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58 results about "Benzimidazole fungicide" patented technology

Benzimidazole fungicides are a class of fungicides including benomyl, carbendazim (MBC), thiophanate-methyl, thiabendazole and fuberidazole. They can control many ascomycetes and basidiomycetes, but not oomycetes. They are applied to cereals, fruits, vegetables and vines, and are also used in postharvest handling of crops.

Method for treating drug resistant fusarium blight

InactiveCN1653903AMeet environmental quality requirementsMature production processBiocideAnimal repellantsSaflufenacilHordeum vulgare
The present invention is method of controlling bakanae disease of wheat, barley, etc. with resistance to benzimidazole and similar germicides. Tebuconazole, nuarimol, imazalil, etc. are used alone or compounded, or the preparation with tebuconazole, nuarimol, imazalil, etc. as effective components is re-compounded with other systematic one protective germicide to form binary or polynary preparation for treating seed or field spraying. The present invention has obvious synergistic effect in preventing and treating bakanae disease of wheat, barley, etc. and can delay the generation of chemical resistance.
Owner:NANJING AGRICULTURAL UNIVERSITY

Composition of prohexadione calcium and fungicide

The invention discloses a composition of prohexadione calcium and a fungicide. The composition includes 5 to 50 parts by weight of the prohexadione calcium and 5 to 60 parts by weight of the fungicide; the bactericide is a triazole fungicide such as epoxiconazole, tebuconazole, hexaconazole and difenoconazole, or a carboxylic acid amide fungicide such as thifluzamide and fenoxanil, or a benzimidazole fungicide such as carbendazim and thiophanate-methyl, or an imidazole fungicide such as prochloraz and prochloraz-manganese chloride complex. The composition of the prohexadione calcium and the fungicide provided by the invention can achieve the purposes of vigorous growth control, disease resistance and yield increase, has no effect on next-stubble crops, reduces the pesticide costs of farmers, and increases the crop yield, so as to increase the income of farmers, and create enormous economic and social values.
Owner:郑州郑氏化工产品有限公司

Method for preventing and controlling wheat bakanae disease by JS399-19 and tebuconazole

The present invention is method of using the mixture of JS399-19 and tebuconazole in preventing and controlling bakanae disease of wheat, barley, etc. with resistance to benzimidazole germicides. The present invention features that JS399-19 and tebuconazole in the effective component weight ratio of 1-40 to 1 are compounded, and the compounded preparation is used in preventing and controlling bakanae disease of wheat, barley, etc. through seed treatment and field spraying.
Owner:NANJING AGRICULTURAL UNIVERSITY

Combined seedling root wetting agent and preparation method thereof

The invention provides a combined seedling root wetting agent and belongs to the technical field of wetting agents. The wetting agent is prepared from the following ingredients: 0.5-1 part of rootone, 3-6 parts of humic acid, 10-15 parts of benzimidazole fungicide, 20-30 parts of super absorbent resin, 10-20 parts of monopotassium phosphate, 100-200 parts of sodium carboxymethylcellulose, 15-30 parts of dyeing agent and 450-900 parts of talcum powder. The invention also provides a preparation method of the combined seedling root wetting agent. The combined seedling root wetting agent not only can be used for links such as artificial planting and transportation of forestation seedlings, is capable of replacing an original measure of dipping roots with sludge, and can be applied to preserving moisture of seedlings and promoting the survival during forestation under a watering-free condition.
Owner:HEBEI ACAD OF FORESTRY SCI

Genotyping detection method of drug resistance to Sanmate of Fusarium graminearum

InactiveCN101988122AMicrobiological testing/measurementSusceptibility/Resistance GeneTyping methods
The invention belongs to a Sanmate resistance genotype molecular detection and SNP typing method of Fusarium graminearum, which can be used for drug resistance monitoring and drug resistance genotype diagnosis to Sanmate and other benzimidazole type bactericides of the Fusarium graminearum which can cause wheat scab. The detection method comprises the following three major steps: (1) extracting DNA of a nuclear genome of a strain to be detected; (2) applying three groups of primer pairs to carry out PCR reaction; and (3) identifying the genotype of the strain with the resistance to the Sanmate according to a PCR product electrophoretogram. By adopting the Tetra-primer ARMS PCR (Tetra-primer amplification refractorymutation system-PCR) technology, the strain with the drug resistance of theFusarium graminearum and the genotype thereof can be fast and accurately detected, and the level of the drug resistance can be further judged. The detection accuracy is above 95%.
Owner:NANJING AGRICULTURAL UNIVERSITY

Device for rapidly detecting benzimidazole fungicide-resistant botrytis cinerea Pers. based on LAMP

The invention discloses a loop-mediated isothermal amplification primer composition for detecting benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The LAMP primer composition consists of the following four primers: an F3, a B3, an FIP and a BIP. The primer composition consists of primer groups designed by mismatching a basic group in a mutation region containing a 198 locus of a botrytis cinerea Pers. beta-tubulin gene; loop-mediated isothermal amplification reaction is used for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The invention further provides a loop-mediated isothermal amplification method for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers. and a kit used thereby.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Application of farnesol as antibacterial synergist in preventing and controlling plant grey mold

The invention relates to the field of plant disease prevention and control, in particular to application of farnesol as an antibacterial synergist in preventing and controlling plant grey mold. Particularly, the antibacterial synergist is applied to preventing and controlling the grey mold with a benzimidazole bactericide or triazole bactericide. The farnesol has the synergistic action to the bactericide, can improve the virulence of the bactericide by being used mixing with the bactericide, improves the sensibility of a drug-resistant strain to the bactericide and the prevention and control effect of the bactericide to the field drug-resistant grey mold, reduces the dosage of the bactericide and provides a new method to development and application of a novel preparation of the existing bactericide. Meanwhile, the farnesol is an extract of natural plants and is environmentally friendly. The invention provides a novel application of the drug, can be used for disease prevention and control in the agricultural field and provides a new way to grey mold field drug-resistant government.
Owner:CHINA AGRI UNIV

Fungicidal Mixtures Of Amidinylphenyl Compounds

InactiveUS20080020999A1BiocideDead animal preservationSterol Biosynthesis PathwayMorpholine
Disclosed are fungicidal mixtures, compositions and methods for controlling plant diseases relating to combinations comprising (a) at least one compound selected from phenylamidines of Formula I, N-oxides, and agriculturally suitable salts thereof (I) wherein A is C3alkylene, optionally substituted with one or two methyl; W is CR5R6R7 or SiR8R9R10; and R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are as defined in the disclosure; and (b) at least one compound selected from alkylenebis(dithiocarbamate) fungicides, compounds acting at the bc1 complex of the fungal mitochondrial respiratory electron transfer site, cymoxanil, compounds acting at the demethylase enzyme of the sterol biosynthesis pathway, morpholine and piperidine compounds that act on the sterol biosynthesis pathway, phenylamide fungicides, pyrimidinone fungicides, chlorothalonil, carboxamides acting at complex II of the fungal mitochondrial respiratory electron transfer site, quinoxyfen, metrafenone, cyflufenamid, cyprodinil, copper compounds, phthalimide fungicides, fosetyl-aluminum, benzimidazole fungicides, cyazofamid, fluazinam, iprovalicarb, propamocarb, validamycin, dichlorophenyl dicarboximide fungicides, zoxamide and dimethomorph, and their agriculturally suitable salts.
Owner:KLAPPROTH MICHAEL CALDWELL +1

Sterilizing and insecticidal composition

The invention discloses a composition which comprises bactericide SYP-1620, bactericide benzimidazoles, insecticide imidacloprid, wherein the weight ratio of the two bactericides (based on active constituents) is 1:50 to 50:1, the weight ratio of the sterilizing active compound and insecticide (based on active constituents) is 1:1 to 50:1. The composition can be prepared into missible oil, wet powder, suspending agent and wet pelletized granule. The composition can realize better sterilizing effect, and can be used for controlling aphid, striatellus, Subulatus bipunctatus, Bemisia inconspicua, Lindeman and atatomidae.
Owner:SHENYANG SINOCHEM AGROCHEMICALS R&D CO LTD

