Preparation method of chrysoeriol

A technology of coirisin and medicinal materials, which is applied in the field of coirisin preparation, can solve the problems of low extraction rate and large loss of coirisin, and achieve the effects of short extraction time, easy control and prolonged service life

Inactive Publication Date: 2011-02-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the extraction and preparation process of coyilisin, which mainly involves the crude extraction of the phytochemical components in the research process, the organic solvent extraction, repeated silica gel column chromatography separation, and the determination of the separated compounds by mass spectrometry structure, the extraction rate of coyilisu separated by this method is low, and the loss is relatively large

Method used

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  • Preparation method of chrysoeriol

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Take 10 kg of Artemisia wilt leaf powder, add 80 L of pH10 sodium hydroxide aqueous solution, and perform ultrasonic extraction at 45°C for 30 minutes, filter and then use 40 L of pH10 sodium hydroxide aqueous solution to ultrasonically extract once, and combine the two filtrates. The pH of the filtrate was adjusted to neutrality with hydrochloric acid, centrifuged at 3000 r / min for 15 minutes, the precipitate was added with an appropriate amount of distilled water to make a 1L suspension, and 1L of chloroform was added to the suspension for continuous ultrasonic extraction, and the extraction was performed twice. The lower layer was removed, and the upper layer was continuously ultrasonically extracted twice with 1L of ethyl acetate. The four extractions were combined for a total of 4L, and were then subjected to microfiltration, ultrafiltration and nanofiltration in a multifunctional membrane separation device. The filter membrane material is a polyvinylidene fluoride ...

Embodiment 2

[0030] Take 10 kg of peony seed powder, add 100 L of sodium hydroxide aqueous solution with pH 9, ultrasonically extract at 50 ° C for 1 hour, filter and then use 50 L of sodium hydroxide aqueous solution with pH 9 to ultrasonically extract once, and combine the two filtrates. The filtrate was adjusted to neutral pH with hydrochloric acid, centrifuged at 3000r / min for 10 minutes, the precipitate was added with an appropriate amount of distilled water to make a 1L suspension, and 1L of chloroform was added to the suspension for continuous ultrasonic extraction, and the extraction was performed twice. The lower layer was removed, and the upper layer was continuously ultrasonically extracted twice with 1L of ethyl acetate. The four extractions were combined for a total of 4L, and microfiltration, ultrafiltration and nanofiltration were carried out in sequence in a multifunctional membrane separation device. The operating pressure was 1.5bar, wherein The microfiltration membrane is...

Embodiment 3

[0032] Take 50 kg of chrysanthemum brain whole herb powder, add 600 L of pH10 sodium hydroxide aqueous solution for 1.5 hours at 60°C for ultrasonic extraction, filter and then ultrasonically extract once with 300 L of pH10 sodium hydroxide aqueous solution, and combine the two filtrates. The filtrate was adjusted to neutral pH with hydrochloric acid, centrifuged at 3000r / min for 10 minutes, the precipitate was added with an appropriate amount of distilled water to make a 5L suspension, and 5L of chloroform was added to the suspension for continuous ultrasonic extraction, and the extraction was performed twice. The lower layer was removed, and the upper layer was continuously ultrasonically extracted twice with 5L of ethyl acetate. The four extractions were combined for a total of 20L, and microfiltration, ultrafiltration and nanofiltration were carried out successively in a multifunctional membrane separation device. The operating pressure was 1.5bar, wherein The microfiltrati...

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Abstract

The invention provides a preparation method of chrysoeriol. A high-purity chrysoeriol product is obtained by the steps of alkali extraction and water precipitation, organic solvent extraction, membrane concentration, macroporous resin adsorption, and the like. In a membrane separation process, not only the product purity is improved, but also the damage to resin is alleviated, and the service life of the resin is prolonged; and by adopting crystallization and recrystallization, the purification efficiency is high. The whole preparation process has the advantages that requirements on environment and equipment are low, extraction and separation time is short, purity is high and the preparation method is simple and easy to operate.

Description

Technical field: [0001] The invention belongs to the field of medicine and chemical industry, and relates to a preparation method of coelisol. Background technique: [0002] Coelisu [0003] English name: Chrysoeriol, molecular formula: C 16 H 12 O 6 ; Molecular weight: 300.26; [0004] Molecular structural formula: [0005] [0006] Coelitin is a pale yellow powder, mp. 320-322°C (mp. 336-337°C is also reported). Soluble in chloroform, ethyl acetate and other organic solvents. [0007] Medicinal effects: anti-tumor (inhibits the carcinogenic effect of 3,4-benzopyrene, inhibits the metabolism of benzopyrene); cytotoxicity (P388, ED 50 =1.9 μg / ml); cAMP phosphodiesterase inhibitor (in vitro, IC 50 = 269 μmol / L); anti-allergic and anti-inflammatory (inhibit basophils to release histamine, inhibit neutrophils to release β-glucuronidase); aldose reductase inhibitor (10 μmol / L inhibition rate 31.3%); xanthine Oxidase inhibitor (50μg / ml inhibition rate 61.5%, IC 50 = ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/40C07D311/30
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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