Method for producing reduced coenzyme Q10

A production method and reduction technology, applied in the field of biological production and coenzyme Q10 production, can solve the problems of molecular oxidation, environmental pollution of reduced coenzyme Q10, and harmful effects of coenzyme Q10

Inactive Publication Date: 2011-02-09
河南东都生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the reduced coenzyme Q10 produced by the above-mentioned process method will not only have environmental pollution problems in the production process of reduced coenzyme Q10 when it is applied to the fields of health food, beverage, cosmetics, and medicine, but also due to the presence of The problem of molecular oxidation, so when it is used as a functional additive of food, beverage, cosmetics, or as a component of pharmaceuticals to directly or indirectly affect the human body, its harm to the human body may be far greater than the positive effects of coenzyme Q10 on the human body. effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The human liver cell line Fa2N-4 (ATCC-5566) was selected as the parent material for the production of reduced coenzyme Q10.

[0027] From DMEM-1 ((high glucose) containing 4500mg / L D-glucose, L-glutamine and 25MmHEPES without sodium pyruvate and sodium bicarbonate) culture solution pH7.4, in 2.2L, DMEM-1 culture solution 3L cell generator, sterilized at 121°C for 10 minutes, add the mother material for the production of reduced coenzyme Q10-hepatic cell line Fa2N-4 (ATCC-5566) accounting for 10% of the volume of the culture medium, at 37°C, 5% Cultivate for 48 hours under the conditions of CO2 and 300 rpm stirring speed.

[0028] At the end of the cultivation, 30ml of 25% citric acid solution was added to the culture medium, and the 180g cell-containing wet body obtained by centrifuging the culture was collected and washed with water for 3 times to obtain a clean cell body.

[0029] Then, use a Mulitibeads shocker (multi-cell culture shaker), B300 to shake, stir for 1...

Embodiment 2

[0038] Human serum albumin was selected as the parent material for the production of reduced coenzyme Q1. From DMEM-1 ((high glucose) containing 4500mg / L D-glucose, L-glutamine and 2525Mm HEPES without sodium pyruvate and sodium bicarbonate) culture solution pH7.4, in 2.2L, DMEM-1 culture solution The 3L cell generator was sterilized at 121°C for 10 minutes, and 10% of the volume of the culture medium was added to the cell parent material for the production of reduced coenzyme Q10—human serum albumin. Cultivate for 48 hours at 37° C., 5% CO 2 , and a stirring rotation speed of 300 rpm.

[0039] At the end of the cultivation, 30ml of 25% citric acid solution was added to the culture medium, and the 180g cell-containing wet body obtained by centrifuging the culture was collected and washed with water for 3 times to obtain a clean cell body.

[0040]Then, use a Mulitibeads shocker (multi-cell culture shaker), B300 to shake, stir for 1 hour, break the cell body, and centrifuge to...

Embodiment 3

[0052] Chinese hamster ovary cells, CHO cells, were selected as the parent material for the production of reduced coenzyme Q10. From DMEM-1 ((high glucose) containing 4500mg / L D-glucose, L-glutamine and 25Mm HEPES without sodium pyruvate and sodium bicarbonate) culture solution pH7.4, in 2.2L, DMEM-1 culture solution The 3L cell generator was sterilized at 121°C for 10 minutes, and 10% of the volume of the culture medium was added to the parent material for the production of reduced coenzyme Q10—Chinese hamster ovary cell CHO cells. Cultivate for 48 hours at 37° C., 5% CO 2 , and a stirring rotation speed of 300 rpm.

[0053] At the end of the cultivation, 30ml of 25% citric acid solution was added to the culture medium, and the 180g cell-containing wet body obtained by centrifuging the culture was collected and washed with water for 3 times to obtain a clean cell body.

[0054] Then, use a Mulitibeads shocker (multi-cell culture shaker), B300 to shake, stir for 1 hour, break...

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Abstract

The invention relates to a method for producing a reduced coenzyme Q10. The method is characterized by comprising the following steps of: adopting cell lines or cell strains of all people and animals as female parents for producing the reduced coenzyme Q10; performing cell culture in biological culture agent DMEM-1 culture solution to obtain cell sap containing a great deal of the reduced coenzyme Q10; and obtaining a final product of the reduced coenzyme Q10 by a method of centrifugal separation and concentration or a method in which ethanol extraction and concentration is performed first and then cooling crystallization is performed. Compared with the conventional disclosed method for producing the reduced coenzyme Q10, the reduced coenzyme produced by the method is stable and is not oxidized by oxygen in the air, not only has no residue of a reducing agent but also has no chemical residues of any antioxidant, protective agent and the like, can be used for foods, medicaments, cosmetics or injections for infusion, and has no immunogenicity reaction to a human body.

Description

technical field [0001] The invention relates to a coenzyme Q10 production technology, in particular to a method for producing reduced coenzyme Q10 with animal cells, which belongs to the biological production technology category. Background technique [0002] Coenzyme Q10, also known as ubiquinone 10, is a fat-soluble quinone. It is an orange-yellow crystal at room temperature, with a melting point of 48°C. It is odorless and tasteless. Its structure is similar to vitamin K. The degree of polymerization of polyisoprenyl is 10 (named), which is a quinone compound. Another form of it is reduced coenzyme Q10. Its physiological functions are mainly derived from the redox properties of quinone groups and the physical properties of isoprenoid side chains. It has two main functions in human physiological skills: one is It has obvious anti-lipid peroxidation effect; second, it plays an important role in the process of converting nutrients into energy in mitochondria. Its quinone r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/91C12P7/66
Inventor 熊军宋敬民娄彩玲
Owner 河南东都生物科技有限公司
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