Fungicidal mixtures of amidinylphenyl compounds

InactiveCN1960632ABiocideDead animal preservationSterol Biosynthesis PathwayMorpholine
Disclosed are fungicidal mixtures, compositions and methods for controlling plant diseases relating to combinations comprising (a) at least one compound selected from phenylamidines of Formula I, N-oxides, and agriculturally suitable salts thereof (I) wherein A is C3alkylene, optionally substituted with one or two methyl; W is CR<5>R<6>R<7>or SiR<8>R<9>R<10>; and R<1>, R<2>, R<3>, R<4>, R<5>, R<6>, R<7>, R<8>, R<9> and R<10>are as defined in the disclosure; and (b) at least one compound selected from alkylenebis(dithiocarbamate) fungicides, compounds acting at the bc1complex of the fungal mitochondrial respiratory electron transfer site, cymoxanil, compounds acting at the demethylase enzyme of the sterol biosynthesis pathway, morpholine and piperidine compounds that act on the sterol biosynthesis pathway, phenylamide fungicides, pyrimidinone fungicides, chlorothalonil, carboxamides acting at complex II of the fungal mitochondrial respiratory electron transfer site, quinoxyfen, metrafenone, cyflufenamid, cyprodinil, copper compounds, phthalimide fungicides, fosetyl-aluminum, benzimidazole fungicides, cyazofamid, fluazinam, iprovalicarb, propamocarb, validamycin, dichlorophenyl dicarboximide fungicides, zoxamide and dimethomorph, and their agriculturally suitable salts.
Owner:EI DU PONT DE NEMOURS & CO

10 hundred million/g benzimidazoles-resisting bactericide trichoderma conidium granule and preparation method thereof

The invention relates to 10 hundred million / g benzimidazoles-resisting bactericide trichoderma conidium granule and a preparation method thereof, belonging to the technical field of biopesticide and realizing commercialized production of benzimidazoles-resisting bactericide trichoderma. The technical content is as follows: 1, preparation of conidium powder; 2, trichoderma conidium granule and production process; and 3, a comprehensive preventing and treating method of the formulation and the benzimidazoles bactericide applied to plant disease. The granule is beneficial to preventing and treating the disease and reducing the use amount of chemical agent, has the preventing and treating effect of above 80% in the test for preventing and treating the soil-borne diseases such as blight, pythium debaryanum and droop of various field crops and vegetables, is environmentally-friendly and harmless to people and livestock, is low in the investment and easy to be commercialized, has convenient use, long shelf life and a spore germination rate of above 80% in a quality guarantee period of one year, and has the characteristics of long duration, obvious function of increase production, little use amount, low chemical pesticide residual amount, proper price, and the like.
Owner:HARBIN INST OF TECH

1 billion/gram high-resistance benzimidazole germicide trichoderma conidium wettable powder and preparation method thereof

The invention relates to 1 billion / gram high-resistance benzimidazole germicide trichoderma conidium wettable powder and a preparation method thereof, belonging to the technical field of biopesticides. The 1 billion / gram trichoderma conidium wettable powder comprises the following components: trichoderma conidium, a wetting agent DY-ETE301, sodium lignin sulfonate used as a dispersing agent, riboflavin used as an ultraviolet protective agent, residual rice powder, diatomite, and the like. In the method, a separating type processing technology is used for processing and preparing the wettable powder. The wettable powder can be applied to preventing and treating plant diseases such as gray molds, rhizoctonia rot, damping off, droops, and the like of various field crops and vegetables together with benzimidazole germicides. The prevention and treatment ratio of the wettable powder is higher than 80 percent, and the wettable powder has no environmental pollution, no harm to humans and animals and convenient use; and the quality guarantee period of the wettable powder is one year, and the spore germination ratio is higher than 80 percent. Meanwhile, the wettable powder has the characteristics of long efficacy duration, obvious yield increasing effect, small application amount, little chemical pesticide residue, effective environmental pollution reduction, and the like.
Owner:HARBIN INST OF TECH

Method for treating fungal infections, fungicidal compositions and their use

A fungicidal composition and a method for treating plants using the composition are provided. The composition comprises at least three active ingredient components (A), (B) and (C), optionally together with one or more customary formulation auxiliaries, wherein component (A) is one or more triazole fungicides, component (B) is one or more strobilurin fungicides, and component (C) is one or more benzimidazole fungicides. Further provided is a method for preventing and / or combating pathogenic damage or pest damage in a plant or plant material, which comprises applying on the plant, plant material, part of plant, or surroundings thereof the composition comprising the active ingredient components (A), (B) and (C).
Owner:ROTAM AGROCHEM INT CO LTD

Humectant used for dipping root in seedling transplantation, and preparation method thereof

