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Method for extracting flavone from kudzuvine root fermentation butyl alcohol and residue thereof

A technology for fermenting butanol and residues, which is applied in the field of utilization of kudzu resources, can solve the problems of difficult industrialization of kudzu resources, lack of comprehensive utilization of raw materials, etc.

Inactive Publication Date: 2015-07-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the utilization of kudzu root resources, the existing utilization methods often only consider the utilization of a single component (starch or flavone) in the raw material, and the processing process is also a separate starch extraction process or flavonoid extraction and purification process, which lacks comprehensive utilization of raw materials. , leading to difficulties in the industrialization of kudzu root resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Get 50g of kudzu root (wherein the kudzu root starch content is 56%), add 200mL of distilled water, adjust to pH 6.5 with NaOH, sterilize at 121°C for 20min, then add 800mL of sterilized yeast powder containing 6g / L, 2.938 g / L K 2 HPO 4 and 1.768g / L KH 2 PO 4 Add 5% (v / v) seed solution to the culture solution, and anaerobically cultivate it at 37°C for 3 days; separate the culture medium from solid-liquid to obtain fermentation broth and fermentation residue, and use gas chromatography to measure the total solvent content in the fermentation broth. Butanol content is 4.47g / L; Acetone content is 2.16g / L; Weigh 2g of fermented residue (dry weight), extract and reflux 3 times at 80°C with 30ml of 80% ethanol, 2 hours each time; the extraction amount of flavonoids measured was 3.012g / 100g of kudzu root dry material, which was 12% higher than the extraction amount of flavonoids directly extracted from kudzu root.

Embodiment 2

[0024] Embodiment 2: Get Pueraria 100g (wherein Pueraria starch content is 56%), add 200mL distilled water, adjust to pH6.5 with NaOH, sterilize at 121 ℃ for 20min, then add 800mL sterilized containing 6g / L peptone, 2.938 g / LK 2 HPO 4 and 1.768g / LKH 2 PO 4 Add 5% (v / v) seed solution to the culture solution, and anaerobically cultivate it for 4 days at 37°C; separate the culture medium from solid-liquid to obtain fermentation broth and fermentation residue, and measure the total amount of solvent in the fermentation broth by gas chromatography It is 13.23g / L; Butanol content is 8.69g / L; Acetone content is 3.76g / L; Take 2g fermented residue (dry weight), use the ethanol of 30ml of 80% to extract and reflux 3 times at 80 ℃, every Second 2h; The extraction amount of flavonoids measured was 2.965g / 100g of kudzu root dry material, which was 11.8% higher than the extraction amount of flavonoids directly extracted from kudzu root.

Embodiment 3

[0025] Example 3: Get 140g of kudzu root (wherein the kudzu root starch content is 56%), add 200mL of distilled water, adjust to pH6.0 with NaOH, sterilize at 121°C for 20min, then add 800mL of sterilized solution containing 4.3g / L ammonium acetate , 2.938g / L K 2 HPO 4 and 1.768g / L KH 2 PO 4Add 10% (v / v) of the seed liquid to the culture solution, and anaerobically cultivate it for 4 days at 37°C; separate the culture medium from solid-liquid to obtain the fermentation liquid and fermentation residue, and measure the total amount of solvent in the fermentation liquid by gas chromatography It is 21.16g / L; Butanol content is 13.28g / L; Acetone content is 6.53g / L; Take by weighing 2g fermented residue (dry weight), use the ethanol of 30ml of 80% to extract and reflux 3 times at 80 ℃, every Second 2h; The extraction amount of flavonoids measured is 2.950g / 100g of kudzu root dry material, which is 11.7% higher than the extraction amount of flavonoids directly extracted from kudzu...

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Abstract

The invention relates to a method for extracting flavone from kudzuvine root fermentation butyl alcohol and residue thereof. Kudzuvine root is used as a raw material, digestion and saccharification processes are not needed, the butyl alcohol can be directly fermented, and the fermentation level of corn starch is reached. Compared with a method for directly extracting the flavone from the kudzuvine root, the method for extracting the flavone from the kudzuvine root fermentation residue improves the extraction quantity by 12 percent; and the kudzuvine root is pretreated by steam explosion technology and then fermented, so the flavone extraction quantity in the fermentation residue is improved by 15 percent. By the method, a new raw material is provided for production of biofuel butyl alcohol, the problem of high cost for fermenting the butyl alcohol by using the conventional grain starch is solved, and a new direction is opened for the comprehensive utilization and industrialized development of kudzuvine root resources.

Description

technical field [0001] The invention belongs to the utilization field of kudzu root resources, in particular to a method for extracting flavonoids from kudzu root by fermenting butanol and its residue. Background technique [0002] Butanol is an important solvent and a key ingredient in products such as paints, plastics and rubber. In recent years, butanol has also been considered as a new type of biofuel with great potential. Compared with methanol and ethanol, butanol has good water insolubility, low vapor pressure, high heat ratio, etc. It can achieve a higher mixing ratio with gasoline and is more suitable for use in existing fuel supply and distribution systems. Clostridium, represented by Clostridium.acetobutylicum, can produce a mixture of three main components, acetone, butanol, and ethanol, called a solvent. Traditional butanol fermentation substrates are corn and wheat, and the production cost is relatively high. As my country's demand for energy has intensified...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C07G99/00C12R1/145
Inventor 陈洪章王岚
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI