Acetone-butanol in-situ extraction continuous fermentation device and technology

A kind of acetone-butanol fermentation device technology, applied in the field of acetone-butanol in-situ extraction continuous fermentation device, can solve the problems of poor stability, difficulty in large-scale production, high equipment price, etc., and achieve the goals of reduced dosage, easy implementation, and increased output Effect

Inactive Publication Date: 2013-06-05
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor stability and high equipment prices of these technologies in continuous fermentation, it is difficult to be widely used in large-scale production

Method used

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  • Acetone-butanol in-situ extraction continuous fermentation device and technology
  • Acetone-butanol in-situ extraction continuous fermentation device and technology
  • Acetone-butanol in-situ extraction continuous fermentation device and technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) with corn steep liquor as raw material, its content in the culture medium is 20g / L, and the additional nutrient is 0.3g / LCaCl 2 , 0.2g / L MgSO 4 ·7H 2 O, 0.5g / L KH 2 PO4, 0.5g / L urea; configure 0.5M NaOH to inject into the lye tank; oleyl alcohol stock solution is injected into the extractant storage tank for use. Fermentation begins with in-situ steam sterilization of the equipment.

[0025] 2) Preparation of seed liquid: with Clostridium acetobutylicun CGMCCAS1.132 as fermentation strain, (purchased from China Microorganism Preservation and Management Commission Ordinary Microorganisms Collection Center), the component of each liter of seed medium is: 40g glucose , 6g tryptone, 2g yeast extract, 2g beef extract, 3g (NH 4 ) 2 SO 4 , 0.2g MgSO 4 ·7H 2 O, 0.5g KH 2 PO 4 , 0.01g FeSO 4 ·7H 2 O, adjust pH=6.0~6.5. Inject the seed medium into the No. 1 seed fermenter, and the injection amount is 2 / 3 of the volume of the fermenter. 2 Deoxygenate for 2 to 3 ho...

Embodiment 2

[0033] 1) with cassava pulp as raw material, its content in culture medium is 40g / L, and additional nutrient is 0.3g / LCaCl 2 , 0.2g / L MgSO 4 ·7H 2 O, 0.5g / L KH 2 PO4, 0.5g / L urea; configure 0.5M NaOH to inject into the lye tank; oleyl alcohol stock solution is injected into the extractant storage tank for use. Fermentation begins with in-situ steam sterilization of the equipment.

[0034] 2) Preparation of seed liquid: Clostridium acetobutylicunm CGMCCAS1.244 was used as the fermentation strain, (purchased from the General Microorganisms Collection Center of China Microorganism Preservation and Administration Commission).

[0035] The steps were the same as in Example 1, and the incubation temperature was set to 35°C.

[0036] 3) After 7 hours of seed liquid culture, turn on the primary fermentation tank raw material circulation pump, make the raw material enter the 2 / 3 volume of the fermentation tank, close the pump, and insert the seeds in the No. 1 seed tank into the pr...

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Abstract

The invention provides an acetone-butanol in-situ extraction continuous fermentation device and technology. The device comprises an No.1 seeding tank and an No.2 seeding tank which are alternately inoculated. Acetone-butanol fermentation is divided into two stages to continuously operate, wherein the first stage is conventional acid production period fermentation, and the second stage is in-situ extraction product synthesis period fermentation; the second period adopts a method of coupling extraction and fermentation and is matched with low-speed stirring to furthest lower product inhibiting effect and greatly improve butanol fermentation efficiency; and finally, static phase processing realizes the initial separation of mash and fermentation products. The invention has the advantages that the device and the technology have good extraction effect in production, can obviously improve butanol ratio in the fermentation product, can greatly lower production cost, and have obvious economic benefit. The technology is scientific and reasonable. The flow device is simple and easy to realize, can realize the new fermentation technology provided by the invention by slightly reforming the traditional butanol fermentation flow, and has wide application prospect.

Description

technical field [0001] The invention relates to acetone-butanol fermentation technology, in particular to a continuous fermentation device and process for in-situ extraction of acetone-butanol. Background technique [0002] Butanol is a comprehensive power fuel that can replace gasoline, and its physicochemical properties are closer to gasoline than ethanol. At present, the industrial production methods of butanol mainly include propylene oxo synthesis, aldol condensation and fermentation. The first two mainly use petroleum resources as raw materials, but with the increasing depletion of petroleum resources, this method is no longer suitable for large-scale industrial production. The production of butanol by fermentation method is mainly produced by anaerobic fermentation of Clostridium acetobutylicum. Grain (corn, corncob, rye, wheat) starch is used as raw material, water is added and mixed into mash, after cooking and sterilization, pure acetone butanol is added. Clostri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/02C12P7/16C12R1/145
Inventor 应明
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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