good bacteria, and achieve the effects of good stability and strong selenium enrichment ability.

Active Publication Date: 2010-10-20
山东宝来利来生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that there are no good strains, resulting in high production costs. Therefore, it is urgent to screen and cultivate a strain with strong selenium-enriching ability

Method used

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  • Yeast with strong capacities of tolerating, enriching and converting organic selenium and application thereof
  • Yeast with strong capacities of tolerating, enriching and converting organic selenium and application thereof
  • Yeast with strong capacities of tolerating, enriching and converting organic selenium and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The screening of embodiment 1 bacterial classification

[0032] At present, the method of biological selenium enrichment is mainly the microbial conversion method. The microorganisms used for selenium enrichment include edible fungi, yeast and probiotics. The commonly used strain introduced in the literature is Saccharomyces cerevisiae, and Candida utilis is a high-biomass yeast adopted according to the suggestion of the company's internal expert committee. At the same time, Saccharomyces cerevisiae and Candida utilis are fungal strains that can be used in health food as stipulated by the Ministry of Health (see the "Regulations on the Declaration and Review of Fungal Health Foods" promulgated in 2005). Therefore, Candida utilis and Saccharomyces cerevisiae were determined as backup strains for screening. Mainly investigate the selenium enrichment, tolerance and biomass transformation ability of the strain.

[0033] 1 strain screening

[0034] 1.1 Test equipment

...

Embodiment 2

[0061] The screening of embodiment 2 culture medium

[0062] 1 Materials and methods

[0063] 1.1 Materials

[0064] Strain: Candida utilis (Candida utilis) CUM, which was deposited in China Center for Type Culture Collection on April 19, 2010, and its preservation number is: CCTCC M 2010090.

[0065] Medium for primary screening: Cha's medium, PDA medium, wort medium, bean sprouts medium.

[0066] Repeat medium:

[0067] (1) The medium screened out initially

[0068] (2) Add the essential elements needed for the growth of yeast to the medium screened out, that is, add 2% glucose, 1% NH 4 h 2 PO 4 , 0.0001% CaCl 2 , 0.3% KH 2 PO 4 , 0.0001% MnSO 4 , 0.1%Na 2 HPO 4 , 0.1% MgSO 4 ·7H 2 O, 0.05% peptone, 0.0001% FeSO 4 , 0.0001% ZnSO 4 , 0.1% foam enemy, adjust the pH to 6.0.

[0069] 1.2 Method

[0070] Fermentation in Erlenmeyer flasks was used as a comparative test, with 50mL / 500mL Erlenmeyer flasks, 8% inoculum, two parallels, 30°C shaker at 200rpm for 48h, ...

Embodiment 3

[0079] Embodiment 3 different selenium addition time effect test

[0080] In order to investigate the effect of adding sodium selenite on strain growth and selenium enrichment at different stages of strain growth, this experiment selected three time points at the initial stage of fermentation (0h), logarithmic growth stage (22h) and mature stage (30h). Sodium selenite was added to the fermentation broth until the selenium concentration in the culture broth was 20 μg / mL, and the culture was terminated after 46 hours. The test results are shown in Table 6.

[0081] Table 6 Effects of different selenium addition methods on strain growth and selenium enrichment

[0082]

[0083] It can be seen from Table 6 that the biomass of the strain obtained by adding selenium in the initial medium was the lowest, only 2.89g / 100mL, which indicated that the 20 μg / mL selenium concentration in the fermentation broth still inhibited the growth of the strain to a certain extent. Comparing the ...

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Abstract

The invention discloses a strain of yeast with strong capacities of tolerating, enriching and converting organic selenium, which is named Candida utilis CUM and has been preserved in China center for type culturecollection on April 19, 2010 with the preservation number of CCTCC M 2010090. The Candida utilis CUM has the bacterial characteristics that: cells are round, oval or sausage-shaped, and performs vegetative propagation in a mode of multi-edge germination to form pseudohypha; wort solid culture is carried out, and the bacterial colony is milk white, has smooth and moist surface, gloss or no gloss, and neat or hypha-shaped edges; the Candida utilis CUM is chemoheterotrophic, and can ferment glucose, cane sugar and raffinose; and the Candida utilis CUM is facultatively anaerobic, performs aerobic respiration under the aerobic conditions and performs alcoholic fermentation under the anaerobic conditions. The Candida utilis has strong selenium enriching capacity and good stability, can be used for producing selenious yeast, and is a strain with high research and development value.

Description

technical field [0001] The invention relates to a yeast strain with strong organic selenium tolerance, enrichment and transformation capabilities, and belongs to the field of biotechnology. Background technique [0002] Selenium is one of the essential micronutrient elements for humans and animals, and is the active ingredient of glutathione peroxidase in animals. This enzyme can remove peroxides and hydroxyl radicals produced in animal cell respiration and metabolism, and maintain biofilms. The integrity of the body, thereby improving the body's immunity, protecting the liver and anti-aging effects. The lack of selenium in the human body will lead to the decline of immunity, and in severe cases, it will lead to the occurrence of "Keshan disease" and "Kashin-Beck disease". Selenium deficiency in animals can lead to a variety of diseases, such as muscular dystrophy, chicken liver necrosis, nutritional encephalomalacia, piglet mulberry heart, foal diarrhea, pancreatic fibrosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/72
Inventor 单宝龙谷巍张志焱杨立华程秀芳刘虹
Owner 山东宝来利来生物工程股份有限公司
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