The invention provides a humectant used for dipping root in seedling transplantation, and a preparation method thereof. The humectant comprises, by weight, 15 to 26 parts of carboxymethyl cellulose, 5 to 12 parts of humic acid, 10 to 15 parts of a benzimidazole fungicide, 10 to 20 parts of potassium dihydrogen phosphate, 15 to 30 parts of a super absorbent resin, 100 to 200 parts of starch grafted sodium acrylate, 100 to 200 parts of sodium naphthalene-1-acetate, 450 to 900 parts of talcum powder, 300 to 600 parts of Streptomyces globisporus fermentation broth, and 200 to 500 parts of water. The humectant is capable of promoting healing of seedling root wounds, and rooting, and increasing survival rate in seedling transportation process.
Owner:HFEI PUBANG AGRI TECH CO LTD

Detecting gene of bacillus anthracis anticarbendazol and its reagent box

A gene for testing the resistance of colletotrichum gloeosporiodies to carbendazim and its test reagent kit are disclosed. Said gene is the beta-microtubulin gene fragment of Colletotrichum gloeosporiodies. Said reagent kit is composed of 10XPCR buffering liquid, MgCl2 solution, dNTP (Mixture), upstream primers CGR1 and CGS2, and downstream primers CGrev and TaqDNA polymerase. Its advantages are high speed, high correctness (95.71%) and high sensitivity.
Owner:NANJING AGRICULTURAL UNIVERSITY

Test kit for detecting drug resistance of botrytis cinerea to benzimidazole fungicides

InactiveCN108018374AConvenient and sensitive detection of drug resistanceMeet testing needsMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceMolecular level
The invention provides a test kit for detecting drug resistance of botrytis cinerea to benzimidazole fungicides. The kit comprises a primer combination. The primer combination comprises a primer set I, a primer set II, and / or a primer set III; the primer set I consists of Tub-F3, Tub-B3, Tub-FIP-E198A and Tub-BIP; the primer set II consists of Tub-F3, Tub-B3, Tub-FIP-E198V, and Tub-BIP; the primerset III consists of Tub-F3, Tub-B3, Tub-FIP-E198K, and Tub-BIP. The invention provides a convenient and flexible method for detecting the drug resistance of the botrytis cinerea with direct judgmentresults and capable of being operated in the field based on a molecular level, and can ensure a primary technician to obtain objective and reliable detection results in the field within two hours.
Owner:HUAZHONG AGRI UNIV

Propineb and iprodione-containing antiseptic composition

The invention provides a propineb and iprodione-containing antiseptic composition. The active ingredients of the propineb and iprodione-containing antiseptic composition comprise propineb and iprodione in mass percent ratio of 10-80:40-1. Bioassay tests show that with different action mechanisms, the propineb and the iprodione, after being combined, do not generate cross-resistance, have obvious synergized action and can delay the generation of drug resistance of benzimidazole antiseptics. The composition is mainly used for preventing and controlling sigatoka, apple leaf spot disease, altemaria mali Roberts and early blight of tomatoes.
Owner:SHAANXI MEIBANG PHARMA GRP CO LTD

Application of reserpine as fungicidal synergist to prevention and control of plant grey mould

The invention relates to the field of prevention and control of plant diseases, and in particular to application of reserpine as a fungicidal synergist to prevention and control of plant grey mould. Specifically, the fungicidal synergist is applied along with methoxyacrylate fungicide, nicotinamide fungicide, benzimidazole fungicide, triazole fungicide, anilinopyrimidine fungicide, imide fungicide, pyrrole fungicide or substituted aniline fungicide in the prevention and control of the grey mould. Reserpine has a synergistic effect on the fungicide, and when being mixed with the fungicide for use, reserpine can improve the toxicity of the fungicide, improve the sensitivity of resistant strains to the fungicide, improve the prevention and control effect of the fungicide on the grey mould already with resistance in fields and reduce the dosage of the fungicide, thus providing a novel method for the development and application of novel preparations of conventional fungicides. Moreover, reserpine is a natural plant extract, and therefore is environment-friendly. The invention provides new application of such chemicals, the chemicals can be used for preventing and controlling diseases inthe field of agriculture, and a new approach is provided for the control of grey mould resistance in fields.
Owner:CHINA AGRI UNIV

Effect-increased bactericide composition, preparation method and application thereof

