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Method for extracting corynoline

An extraction method and a technology for Corydalin, which are applied in the field of extraction of Corydalin, can solve problems such as few methods for extracting Corydalin, and achieve the effects of high product yield, reduced dosage, and improved processing capacity and effect.

Inactive Publication Date: 2011-02-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] At present, there are few public methods for extracting Corydalis

Method used

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  • Method for extracting corynoline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take kudiding medicinal material and grind it into 80 meshes, take 1kg and add 5 times the amount of 90% methanol for ultrasonic extraction for 1 hour, extract 3 times, filter the extract, recover methanol under reduced pressure with a rotary evaporator, add 100g of alumina to a small volume and mix well Dry (medium alumina is neutral alumina, particle size 120-200 mesh), crush and add 500ml acetone for ultrasonic extraction 3 times, extract acetone with rotary evaporator and dry. Take chloroform, methanol, and water to prepare a high-speed countercurrent chromatography solvent system in a ratio of 25:35:17. After shaking and shaking in a separatory funnel, the upper phase is used as the mobile phase to dissolve the dry matter, and the lower phase is used as the stationary phase. The flow rate was 2ml / min, the reverse rotation speed was 700r / min, the above crude extract was separated by high-speed countercurrent chromatography, the fraction of Corydalis was collected, th...

Embodiment 2

[0025] Take kudiding medicinal material and crush it into 40 meshes, take 1 kg and add 8 times the amount of 80% methanol for ultrasonic extraction for 1 hour, extract twice, filter the extract, recover methanol under reduced pressure with a rotary evaporator, add 150 g of alumina to a small volume and mix well Dry (medium alumina is neutral alumina, particle size 120-200 mesh), crush and add 800ml of 80% acetone for ultrasonic extraction twice, extract acetone with a rotary evaporator and dry. Take chloroform, methanol, and water to prepare a high-speed countercurrent chromatography solvent system in a ratio of 27:37:20. After shaking and layering in a separating funnel, take the upper phase as the mobile phase to dissolve the dry matter, and take the lower phase as the stationary phase. The flow rate was 3ml / min, the reverse rotation speed was 800r / min, the above crude extract was separated by high-speed countercurrent chromatography, the fraction of Corydalis was collected, ...

Embodiment 3

[0027] Take Kudiding medicinal material and crush it into 60 meshes, take 2kg and add 5 times the amount of 90% methanol for ultrasonic extraction for 1 hour, extract 3 times, filter the extract, recover methanol under reduced pressure with a rotary evaporator, add 200g of alumina and mix evenly to a small volume Dry (medium alumina is neutral alumina, particle size 120-200 mesh), pulverize, add 1000ml acetone and ultrasonically extract 3 times, use rotary evaporator to recover acetone from the extract until dry. Take chloroform, methanol, and water to prepare a high-speed countercurrent chromatography solvent system in a ratio of 25:30:17. After shaking and layering in a separating funnel, take the upper phase as the mobile phase to dissolve the dry matter, and take the lower phase as the stationary phase. The flow rate was 2ml / min, the reverse rotation speed was 700r / min, the above crude extract was separated by high-speed countercurrent chromatography, the fraction of Coryda...

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Abstract

The invention provides a method for extracting corynoline, which comprises the following steps of: crushing bunge corydalis herb, adding 80 to 90 percent methanol in an amount which is 5 to 10 times mass of the bunge corydalis herb for ultrasonic extraction for 2 to 3 times, filtering extracting solution, recovering the methanol under reduced pressure until a mall volume, adding alumina, mixing uniformly, drying, and adding acetone for ultrasonic extraction, recovering reagents from the extracting solution to obtain dried substances, selecting a chloroform-methanol-aqueous solvent system, and adopting a high-speed counter current chromatograph to separate the dried substances so as to obtain the high-content corynoline. The method for extracting the corynoline has low energy consumption and is environment-friendly.

Description

Technical field: [0001] The invention relates to a method for extracting Corydalis, in particular to a method for separating Corydalis by means of high-speed countercurrent chromatographic separation. Background technique: [0002] Corydalis, an isoquinoline alkaloid. [0003] Molecular formula: C21H21NO5 [0004] [0005] Physical and chemical properties: colorless cubic crystal, melting point 218°C-219°C, easily soluble in organic solvents such as chloroform and acetone. [0006] Kudiding, also known as Diding, Kuding, and Xiaojicai, is the rooted whole plant of the poppy family Viola corydalis. Traditional Chinese medicine is used to clear away heat and detoxify, promote blood circulation and reduce swelling, treat colds, coughs, hepatitis, edema, diarrhea and various inflammations, especially for rheumatism and myocarditis. Corydalis is the main index component of Kudiding medicinal material, "Chinese Pharmacopoeia" has clarified the content of Corydalis in Kudidin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/153
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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