Pseudoginsenoside G1 and extraction method and medicinal applications thereof

A technology of pseudo-ginsenosides and extraction methods, applied in the field of pseudo-ginsenosides G1 and its extraction, can solve the problems of high cost and unsuitable for surgery, reduce content, alleviate ischemic electrocardiogram changes, and reduce platelet adhesion and aggregation sexual effect

Active Publication Date: 2011-01-19
吉林圣亚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the coronary artery disease is scattered, it is not suitable for surgery. In addition, surgical treatment is more expensive than drug treatment, and drug maintenance treatment is still required after surgery.

Method used

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  • Pseudoginsenoside G1 and extraction method and medicinal applications thereof
  • Pseudoginsenoside G1 and extraction method and medicinal applications thereof
  • Pseudoginsenoside G1 and extraction method and medicinal applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 5.0 kg of American red ginseng, pulverize it, add 3 times the amount of 70% ethanol to heat and reflux for extraction, and extract 6 times in total for 4 hours each time. Remover: CHCl 3 -MeOH 80:20; C-18 reverse phase column chromatography, eluent: MeOH-H 2 O 50:30; Methanol-water 1:2 recrystallization to obtain pseudo-ginsenoside G 1 .

[0024] Identification:

[0025] White powder, Liebermann-Burchard reaction and Molish reaction are all positive, indicating that it is a triterpenoid saponin compound.

[0026] The structure was identified by spectroscopic methods.

[0027] 1 H-NMR spectrum (500MHz, C 5 D. 5 No ene hydrogen proton signal is given in N); 8 methyl signals are given at high field: δ1.40(3H, s), 1.37(3H, s), 1.23(3H, s), 1.20(3H, s) , 1.10(3H, s), 1.06(3H, s), 0.80(3H, s), 0.76(3H, s); the middle field gives 14 proton signals of two groups of glucose, including two sugar terminal substrates Proton signals δ4.89 (1H, d, J = 7.5Hz), 5.35 (1H, ...

Embodiment 2

[0033] Take 5.0kg of American red ginseng, pulverize it, add 5 times the amount of 85% ethanol to heat and reflux for extraction, and extract 5 times in total for 3 hours each time. Remover: CHCl 3 -MeOH 100:30; C-18 reverse phase column chromatography, eluent: MeOH-H 2 O 60:40; Methanol-water 1:3 recrystallization to obtain pseudo-ginsenoside G 1 .

Embodiment 3

[0035] Take 5.0 kg of American red ginseng, crush it, add 7 times the amount of 95% ethanol to heat and reflux for extraction, each time for 2 hours, and extract 3 times in total, combine the extracts, and recover the solvent to dryness. The ethanol extract was subjected to silica gel column chromatography, eluent: CHCl 3 -MeOH 110:40; C-18 reverse phase column chromatography, eluent: MeOH-H 2 O 70:50; recrystallization from methanol-water 1:5 to obtain pseudoginsenoside G 1 .

[0036]

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Abstract

The invention relates to a pseudoginsenoside G1 and an extraction method and medicinal applications thereof, belonging to a new compound and medicinal applications thereof. The method comprises the following steps: taking Red American ginseng to grind, adding 3-7 times of 70-95% ethanol to heat and reflux for extraction, wherein each extraction requires 2-4 hours and 3-6 times of extractions are needed; mixing the extracting solutions, recovering the solvent till dry; adopting the silica gel column chromatography to treat ethanol extract, wherein the eluent is CHCl3-MeOH and the weight ratio is 80-110:20-40; adopting the C-18 reversed phase column chromatography to treat, wherein the eluent is MeOH-H2O and the weight ratio is 50-70:30-50; and using the mixture of methanol and water in a weight ratio of 1:2-5 to perform crystallization and obtain the pseudoginsenoside G1. The chemical name of the product is 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-dammarane-12-one-20S,24R-epoxy-3beta,25-diol. The pseudoginsenoside G1 can be used in the preparation of medicines for curing myocardial infarction, resisting myocardial ischemia and reducing the adhesion and aggregation of platelets.

Description

technical field [0001] The invention relates to pseudo-ginsenoside G1 and its extraction method, and its application in the preparation of medicine for treating myocardial ischemia. Background technique [0002] Myocardial ischemia (Heart muscle ischemia, MI) is a clinical common disease, frequently-occurring disease. According to the "China Cardiovascular Disease Report 2006" report, China is in a period of economic transition, the national economy is developing rapidly, and people's lifestyles have undergone major changes. Overall, the life expectancy of the Chinese population has increased, but chronic diseases represented by cardiovascular diseases are increasing day by day, and the age of onset is moving forward. The high morbidity, disability and mortality of cardiovascular diseases have caused heavy burdens on society and residents, and have become a major public health problem. According to the data of the third national health service survey, the prevalence rate o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A61K31/7048A61P9/10A61P7/02
Inventor 李平亚刘金平卢丹刘传贵杜秀娟李丰
Owner 吉林圣亚医药科技有限公司
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