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Method for preparing spherical bacterial cellulose

A bacterial cellulose and spherical technology, applied in the field of microbial fermentation, can solve the problems of harsh medium requirements, high culture costs, and long culture cycles, and achieve the effects of simple process, convenient operation, and reduced energy consumption

Active Publication Date: 2011-02-23
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cultivation of spherical bacterial cellulose has been reported for a long time, it has not been industrialized due to the problems of long cultivation period, high cost of continuous dynamic cultivation, and uneven particle size of spherical particles.
[0004] Chinese patent CN101586134A discloses a method for preparing spherical bacterial cellulose by using coconut water, vegetable juice, etc. shape) can be regulated according to fermentation conditions and time, but this method has strict requirements on the medium, and its main raw materials are easily affected by factors such as seasons, which is not conducive to industrial production
Chinese patent CN101705222A discloses a method for preparing bacterial cellulose microspheres for enzyme immobilization by continuous dynamic culture, but it is difficult to prepare spherical bacterial cellulose with larger particle size by using this method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Spherical bacterial cellulose was prepared by Acetobacter xylinum, and the activated strain was placed in a seed medium at 28° C., 80 rpm, and shaken on a shaker for 36 hours to obtain a seed solution. The seed medium is 100 g of glucose, 0.1 g of calcium carbonate and 0.1 g of yeast extract per liter of water.

[0024] (2) Adding the cultured seed solution into the fermentation medium, and adopting dynamic culture for 3 days to obtain spherical bacterial cellulose with uniform particles. The fermentation medium is 30 g of glucose, 1 g of disodium hydrogen phosphate, 1 g of potassium dihydrogen phosphate, 1 g of yeast extract, 1 g of peptone and 30 g of gelatin per liter of water. The fermentation medium accounts for 50% of the volume of the culture vessel, and the fermentation culture temperature is 28°C. The dynamic method adopted in the fermentation culture is continuous shaking on a shaking table, and the shaking frequency of the shaking table is 250 rpm.

[0...

Embodiment 2

[0027] (1) Spherical bacterial cellulose was prepared from Rhizobium, and the activated strain was placed in a seed medium at 30° C., 100 rpm, and shaken for 12 hours to obtain a seed solution. The seed medium is 30g of glucose, 1g of calcium carbonate and 1g of yeast extract per liter of water.

[0028] (2) Put the cultured seed solution into the fermentation medium, and adopt dynamic culture for 5 days to obtain spherical bacterial cellulose with uniform particles. The fermentation medium is 100 g of glucose, 4 g of disodium hydrogen phosphate, 4 g of potassium dihydrogen phosphate, 5 g of yeast extract, 5 g of peptone, and 20 g of gelatin per liter of water. The fermentation medium accounts for 70% of the volume of the culture vessel, and the fermentation culture temperature is 30°C. The dynamic method adopted in the fermentation culture is intermittent shaker shaking, and the shaking frequency of the shaker is 80rpm.

[0029] (3) The bacterial cellulose obtained by ferme...

Embodiment 3

[0031] (1) Spherical bacterial cellulose was prepared from Sarcinina sp., and the activated strain was placed in a seed medium at 35° C., 250 rpm, and shaken for 24 hours to obtain a seed solution. The seed medium is 50 g of glucose, 0.5 g of calcium carbonate and 0.2 g of yeast extract per liter of water.

[0032] (2) Put the cultured seed solution into the fermentation medium, and adopt dynamic culture for 7 days to obtain spherical bacterial cellulose with uniform particles. The fermentation medium is 50 g of glucose, 0.2 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, 0.2 g of yeast extract, 0.2 g of peptone and 1 g of gelatin per liter of water. The fermentation medium accounts for 60% of the volume of the culture vessel, and the fermentation culture temperature is 35°C. The dynamic method adopted in the fermentation culture is continuous mechanical stirring, and the frequency of mechanical stirring is 100rpm.

[0033] (3) The bacterial cellul...

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Abstract

The invention relates to the technical field of microorganism fermentation, in particular to a method for preparing spherical bacterial cellulose. The method for preparing the spherical bacterial cellulose comprises the following steps of: putting activated strains into a seed culture medium to carry out shaking culture by using a shaker so as to obtain seed solution; and filling the cultured seed solution into a fermentation medium and performing dynamic culture for 3 to 10 days to obtain the evengrained spherical bacterial cellulose. The method for preparing the spherical bacterial cellulose effectively reduces energy consumption in the culturing process by adopting the dynamic culture mode, can realize the regulation and control of the particle size of particles, can meet the requirements of the spherical bacterial cellulose applied in the fields of food processing, medicament sustained release, biomarker and the like, accords with the using requirement in the fields of food processing and biomedicine, has the characteristics of simple and easy process, convenience of operation, no pollution, low cost and the like and provides an effective approach for the industrial productionof the spherical bacterial cellulose.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing spherical bacterial cellulose, in particular to a method for obtaining spherical bacterial cellulose with uniform particle size distribution through dynamic culture. Background technique [0002] As an excellent biomaterial, bacterial cellulose (BC) has unique physical and chemical properties: BC has a natural three-dimensional nano-network structure; high tensile strength and elastic modulus; high hydrophilicity, good Excellent breathability, water absorption, water permeability, extraordinary water holding capacity and high wet strength. It tastes crisp and smooth, full of elasticity, good chewiness, and can still maintain the original quality after high temperature treatment. It has been widely used in food processing fields such as jelly, canned food, and beverages. Long-term consumption can promote gastrointestinal motility and prevent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/05C12R1/02C12R1/065C12R1/01C12R1/41C12P19/04C12R1/38
Inventor 钟春燕
Owner 钟春燕
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