Citrus fruit fly protein bait, and preparation method and application method thereof
A technology of Bactrocera citrus and protein, which is applied in the direction of botanical equipment and methods, applications, and attractant pest agents, can solve the problems of environmental pollution and waste of resources, and achieve the goal of no environmental pollution, low cost, and obvious lure effect Effect
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[0039] Example 1, 1) preparation of brewer's yeast hydrolyzate:
[0040] a. Inactivation: Pasteurization is used to pasteurize the active brewer's yeast slurry at 65°C and cool it down to room temperature naturally.
[0041] b. Filtration: first filter with 80 mesh screen, and then filter with 100 mesh screen to remove impurities in beer yeast mud,
[0042] c. Concentrate by heating: heat and boil on a magnetic stirrer for 2 hours, then naturally cool to room temperature,
[0043] d. Proteolysis: add distilled water to the concentrated yeast liquid to prepare a yeast liquid substrate with a yeast mass fraction of 40%-50%, add 0.12g-0.24g of papain in every 100ml of yeast liquid substrate, stir and mix, Use NaOH and HCl to adjust the pH value to 6.5, set the temperature to 65°C, keep warm in a water bath, and carry out the enzymatic hydrolysis reaction for 12 hours.
[0044] e. Terminate the enzymatic hydrolysis reaction: after the enzymatic hydrolysis reaction is completed, ...
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