Method for fermenting animal edible lactobacillus

Abstract

The invention provides a fermentation method applied to feed lactobacillus, which comprises the following steps of: (1) preparing materials, namely adding the following components in percentage by weight based on the overall weight of a culture medium: 50 percent of soybean pulp, 33 percent of wheat bran, 16 percent of corn and 1.0 percent of microelement; (2) crushing and grading; (3) performing high-temperature steam sterilizing treatment on the materials before fermentation; (4) adding water for stirring; and (5) fermenting, namely keeping the temperature at 40 DEG C and fermenting for 48 hours. The fermentation method has the advantages that: 1, most raw materials of the formula are raw materials of the feed, so that the microorganism culture condition completely meets in vivo conditions of animals, and the microorganism nutrition modeling completely adapts to animal in vivo growth environment; 2; the raw materials have wide sources and low cost; 3, the formula is simple, has only three or four raw materials, but can fully meet the microorganism growth nutrition requirement; 4, the same formula is effective for liquid culture and solid culture; and 5, the bacteria concentration of the fermentation product of beneficial bacteria can easily reach 108 under general culture condition.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for fermenting animal edible lactobacilli. technical background [0002] For the cultivation of lactic acid bacteria for animal consumption, in the past, the production medium formula was mostly based on skim milk and broth, and sugar, citrate, hydrogen phosphate, Tween, etc. were all necessary components. The ingredients necessary for microbial growth are provided in the form of purified proteins, peptides, amino acids, monosaccharides, and disaccharides. The advantages of these formula designs are good microbial growth, clear chemical composition and nutrient composition. [0003] However, an important purpose of using lactic acid bacteria for feeding is to inoculate the intestinal tract with beneficial microorganisms, and it is expected that beneficial lactic acid bacteria can grow well in the intestinal tract of animals. However, the design of tr...

AI Technical Summary

Problems solved by technology

However, the design of traditional culture medium is quite different from the nutritional environment in the intestinal tract of animals. The microorganisms cultured in the above-mentioned medium are not suitable for the material environment in the intestinal tract of animals, and the growth in the animal body also shows the stress of the nutritional environment.

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