10 hundred million/g benzimidazoles-resisting bactericide trichoderma conidium granule and preparation method thereof
A technology of benzimidazoles and conidia, applied in the direction of fungicides, botanical equipment and methods, biocides, etc., can solve the problems of unstable control effects in the field, inability to exert biological control effects, sensitivity, etc., and achieve moderate prices , easy to control, and easy to commercialize
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[0024] 1. Production of highly resistant benzimidazole fungicide conidia powder
[0025] 1. First-level slant cultivation
[0026] Trichoderma, which is highly resistant to benzimidazole fungicides, is cultured in PDA medium for 6-7 days.
[0027] 2. Secondary liquid culture
[0028] The above-mentioned culture was inoculated with potato glucose-based liquid for propagation, and cultured with shaking (28±1° C., 200 r / min) for 24 hours, as the seed liquid for solid fermentation.
[0029] 3. Tertiary solid culture
[0030] Soak low-value old rice in boiling water, the best time is 8-10 minutes; then put it into a high-temperature sterilizer for sterilization, the best temperature for sterilization is 105°C, and the best time is 8-10 minutes; When the temperature drops to 25-30°C, put the seed liquid into the culture medium of low-value aged rice, the optimum ratio of seed liquid to rice is 1:10, put it in the incubator for cultivation, the optimum temperature is 28°C, 12 hour...
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