The invention aims to disclose an effect-increased bactericide composition. Raw materials of the bactericide composition comprise, by weight, 1-7 parts of metconazole, 1-7 parts of thiram and 0.5-2 parts of an effect-increasing agent. The effect-increasing agent is one or more of humic acid, magnesium humate, zinc humate and potassium humate. A preparation method includes following steps: adding conventional auxiliary materials to the raw materials and preparing a powdery agent, which is acceptable in use, according to conventional processes. The invention also discloses applications of the bactericide composition in prevention and cure of wheat scab pathogens, powdery mildew or rust, and particularly discloses an application of the bactericide composition in prevention and cure of wheat scab pathogens which has drug resistance to a benzimidazole bactericide. With addition of the effect-increasing agent, the bactericide composition not only achieves maximum effect in bactericidal activity but also has obvious and remarkable growth-stimulating and production-increasing effects.
Owner:南京南农农药科技发展有限公司

Bactericidal composition containing coumoxystrobin and benzimidazole bactericide

The invention discloses a bactericidal composition containing coumoxystrobin and benzimidazole bactericide. The bactericidal composition comprises the coumoxystrobin serving as an active ingredient and the benzimidazole bactericide selected from thiophanatemethyl, carbendazol or benomyl; and the weight ratio of the coumoxystrobin to the benzimidazole bactericide is 1:1-1:50. The composition can be prepared into agriculturally allowable water dispersible granules, wettable powder or suspension. The composition has reasonable components, good bactericidal effect and low application cost, and the activity and the bactericidal effect of the composition are not simply superposed activity and bactericidal effect of the components; compared with the conventional single preparation, the composition has the advantages of remarkable bactericidal effect, remarkable synergy, retarded resistance, low preparation residue, no pollution to crops, good safety and accordance with the safety requirement of the pesticide preparation; and the composition has remarkable effect of preventing and controlling apple ring rot, anthracnose, rot, rice sheath blight, rice blast and wheat scab.
Owner:SHAANXI SUNGER ROAD BIO SCI

Novel environment-friendly bactericidal composition

The invention relates to a bactericidal composition preparation which belongs to the field of the compounding of pesticides. The preparation is obtained by the binary blending of cyflufenamid and partial benzimidazole bactericides which are taken as active ingredients, wherein the weight ratio of the cyflufenamid to the partial benzimidazole bactericides is (80:1)-(1:90), (50:3)-(5:65) preferably; and the total weight content of the active ingredients in the bactericidal composition is 1 to 90 percent, 8 to 75 percent preferably. The balance is auxiliary ingredients which are allowed and acceptable in the pesticides. The preparation can be prepared into wettable powder, a suspending agent, soluble liquid, emulsion in water and water dispersible granules by a known method. The bactericidal composition is mainly used for controlling fungal diseases of crops such as grains, cotton, oil, fruits, vegetables, flowers and the like.
Owner:SHANGDONG HAILIER CHEM

Rubber tree colletotrichum drug-resistant strain HcgHNQZ1736 and application thereof in drug resistance research

The invention belongs to the technical field of plant genetic engineering, and particularly relates to karst colletotrichum and application thereof in the field of rubber anthracnose research. The karst colletotrichum is found on rubber trees in Hainan Province for the first time; and a strain obtained through separation is C.karstii. Biological characteristic determination finds that the optimumgrowth temperature of the strain is 28 DEG C; the lethal temperature is 35 DEG C; the optimum growth pH is 6; light and dark alternation is beneficial to colony growth; and pectin and peptone are respectively an optimal carbon source and an optimal nitrogen source. Pathogenicity evaluation finds that the strain has relatively strong pathogenicity on four main recommended varieties including PR107,RRIM600, hot research 7-33-97 and Dafeng 95; a drug resistance evaluation result shows that the strain HcgHNQZ1736 has no drug resistance to a sterol demethylation inhibitor (DMIs) bactericide prochloraz-manganese chloride complex, and has high drug resistance to a benzimidazole bactericide carbendazim.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Compound bactericide and preparation method thereof

InactiveCN104542658AMany mechanisms of actionMany sitesBiocideFungicidesPlant diseaseBiology
The invention discloses a compound bactericide and a preparation method thereof. The compound bactericide disclosed by the invention is composed of an active component, a filling material and an assistant agent, wherein the active component is composed of triazole bactericide tebuconazole and benzimidazole bactericide carbendazim; and the compound bactericide is capable of effectively preventing and treating branch fungal diseases, such as valsa ceratosperma and macrophoma kawatsukai. The compound bactericide disclosed by the invention has the obvious synergistic effect of preventing and treating valsa ceratosperma fungal diseases; the removing effect of X% of DW paste is up to 100% within 120 days; the effect is obviously higher than that of single preparation; the compound bactericide has good fast-acting property and good durable effect and more action mechanisms and acting sites, and is capable of effectively delaying generation and development of drug resistance of pathogenic bacteria; and in addition, the compound bactericide disclosed by the invention adopts a simple preparation process, and is low in cost, obvious in economic benefit, wide in disease prevention spectrum and high in biological safety.
Owner:HEBEI AGRICULTURAL UNIV.

