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Citrus fruit fly protein bait, and preparation method and application method thereof

A technology of Bactrocera citrus and protein, which is applied in the direction of botanical equipment and methods, applications, and attractant pest agents, can solve the problems of environmental pollution and waste of resources, and achieve the goal of no environmental pollution, low cost, and obvious lure effect Effect

Inactive Publication Date: 2010-11-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] my country is a big beer producing country, and most of the discarded beer yeast sludge is directly discharged into the sewer every year, which not only pollutes the environment, but also wastes resources

Method used

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  • Citrus fruit fly protein bait, and preparation method and application method thereof
  • Citrus fruit fly protein bait, and preparation method and application method thereof
  • Citrus fruit fly protein bait, and preparation method and application method thereof

Examples

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Effect test

example 1、1

[0039] Example 1, 1) preparation of brewer's yeast hydrolyzate:

[0040] a. Inactivation: Pasteurization is used to pasteurize the active brewer's yeast slurry at 65°C and cool it down to room temperature naturally.

[0041] b. Filtration: first filter with 80 mesh screen, and then filter with 100 mesh screen to remove impurities in beer yeast mud,

[0042] c. Concentrate by heating: heat and boil on a magnetic stirrer for 2 hours, then naturally cool to room temperature,

[0043] d. Proteolysis: add distilled water to the concentrated yeast liquid to prepare a yeast liquid substrate with a yeast mass fraction of 40%-50%, add 0.12g-0.24g of papain in every 100ml of yeast liquid substrate, stir and mix, Use NaOH and HCl to adjust the pH value to 6.5, set the temperature to 65°C, keep warm in a water bath, and carry out the enzymatic hydrolysis reaction for 12 hours.

[0044] e. Terminate the enzymatic hydrolysis reaction: after the enzymatic hydrolysis reaction is completed, ...

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PUM

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Abstract

The invention relates to citrus fruit fly protein bait, and a preparation method and a application method thereof. The protein bait is prepared from beer yeast hydrolysate, ammonium acetate, brown sugar and orange juice. The preparation method comprises the following steps: carrying out sterilization, filtration and heat concentration on beer yeasts with the activity; heating and boiling the materials on a magnetic stirring device; then, naturally tooling the materials to room temperature; adding distilled water into yeast liquid to be prepared into yeast liquid substrates; adding papain for zymohydrolysis; adding preservatives; and preparing the citrus fruit fly protein bait through mixing the beer yeast hydrolysate, the ammonium acetate, the brown sugar and the orange juice. The use method comprises the step of placing the citrus fruit fly protein bait onto a trap to trap and kill perfect insects or using the citrus fruit fly protein bait for point spraying in orangery. The invention has the advantages of wide raw material sources, simple process, no environment pollution and low cost. The invention uses the condition that the citrus fruit fly perfect insects need the protein supplementation after the eclosion, the ammonium acetate, the brown sugar, the orange juice attrahent and a small amount of pesticide are added in the beer yeast hydrolysate, both male and female citrus fruit fly perfect insects can be killed, and the average attraction rate is 61.11 percent, and is higher than that of American torula and Chinese sweet and sour wine liquor.

Description

technical field [0001] The invention relates to a protein bait for Bactrocera citrus and its preparation method and use method. Background technique [0002] The citrus fruit fly, Bactrocera minax, belongs to Diptera, Tephritidae, and Bactrocera genus. It mainly feeds on citrus plants of Rutaceae and is an important pest of citrus fruit trees. One generation occurs a year, and the adults begin to emerge from the end of April to the beginning of May each year, peak in mid-May, start to lay eggs in mid-June, and continue until late July. The female adults lay eggs in the fruit, causing the fruit to yellow and drop before it is ripe. After hatching, the larvae eat the fruit and cause harm to the fruit, which completely loses its edible value. In recent years, due to climate change, adjustment of planting structure and frequent trade, Bactrocera citrus has become a frequent pest in citrus orchards. Affected farmers to increase production and income. [0003] At present, the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N37/02A01P19/00
Inventor 牛长缨周晓渭韩鹏雷朝亮张宏宇
Owner HUAZHONG AGRI UNIV
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