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Antifungal lipopeptid-containing skin liniment and preparation method thereof

An antifungal, lipopeptide technology, applied in antifungal agents, medical preparations containing active ingredients, microorganism-based methods, etc., can solve problems such as drug resistance of pathogenic fungi

Inactive Publication Date: 2012-05-02
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current medicine for the treatment of skin ringworm is the ointment containing ketoconazole or clotrimazole, which belongs to chemically synthesized drugs, and long-term use will lead to drug resistance of pathogenic fungi

Method used

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  • Antifungal lipopeptid-containing skin liniment and preparation method thereof
  • Antifungal lipopeptid-containing skin liniment and preparation method thereof
  • Antifungal lipopeptid-containing skin liniment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (1) Cultivation of microbial strains and fermentation of antifungal lipopeptides

[0079] Composition of slant and seed medium: (g / L): glucose 3, tryptone 5, yeast extract powder 6, NaCl 6, deionized water preparation, 7.5; solid medium needs to add 15g agar powder.

[0080] Composition of fermentation medium (g / L): 5 g of sugary material (glucose), 15 g of nitrogen source (ammonium nitrate), 1 g of sodium citrate, MgSO 4 ·7H 2 00.5g, K 2 HPO 4 2~6g, KH 2 PO 4 5g, pH 7.0-7.2. The sterilization temperature of seed medium and fermentation medium is 115, 20min.

[0081] (2) The strain BI1 was inoculated from a slant into a 250 mL Erlenmeyer flask containing 50 mL of seed medium, and cultured on a shaker at 40° C. and 140 r / min for 16 hours. Insert the cultivated seeds into the fermentation medium according to the inoculation amount of 2% (v / v), the cultivation temperature is 40°C, and the fermentation ventilation volume is 8m 3 / h, tank pressure 0.05MPa, stirring s...

Embodiment 2

[0085] (1) The fermentation strain used is CGMCC No.3412, and the composition of the fermentation medium is (g / L): glucose 5g, nitrogen source ammonium sulfate 2g, sodium citrate 3g, MgSO 4 ·7H 2 00.2g, K 2 HPO 4 2g, KH 2 PO 4 3g, pH 7.0;

[0086] (2) Fermentation culture: Insert the cultured seeds into the fermentation medium at an inoculum size of 10% (v / v), the culture temperature is 30°C, and the fermentation ventilation volume is 13m 3 / h, tank pressure 0.03MPa, stirring speed 120 rpm, fermentation time 30h, feed sterile air during fermentation, maintain the pH of the fermentation broth at 7.5 with ammonia water, and sterilize the seed medium and fermentation medium at 121 ℃, 20min. The output of antifungal lipopeptide in the fermentation broth was 1.5g / L.

[0087] (3) Further control the temperature of the fermentation broth at 4°C, 10000r / min, centrifuge for 20min to remove bacteria, adjust the pH of the obtained sterilized fermentation broth to 2.0 with 6mol / L H...

Embodiment 3

[0090] (1) The fermentation strain used is CGMCC No.3412, and the composition of the fermentation medium is (g / L): corn flour saccharification liquid 3.5 nitrogen source peptone 15, sodium citrate 1, MgSO 4 ·7H 2 00.8,K 2 HPO 4 4. KH 2 PO 4 6, pH 7.2.

[0091] (2) Fermentation culture: Insert the cultured seeds into the fermentation medium at an inoculum size of 2% (v / v), the culture temperature is 30°C, and the fermentation ventilation volume is 8m 3 / h, tank pressure 0.05Pa, stirring speed 150 rpm, fermentation time 60h, feed sterile air during the fermentation process, maintain the pH of the fermentation broth with ammonia water at 7.2, and the output of antifungal lipopeptide in the fermentation broth is 1.4g / L.

[0092] (3) Further control the temperature of the fermentation broth at 4°C, 10000r / min, centrifuge for 20min to remove bacteria, adjust the pH of the obtained sterilized fermentation broth to 2.0 with 6mol / L HCl, and let it stand overnight at 4°C; the next ...

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Abstract

The invention relates to antifungal lipopeptid produced by microbial fermentation and an antifungal lipopeptid-containing skin liniment, belongs to the technical field of biology, and in particular relates to microbial bacillus subtilis BI1 with the preservation number of CGMCC No.3412 for producing the antifungal lipopeptid by using a fermentable sugar raw material. The invention also relates to an antifungal lipopeptid extract produced by microbial fermentation, and an extract-containing film coating agent and a preparation method thereof at the same time. When the film coating agent of the invention is used for treating dermatophytosis, the average cure time is 7 days and the cure rate reaches 100 percent, so a good therapeutic medicament for treating the dermatophytosis is provided for patients.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a strain for producing antifungal lipopeptide by microbial fermentation, and a method for preparing skin liniment by extracting the antifungal lipopeptide. The strain is a new strain found in the present invention that can effectively produce the antifungal lipopeptide, and the antifungal lipopeptide fermented by the strain can be effectively used for treating skin ringworm. Background technique [0002] Lipopeptide is a secondary metabolite produced by Gram-positive bacillus. The molecule of lipopeptide is composed of two parts: hydrophilic peptide bond and lipophilic aliphatic hydrocarbon chain. Due to its special chemical composition and amphiphilic molecular structure, it has the characteristics of biosurfactant. It is widely used in medicine, agriculture, food and cosmetics. It has important application prospects in fields such as oil extraction and environmental governan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00A61K38/16A61K9/00A61K9/06A61P17/00A61P31/10C12R1/125
Inventor 王德培徐进
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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