Use of polyhexamethylene guanidine hydrochloride as preservative for preventing and controlling citrus sour rot and its application

The use of polyhexamethylene guanidine hydrochloride as preservative for preventing and controlling citrus sour rot and its application are disclosed by the present invention. The citrus preservative calculated by 100% total mass fraction comprises 0.05%-0.5% polyhexamethylene guanidine hydrochloride, 0.05%-0.2% of one or more selected from the group consisting of a mixture of several of the following: 0.05%-0.2% imidazole fungicidebactericide, benzimidazole fungicidebactericide, pyrimethanil, fludioxonil and [or] azoxystrobin, and 0.02%-0.15% polyethenoxy ether emulsion or quaternary ammonium salt emulsion, and the rest is water. The citrus preservative can significantly inhibit the decay of citrus fruit such as Citrus microcarpa during storage, especially the incidence of sour rot. So the commercial value of citrus is obviously increased.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Insect-resistant root dipping agent for gingko and preparation method thereof

The invention discloses an insect-resistant root dipping agent for gingko and belongs to the technical field of seedling root dipping agents. The insect-resistant root dipping agent contains the following components in parts by weight: 10-20 parts of an organophosphorus insecticide, 10-15 parts of a benzimidazole fungicide, 1-2 parts of a GGR6# rooting agent, 20-30 parts of a super absorbent resin, 100-200 parts of sodium carboxymethylcellulose and 500-1000 parts of talcum powder. According to the insect-resistant root dipping agent for gingko, disclosed by the invention, on one hand, a mud layer formed on the surface of the seedling root can protect the root system, and an effect of moisturizing the roots is achieved; on the other hand, hazards of soil insects such as grubs, mole cricketsand wireworms can be prevented and alleviated, and root system growth is facilitated, so that the transplanting survival rate and retention rate of gingko seedlings are improved. Due to the organic combination of seedling mud dipping and control of plant diseases and insect pests, the transplanting survival rate of the gingko seedlings is improved.
Owner:HEBEI ACAD OF FORESTRY SCI

Use of polyhexamethylene guanidine hydrochloride as preservative to control citrus sour rot and its application

The use of polyhexamethylene guanidine hydrochloride as preservative for preventing and controlling citrus sour rot and its application are disclosed by the present invention. The citrus preservative calculated by 100% total mass fraction comprises 0.05%-0.5% polyhexamethylene guanidine hydrochloride, 0.05%-0.2% of one or more selected from the group consisting of a mixture of several of the following: 0.05%-0.2% imidazole fungicidebactericide, benzimidazole fungicidebactericide, pyrimethanil, fludioxonil and azoxystrobin, and 0.02%-0.15% polyethenoxy ether emulsion or quaternary ammonium salt emulsion, and the rest is water. The citrus preservative can significantly inhibit the decay of citrus fruit such as Citrus microcarpa during storage, especially the incidence of sour rot. So the commercial value of citrus is obviously increased.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Detection method of colletotrichum gloeosporioides penz drug-resistant strain to benzimidazoles bactericide

The invention relates to a detection method of a colletotrichum gloeosporioides penz drug-resistant strain to benzimidazoles bactericide, comprising the following four steps of: (1) extracting a colletotrichum gloeosporioides penz genomic DNA; (2) carrying out PCR (Polymerase Chain Reaction) amplification on a specific primer under certain reaction conditions; (3) carrying out enzyme cutting on a PCR amplified product; and (4) analyzing an enzyme-cutting product, wherein the detection method has 100 percent of detectable rate on the colletotrichum gloeosporioides drug-resistant strain. In the invention, the DNA segment (includes a specific restriction enzyme cutting site) amplification is carried out on a specific primer pair selected according to the specific restriction enzyme cutting site of a pathogenic bacteria resistant strain, the enzyme cutting is carried out on the amplified DNA segment by using a restriction endonuclease, and the enzyme-cutting is subjected to electrophoresis and gel imaging so as to accurately judge whether the pathogenic bacterium is a resistant strain or a sensitive strain. The method is proved to be stable and reliable through multiple tests.
Owner:SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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