10 hundred million/g benzimidazoles-resisting bactericide trichoderma conidium granule and preparation method thereof

A technology of benzimidazoles and conidia, applied in the direction of fungicides, botanical equipment and methods, biocides, etc., can solve the problems of unstable control effects in the field, inability to exert biological control effects, sensitivity, etc., and achieve moderate prices , easy to control, and easy to commercialize

Inactive Publication Date: 2010-11-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Trichoderma grows fast, produces a large amount of spores, and inhibits a variety of plant pathogens, but is sensitive to benzimidazole fungicides, and cannot play a biological control role when these fungicides are applied in the field
At present, the use of biocontrol factors alone to control crop diseases is often disturbed by environmental conditions and other factors, resulting in unstable control effects in the field. Due to the long-standing problem of sensitivity to chemical pesticides, they cannot be used simultaneously with chemical pesticides. Integrated Control of Plant Diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 1. Production of highly resistant benzimidazole fungicide conidia powder

[0025] 1. First-level slant cultivation

[0026] Trichoderma, which is highly resistant to benzimidazole fungicides, is cultured in PDA medium for 6-7 days.

[0027] 2. Secondary liquid culture

[0028] The above-mentioned culture was inoculated with potato glucose-based liquid for propagation, and cultured with shaking (28±1° C., 200 r / min) for 24 hours, as the seed liquid for solid fermentation.

[0029] 3. Tertiary solid culture

[0030] Soak low-value old rice in boiling water, the best time is 8-10 minutes; then put it into a high-temperature sterilizer for sterilization, the best temperature for sterilization is 105°C, and the best time is 8-10 minutes; When the temperature drops to 25-30°C, put the seed liquid into the culture medium of low-value aged rice, the optimum ratio of seed liquid to rice is 1:10, put it in the incubator for cultivation, the optimum temperature is 28°C, 12 hour...

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Abstract

The invention relates to 10 hundred million / g benzimidazoles-resisting bactericide trichoderma conidium granule and a preparation method thereof, belonging to the technical field of biopesticide and realizing commercialized production of benzimidazoles-resisting bactericide trichoderma. The technical content is as follows: 1, preparation of conidium powder; 2, trichoderma conidium granule and production process; and 3, a comprehensive preventing and treating method of the formulation and the benzimidazoles bactericide applied to plant disease. The granule is beneficial to preventing and treating the disease and reducing the use amount of chemical agent, has the preventing and treating effect of above 80% in the test for preventing and treating the soil-borne diseases such as blight, pythium debaryanum and droop of various field crops and vegetables, is environmentally-friendly and harmless to people and livestock, is low in the investment and easy to be commercialized, has convenient use, long shelf life and a spore germination rate of above 80% in a quality guarantee period of one year, and has the characteristics of long duration, obvious function of increase production, little use amount, low chemical pesticide residual amount, proper price, and the like.

Description

technical field [0001] The invention relates to microbial pesticides, in particular to preparation of trichoderma spore granules containing anti-benzimidazole fungicides and a production process. Background technique [0002] Trichoderma grows fast, produces large spores, and inhibits a variety of plant pathogens. However, it is sensitive to benzimidazole fungicides and cannot play a biological control role when these fungicides are applied in the field. At present, the use of biocontrol factors alone to control crop diseases is often disturbed by environmental conditions and other factors, resulting in unstable control effects in the field. Due to the long-standing problem of sensitivity to chemical pesticides, they cannot be used simultaneously with chemical pesticides. Integrated management of plant diseases. Therefore, in order to solve the problems faced in the application of biocontrol bacteria, realize the combined use of chemical pesticides and Trichoderma, improve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/12A01N47/18A01P3/00
Inventor 杨谦宋金柱周传余张金栋
Owner HARBIN INST OF TECH
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