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Injectable bone repairing material and preparation method thereof

A bone repair and injection technology, applied in medical science, prosthesis, etc., can solve the problems of fast curing, slow biodegradation, and poor stability of bone repair materials, and achieve good biocompatibility, promote regeneration, and stability Good results

Inactive Publication Date: 2011-01-05
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a bone repair material and its preparation method, which can solve the problems of fast curing speed, poor stability and slow biodegradation of the prior art bone repair materials.

Method used

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  • Injectable bone repairing material and preparation method thereof
  • Injectable bone repairing material and preparation method thereof
  • Injectable bone repairing material and preparation method thereof

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preparation example Construction

[0037] The specific preparation method includes the following steps:

[0038] 1) Use gamma rays to irradiate sodium alginate to obtain sodium alginate with an average molecular weight of 6000-20000, preferably with an average molecular weight of 6000-15000;

[0039] 2) A hydrochloric acid solution with a pH of 4-4.5 prepared with pure water and hydrochloric acid;

[0040] 3) Dissolve the sodium alginate prepared in step 1 in the hydrochloric acid solution prepared in step 2. The mass percentage of sodium alginate to the volume percentage of hydrochloric acid is preferably 0.5-1%, and a certain amount of adipic acid dihydrazide ( adipic dihydrazide, abbreviated as ADH), the mass percentage concentration of ADH is 0.5-20%, and the ratio of ADH to alginic acid sugar units is preferably 1:1-1:5, preferably, the mass ratio of sodium alginate to ADH is 1 :15-1:20;

[0041] 4) Combine a certain quality of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxybenzotr...

Embodiment 1

[0059] First, prepare AAlg-g-PNIPAAm of formula (I) according to reaction formula (II), and the specific preparation method is as follows:

[0060] 1) Use gamma rays to irradiate sodium alginate to obtain low molecular weight sodium alginate with an average molecular weight of 6000-8000;

[0061] 2) A hydrochloric acid solution with a pH of 4-4.5 prepared with pure water and hydrochloric acid;

[0062] 3) Dissolve 1 g of sodium alginate prepared in step 1 in 100 ml of hydrochloric acid solution prepared in step 2, and add 15 g of ADH after complete dissolution;

[0063] 4) Dissolve 1.6g EDC and 1.4g HOBt in dimethyl sulfoxide (DMSO) / water solution, the concentration of EDC and HOBt are 10mmol / ml respectively, the ratio of DMSO and water is 1:1, add this solution to In the mixed solution of step 3, stir and react at room temperature for 24 hours;

[0064] 5) Dialysis the reaction solution of step 4 in pure water for 3 days, the molecular weight of the dialysis bag semipermeable membrane...

Embodiment 2

[0072] First, prepare AAlg-g-PNIPAAm of formula (I) according to reaction formula (II), and the specific preparation method is as follows:

[0073] 1) Use gamma rays to irradiate sodium alginate to obtain low molecular weight sodium alginate with an average molecular weight of 6000-8000;

[0074] 2) A hydrochloric acid solution with a pH of 4-4.5 prepared with pure water and hydrochloric acid;

[0075] 3) Dissolve 0.5 g of sodium alginate prepared in step 1 in the 100 ml hydrochloric acid solution prepared in step 2, and add 10 g of ADH after it is completely dissolved;

[0076] 4) Dissolve 0.8g EDC and 0.7g HOBt in DMSO / water solution, where the ratio of DMSO to water is 1:1, add the solution to the mixed solution in step 3, and stir for 24 hours at room temperature;

[0077] 5) Dialysis the reaction solution of step 4 in pure water for 3 days, the molecular weight of the dialysis bag semipermeable membrane can dialyze out is 6000, then take out the dialysis solution, add a certain amo...

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Abstract

The invention relates to an injectable bone repairing material and a preparation method thereof. The injectable bone repairing material mainly comprises poly(N-isopropylacrylamide)-grafted solution of aminated alginic acid. The method comprises the following steps of: preparing poly(N-isopropylacrylamide)-grafted aminated alginic acid; dissolving the poly(N-isopropylacrylamide)-grafted aminated alginic acid in buffer solution of phosphoric acid; adding one or more components selected from hydroxyapatite, beta-tricalcium phosphate, cells and bone growth factors into the prepared buffer solution of the phosphoric acid and uniformly stirring the mixture; and filling the obtained product into a syringe for later use. The injectable bone repairing material has the characteristics of body temperature sensitivity, high plasticity, high degradability, capability of promoting the regeneration of bone defect, high stability, high safety and the like.

Description

Technical field [0001] The invention belongs to the field of biologically active materials, in particular to an injectable bone repair material and a preparation method thereof. Background technique [0002] The development of minimally invasive technology makes injectable bone repair materials an important research field in the field of biomaterials. The injectable bone repair material can be plastic at will; it can be solidified and formed in situ to fill bone defects of various complex shapes; shorten the surgical operation time; have the advantages of less trauma and low cost. In addition, injectable materials can also be loaded with cells, growth factors and genes. Among injectable repair materials, hydrogel has been widely studied and applied because of its biomimetic structure like extracellular matrix and good biocompatibility. Alginic acid is a kind of natural polymer used to prepare injectable repair materials. The chemical composition of alginic acid is β-D-mannosyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/38A61L27/54A61L27/50C08B37/04C08G81/02
Inventor 佘振定刘伟强谭荣伟王明波黄伟廖振华李丽花李小丽张文强王臻
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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